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Method for producing novel natural melanin

A technology of natural melanin and a production method, which is applied in the field of microbial liquid fermentation to produce melanin, can solve problems such as toxicity and many melanin reaction processes, and achieve the effects of improving utilization rate and reducing production cost.

Active Publication Date: 2012-06-27
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis of melanin has many reaction processes and is toxic to a certain extent; the extraction of melanin from animals and plants has the problem of sample source

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Activation of strains: culture the strains preserved on the slant of the PDA medium at a constant temperature of 25° C. for 4 hours to obtain activated strains;

[0015] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous solution composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 1, distilled water, pH 4, sterilized at 121°C for 20 minutes; the shaker flask was cultivated on a shaker at 25°C with a rotation speed of 200r / min 24h, as seed solution;

[0016] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O 0.1, ZnSO 4 .7H 2 O 5×10 -5 , corn extract liquid 0.5, pH 4.5~5.0. 121 ℃ high-pressure steam sterilization 20min; FeSO 4 0.02, distilled water, pH 4, sterilized at 121°C for 20min.

[0017] (4) Seed...

Embodiment 2

[0020] (1) Activation of strains: the strains preserved on the slant of the PDA medium were incubated at 25°C for 6 hours at a constant temperature to obtain activated strains;

[0021] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous solution composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 1, distilled water, pH 4, sterilized at 121°C for 20 minutes; the shaker flask was cultivated on a shaker at 25°C with a rotation speed of 200r / min 24h, as seed solution;

[0022] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O0.1, ZnSO 4 .7H 2 O5×10 -5 , corn extract 0.5, pH 4.5~5.0. 121 ℃ autoclave steam sterilization 20min; 4 0.05, distilled water, pH 7, sterilized at 121°C for 20min.

[0023] (4) Seed liquid is inocu...

Embodiment 3

[0026] (1) Activation of strains: the strains preserved on the slant of the PDA medium were incubated at 25°C for 6 hours at a constant temperature to obtain activated strains;

[0027] (2) Take a ring of Aureobasidium pullulans and insert it into a 500 ml baffle bottle with 50 ml of culture medium. The aqueous composition of the seed medium is (g / L): glucose 20, potato juice 100, beef extract 3, distilled water, pH 4.5, sterilized at 121°C for 20 minutes; shake the flask at 25°C, and cultivate it on a shaker with a rotation speed of 200r / min 24h, as seed solution;

[0028] (3) Fermentation medium: the existing fermentation medium, its aqueous solution is composed of (g / L): glucose 100, ammonium succinate 3, succinic acid 2, K 2 CO 3 0.3, KH 2 PO 4 0.1, MgSO 4 .7H 2 O0.1, ZnSO 4 .7H 2 O5×10 -5 , corn extract 0.5, pH 4.5~5.0. 121 DEG C high-pressure steam sterilization 20min; FeSO 4 0.02, distilled water, pH 4.5, sterilized at 121°C for 20min. (4) Seed liquid is inocu...

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Abstract

The invention discloses a method for producing novel natural melanin. The method is characterized by comprising the following steps of: activating aureobasidium pullulans for 4 to 8 hours, performing shaking culture for 12 to 36 hours to obtain seed liquid, inoculating the seed liquid in a volume of which is 1 to 10 percent of that of a fermentation medium to the fermentation medium, and performing liquid state fermentation; centrifuging the fermentation solution obtained by fermentation for 5 minutes under the condition of 5,000r / min, performing acidolysis on the supernate by using hydrochloric acid, and then performing precipitation by using methanol to obtain a crude melanin product; dissolving the crude product to obtain a black solution by using an alkali solution; and performing precipitation on the obtained black solution by using hydrochloric acid, and thus obtaining the melanin. Compared with other fermentation medium, the method using the fermentation medium has the advantages that: the yield of the melanin is improved from 2.47g / L to 10.74g / L, the fermentation period is shortened from 7 days to 3 days, the culture substrate is fully utilized, the utilization rate of raw materials is improved, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of producing melanin by liquid fermentation of microorganisms, and in particular relates to a strain of Aureobasidium pullulans and a method for improving the production of melanin by liquid fermentation by changing a culture medium. Background technique [0002] Melanin is a kind of natural pigment widely present in living organisms, and it forms irregular heterogeneous polyphenol-like polymers through the oxidation of polyhydric phenols (very easily bound to proteins). In industry and agriculture, melanin can be used as a cosmetic ingredient that absorbs ultraviolet rays, a component of natural hair dyes, a cation chelating agent, as an amorphous semiconductor and as a photoprotectant for biopesticides; in addition, melanin also has a wide range of medical applications. The scope of application, has the functions of scavenging free radicals, preventing the oxidation of cardiolipin liposomes, anti-radiation...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/645
Inventor 乔长晟李利宁敖爱华汪建明
Owner 天津北洋百川生物技术有限公司
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