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Prussian blue producing strain and prussian blue producing process with the strain

A strain and common technology, applied in fungi, fermentation and other directions, can solve the problems of high price and low yield, and achieve the effects of low cost, high yield and good viscoelasticity

Inactive Publication Date: 2005-04-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, only the Japanese Hayashibara Company has a small amount of production, and the price is expensive
The primary reason restricting the mass production of pullulan is that during the fermentation process, Aureobasidium pullulans will secrete a black or dark green substance similar to melanin pigmentation, which firmly adheres to pullulan , it is difficult to remove by general method, it can be removed by Sevag fractionation method, but to completely remove melanin, it must be repeated many times, and each fractionation will lose about 15% of polysaccharides, and the final yield is very low, so low pigment strains must be screened , to overcome the influence of melanin on polysaccharide products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 10 liter fermenter culture

[0040] The strain is CGMCC No.1234;

[0041] Seed medium in g / L: sucrose 50, (NH 4 ) 2 SO 4 0.8,K 2 HPO 4 4. MgSO 4 0.2, NaCl0.2, yeast extract 1.5; pH6; sterilized at 121°C for 30 minutes;

[0042] The fermentation medium is calculated in g / L: sucrose 50, (NH 4 ) 2 SO 4 0.6,K 2 HPO 4 6. MgSO 4 0.4, NaCl 0.2, MnCl 20.005, yeast paste 0.4, pH 6.5; sterilized at 121°C for 30 minutes;

[0043] Seed culture conditions: Take a ring of slant strain cultured at 28°C for 4 days, inoculate it into the seed medium, fill the liquid volume with 100mL-120mL in a 500mL Erlenmeyer flask, and culture it at 28°C and 200r / min for 36 hours to obtain 120mL seed liquid;

[0044] 10-liter fermenter culture conditions: 10-liter fermenter, the liquid volume is 6 liters, the inoculation amount is calculated as 2%, which is exactly 120mL seed liquid; the temperature is 28°C, and the tank pressure is 0.5kg / cm 2 , the stirring speed is 400r / min, t...

Embodiment 2

[0046] 1.5m 3 fermenter culture

[0047] Seed medium, seed culture conditions, and fermentation medium are the same as in Example 1.

[0048] 1.5m 3 Fermentation tank culture conditions: 1.5m 3 Fermentation tank, the liquid volume is 900 liters, the inoculum volume is calculated as 2%, which is 18 liters of seed liquid, the temperature is 28 °C, and the tank pressure is 0.5 kg / cm 2 , the stirring speed is 400r / min, the ventilation rate is 3L / min, the fermentation time is 96 hours, the polysaccharide yield reaches 25.6g / L, and the sugar conversion rate reaches 51.2%.

Embodiment 3

[0050] Carry out post-treatment with the fermented liquid obtained in Example 1 or Example 2, post-treatment conditions are: fermented liquid is filtered I, centrifugal I, heat treatment, centrifugal II, decolorization, centrifugal III, industrial alcohol precipitation, centrifugal IV, desalination, filter II Obtain a liquid pullulan product; or continue drying to obtain a solid pullulan product.

[0051] The specific operation is:

[0052] Filtration I: 8 layers of gauze suction filtration,

[0053] Centrifugal I: 3000r / min, 30min,

[0054] Heat treatment: The supernatant after centrifugation I is used for heat treatment, with NaHCO 3 Adjust the pH value with HCl, 70°C, 10min, pH7.0, before heat treatment, the absorbance is 0.295, after heat treatment, the absorbance is 0.300; before heat treatment, the polysaccharide content is 3 1.5g / L, after heat treatment, the polysaccharide content is 29.8g / L L, the polysaccharide yield was 94.5%.

[0055] Centrifugal II: 4000r / min, ...

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PUM

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Abstract

The present invention relates to utilizing saccharide metabolism to produce extracellular water soluble polysaccharide and belongs to the field of bioengineering technology. The present invention provides strain, named Aureobasidium pullulans G-58 and with the preservation number of CGMCC No. 1234. The pullulan producing process with the strain includes fermentation culture, fermented liquid post-treatment, heat treatment, decolorizing, precipitation, desalting, drying and other steps. The pullulan product has light color, high polysaccharide content, excellent viscoelasticity, excellent emulsifying performance and plasticity, and no toxicity and harm, and may be used widely in food, medicine, cosmetics, etc.

Description

technical field [0001] The invention discloses a strain for producing pullulan and a method for producing pullulan by using the strain, involving the production of extracellular water-soluble polysaccharide by utilizing sugar metabolism, and belongs to the technical field of bioengineering. Background technique [0002] Pullulan is officially named pullulan, also known as pullulan or pullulan, which is an extracellular water-soluble polysaccharide produced by Aureobasidium pullulans during the cultivation process using sugar metabolism. Its molecule is a linear polysaccharide formed by connecting maltotriose with α-1,6-glycosidic bonds. The ratio of 1,4 bonds to 1,6 bonds in the molecule is 2:1, the degree of polymerization is 100-5000, and the molecular weight is (4~200)×104. Due to this unique connection method, pullulan has excellent viscoelasticity, emulsification, plasticity, non-toxic and harmless, and is widely used in food, medicine, cosmetics and other industries. ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/10
Inventor 童群义付湘晋于航
Owner JIANGNAN UNIV
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