Method for producing novel natural melanin
A technology of natural melanin and a production method, which is applied in the field of microbial liquid fermentation to produce melanin, can solve problems such as toxicity and many melanin reaction processes, and achieve the effects of improving utilization rate and reducing production cost.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0014] (1) Activation of strains: culture the strains preserved on the slant of the PDA medium at a constant temperature of 25° C. for 4 hours to obtain activated strains;
[0015] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous solution composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 1, distilled water, pH 4, sterilized at 121°C for 20 minutes; the shaker flask was cultivated on a shaker at 25°C with a rotation speed of 200r / min 24h, as seed solution;
[0016] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O 0.1, ZnSO 4 .7H 2 O 5×10 -5 , corn extract liquid 0.5, pH 4.5~5.0. 121 ℃ high-pressure steam sterilization 20min; FeSO 4 0.02, distilled water, pH 4, sterilized at 121°C for 20min.
[0017] (4) Seed...
Embodiment 2
[0020] (1) Activation of strains: the strains preserved on the slant of the PDA medium were incubated at 25°C for 6 hours at a constant temperature to obtain activated strains;
[0021] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous solution composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 1, distilled water, pH 4, sterilized at 121°C for 20 minutes; the shaker flask was cultivated on a shaker at 25°C with a rotation speed of 200r / min 24h, as seed solution;
[0022] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O0.1, ZnSO 4 .7H 2 O5×10 -5 , corn extract 0.5, pH 4.5~5.0. 121 ℃ autoclave steam sterilization 20min; 4 0.05, distilled water, pH 7, sterilized at 121°C for 20min.
[0023] (4) Seed liquid is inocu...
Embodiment 3
[0026] (1) Activation of strains: the strains preserved on the slant of the PDA medium were incubated at 25°C for 6 hours at a constant temperature to obtain activated strains;
[0027] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 3, distilled water, pH 4.5, sterilized at 121°C for 20 minutes; shake the flask at 25°C, and cultivate it on a shaker with a rotation speed of 200r / min 24h, as seed solution;
[0028] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O0.1, ZnSO 4 .7H 2 O5×10 -5 , corn extract 0.5, pH 4.5~5.0. 121 DEG C high-pressure steam sterilization 20min; FeSO 4 0.02, distilled water, pH 4.5, sterilized at 121°C for 20min. (4) Seed liquid is inocu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com