Aureobasidium pullulans and applications thereof

A technology of Aureobasidium pullulans and chloramphenicol, applied in the directions of application, biochemical equipment and methods, organic fertilizers, etc., can solve problems such as the decline of agricultural product quality, soil and water source crop pollution, etc., to prevent the occurrence of diseases and increase soil fertility. , inhibition and disease occurrence effect

Active Publication Date: 2017-08-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of various diseases of crops is mainly based on pesticides and chemical preparations. The use of a large number of chemical fertilizers and pesticides will pollute the soil, water sources and crops, resulting in a decline in the quality of agricultural products.

Method used

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  • Aureobasidium pullulans and applications thereof
  • Aureobasidium pullulans and applications thereof
  • Aureobasidium pullulans and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Aureobasidium pullulans HJM-1 strain separation

[0025] The isolation method of Aureobasidium pullulans HJM-1 bacterial classification is specifically: the seawater, sea mud, seaweed and marine animal sample 2.0g that will be taken from Qingdao offshore are inoculated in 20.0ml chloramphenicol YPD liquid culture medium [containing 2.0% (w / v) peptone, 2.0% (w / v) glucose, 1.0% (w / v) yeast powder, 0.05% (w / v) chloramphenicol, pH 5.5, prepared in distilled water, sterilized at 115°C for 30min], in Shake culture at 25-28°C for 2-3 days. The culture solution was serially diluted 10 times with sterile 0.85% NaCl solution to 10 -1 to 10 -6 , take 1-3 suitable dilutions and coat 3 chloramphenicol YPD plate medium [containing 2.0% (w / v) peptone, 2.0% (w / v) glucose, 1.0% (w / v) for each dilution / v) Yeast powder, 0.05% (w / v) chloramphenicol, 2.0% (w / v) agar powder, pH 5.5, prepared with distilled water, sterilized at 115°C for 30min], each plate was coated with 1...

Embodiment 2

[0031] Example 2: Preparation of marine microbial fertilizer

[0032] The method for preparing microbial fertilizer by Aureobasidium pullulans HJM-1 fermentation comprises the following steps:

[0033] (1) Slant culture: inoculate the strain with the preservation number CGMCC No.12249 in the YPD slant medium, and cultivate it in an incubator at 25-28°C for 2-3 days;

[0034] (2) Erlenmeyer flask shake flask culture: inoculate the cultured slant strains into the YPD liquid medium of the Erlenmeyer flask according to the amount of 6%-10%, and cultivate them in a shaking incubator at 25-28°C for 2-3d;

[0035] (3) Amplified liquid culture in fermenter: inoculate the liquid bacteria into the fermenter according to the amount of 6%-10%, keep the temperature at 25-28°C during the fermentation process, the stirring speed at 200-240r / min, and the fermentation time for 2-3d, After the fermentation is finished, the liquid microbial fertilizer is obtained.

[0036] (4) Add the liquid m...

Embodiment 3

[0037] Embodiment 3: the application of the marine microbial bacterial fertilizer prepared in embodiment 2 on cucumber planting

[0038] Select cucumber seedlings with consistent growth and plant them in plastic flowerpots with a diameter of 15 cm, one plant per pot, apply microbial fertilizers with different treatment methods with the above-mentioned solid microbial fertilizer and the above-mentioned liquid microbial fertilizer ≥ 100 million / ml, and take the solid 40g of bacterial fertilizer is the base fertilizer treatment, liquid bacterial fertilizer of different dilutions is applied once every 5 days, 20ml each time, and 20ml of sterilized liquid YPD medium diluted 5 times is added as the control, and the plant height and diameter of cucumbers are measured 30 days after planting rough and compared with the control. The test results showed that the plant height and plant diameter of each experimental group treated with microbial fertilizer were significantly higher than tho...

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Abstract

The invention provides aureobasidium pullulans and applications thereof. The collection number of the strain is CGMCC No.12249, and marine yeast aureobasidium pullulans HJM01 is applied to agricultural production as a microbial fertilizer. The screened marine yeast aureobasidium pullulans and active substances in fermented liquor of the aureobasidium pullulans serve as the microbial fertilizer, the fertilizer can effectively inhibit and prevent the occurrence of diseases of botrytis cinerea on vegetables of tomatoes and the like after being applied to soil, the strain can decompose insoluble phosphate to produce soluble phosphate after producing acid in the soil, and phytase produced from the strain can decompose phytic acid in the soil to produce soluble phosphate, so that the soil fertility can be increased and the growth of plants can be promoted.

Description

technical field [0001] The invention relates to the technical field of microorganisms and microbial fertilizers, in particular to Aureobasidium pullulans and applications thereof. Background technique [0002] In my country's agricultural production, due to the long-term use of chemical fertilizers for many years, the proportion of various nutrients is out of balance, the ecological environment and microbial flora of the farmland are destroyed, and the soil is compacted, which affects the safety of agricultural products to a certain extent. At present, the prevention and control of various diseases of crops is mainly based on pesticides and chemical preparations. The use of a large amount of chemical fertilizers and pesticides will pollute the soil, water sources and crops, resulting in a decline in the quality of agricultural products. [0003] Compared with land, the marine environment is characterized by high salinity, high pressure, low temperature, and dilute nutrients....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C05F11/08C05G3/04C12R1/645C05G3/80
CPCC05F11/02C05F11/08C12N1/02C05G3/80C12N1/145C12R2001/645
Inventor 王祥红
Owner OCEAN UNIV OF CHINA
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