6 results about "Microbial toxins" patented technology

The invention discloses a small-sized mixed water-washing wheat washing machine, and relates to the technical field of wheat washing. The device comprises a bottom pool, the bottom pool is composed of a hydrogen peroxide pool, a first collecting pool and a second collecting pool which are distributed from right to left, two outer baffles are fixedly installed at the top of the bottom pool, an inner conveying line body is arranged between the two outer baffles, a feeding hopper is erected at the tops of the two outer baffles, and high-pressure flushing assemblies are arranged on the inner walls of the two outer baffles; the high-pressure washing assembly is located over the second collecting pool, a centrifugal dewatering assembly is arranged on the left side of the bottom pool, a negative-pressure conveying assembly is arranged at the top of the centrifugal dewatering assembly, and the negative-pressure conveying assembly can convey the wheat located on the second sinking part into the centrifugal dewatering assembly and can suck out the dewatered wheat in the centrifugal dewatering assembly. By innovatively designing the multi-stage cooperative processing module, efficient degradation and removal of various microbial toxins in wheat are achieved, and meanwhile the wheat processing quality is guaranteed.

The present application relates to a kind of antigen binding protein pair specifically binding enterotoxin SEC and its application.The antigen binding protein pair of the present application includes the first antigen binding protein 16E12 combined with enterotoxin SEC and the second antigen binding protein 9B7 combined with enterotoxin SEC combined with the first antigen binding protein 16E12.The antigen binding protein of the present application has unique variable region sequence, the minimum detection limit for enterotoxin SEC is 0.1ng / mL, and there is no cross reaction for enterotoxin SEA, SEB, SED, SEE and common microbial toxin;The antigen binding protein sequence of the present application is clear, can effectively avoid gene mutation, the batch difference of antigen binding protein is small, quality is stable, structure is complete, it is helpful to the development of stable immune analysis detection method.

The invention belongs to the technical field of microbiological detection, and relates to a handheld detector based on green fluorescent probe detection and a detection method and application thereof. The detector comprises a sample fusion assembly and a fluorescence collection assembly, the sample fusion assembly comprises a reagent tube, a swab and a handheld cap, and the top end of the swab is fixed in the handheld cap to form a sampling assembly; a squeezing-breaking assembly is mounted at the top of the sample tube, the test tube penetrates through the squeezing-breaking assembly, the squeezing-breaking assembly squeezes the test tube to squeeze and break the swab, and the swab falls into the test tube and is fused with the detection solution; a fluorescence collection assembly is arranged on the side of each sample fusion assembly, and after a sampled swab falls into a reagent tube, the swab can be subjected to fusion reaction with a detection solution, fluorescence excitation is carried out, a fluorescence sensor acquires change of a fluorescence signal, and the concentration of a pollutant to be detected is directly output after the change of the fluorescence signal is processed by a main control board. The concentration of the microbial toxins in the sample to be detected can be rapidly detected, the sample does not need to be sent to a professional institution for detection, and public users can perform detection by themselves.

The invention relates to an antigen binding protein pair specifically binding to enterotoxin SEC and application of the antigen binding protein pair. The antigen-binding protein pair of the present invention comprises a first antigen-binding protein 16E12 that binds to enterotoxin SEC and a second antigen-binding protein 9B7 that binds to enterotoxin SEC to which the first antigen-binding protein 16E12 is bound. The antigen binding protein disclosed by the invention has a unique variable region sequence, the lowest detection limit of the antigen binding protein to enterotoxin SEC is 0.1 ng / mL, and the antigen binding protein has no cross reaction to enterotoxins SEA, SEB, SED and SEE and common microbial toxins; the antigen binding protein disclosed by the invention is clear in sequence, capable of effectively avoiding gene mutation, small in batch difference, stable in quality and complete in structure, and contributes to the development of stable immunoassay detection methods.

The invention relates to the technical field of environmental microbial toxin detection, in particular to a qPCR technology-based algal toxin production gene detection analysis method and system, and the method comprises the following steps: generating an original amplification curve; outputting an effective amplification curve after the PCR inhibition effect is eliminated; obtaining an algae toxin-producing gene database, performing dynamic baseline correction on the effective amplification curve, and outputting a baseline correction curve; calculating a second derivative of the baseline correction curve and positioning a global maximum point; based on the global maximum value point, determining a self-adaptive threshold value, and calculating a Ct value representing the expression level of the target gene; obtaining a toxin expression profile database, and constructing a gene interaction network based on the Ct value; the gene interaction network is analyzed, and the toxin production potential state is judged; and integrating the effective amplification curve, the Ct value, the gene interaction network and the toxin production potential state to generate a detection report. According to the method, the accuracy and the reliability of detecting the toxin-producing genes of the algae are remarkably improved.

The application relates to a pair of antigen binding proteins specifically combined with Bacillus cereus NheA, and relates to the field of biological immunization technology. The application obtains a pair of antigen binding proteins specifically combined with Bacillus cereus NheA through screening, and the pair of antigen binding proteins are named as 5C1 and 8G7 and have unique variable region sequences. The non-hemolytic enterotoxin Nhe A of Bacillus cereus is detected by using a double-antibody sandwich detection method with 5C1 as a capture antibody and 8G7 as a detection antibody, and the minimum detection limit is 0.8 ng / mL, which indicates that the recombinant antibody has good sensitivity to Nhe A. In addition, the recombinant antibody has no cross reaction to other microbial toxins and has good specificity.