Method for co-production of oligose and dietary fiber through wheat bran fermentation

A technology of dietary fiber and oligosaccharides, which is applied in the field of co-production of oligosaccharides and dietary fibers from fermented wheat bran, can solve problems such as environmental pollution and waste of resources, and achieve the effects of no environmental pollution, low production costs, and reduced production costs

Inactive Publication Date: 2012-07-25
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has mild reaction and simple operation, but the removal of hydrolyzate causes waste of resources and forms a large amount of waste water
Therefore, there are problems such as waste of resources and environmental pollution in the previous preparation methods.

Method used

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  • Method for co-production of oligose and dietary fiber through wheat bran fermentation
  • Method for co-production of oligose and dietary fiber through wheat bran fermentation
  • Method for co-production of oligose and dietary fiber through wheat bran fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 Aureobasidium pullulans produces feruloyl oligosaccharides fermentation process optimization

[0014] 1. Preparation of culture medium

[0015] Strain activation medium (g / L): potato 200 sucrose 20 peptone 5 agar powder 20pH natural distilled water 1000mL 0.103MPa sterilized for 20min;

[0016] Seed medium (g / L): sucrose 50Na 2 HPO 4 5.0 yeast extract 3.0 MgS04 7H 2 O 0.2 (NH 4 ) 2 SO 4 0.6NaCl0.1 pH6.0 distilled water 1000mL 0.103MPa sterilization for 20min;

[0017] Fermentation medium (g / L): wheat bran liquid 20KH 2 PO 4 1 MgSO 4 ·7H 2 O 1 V B1 0.1 0.103MPa sterilization for 20min.

[0018] 2. Strain culture

[0019] Cultivation of mother species: at a temperature of 28°C, a shaker speed of 180r / min, and a liquid volume of 50mL / 250mL in a conical flask, inoculate a block of mother species with a diameter of about 4mm, and cultivate for 3 days;

[0020] Fermentation conditions for determining culture conditions: Based on the previous sin...

Embodiment 2

[0045] The orthogonal test of embodiment 2 mixed bacteria fermentation production xylanase

[0046] Select the four factors of wheat bran particle size, wheat bran treatment method, wheat bran water content, and bacterial strain combination method, and use the orthogonal test table to carry out four-factor three-level orthogonal experiments (Table 5), and optimize the production of xylanase. The best mixed culture combination.

[0047] Table 5 Orthogonal experiment factors and levels

[0048] Table 5 Factors and levels in orthogonal experiment

[0049]

[0050] Table 6 Orthogonal experimental design

[0051] Table 6 Orthogonal experimental design

[0052]

[0053] It can be seen from Table 6 that the effect of xylanase produced by solid-state fermentation of mixed bacteria on wheat bran is as follows: In the single factor test of medium, the degree of influence is in the order of wheat bran treatment method>bacteria combination method>wheat bran particle size>wheat br...

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Abstract

The invention relates to application of a microbial fermentation method in co-production of wheat bran oligose and dietary fiber. The invention aims to developed and utilize the wheat bran comprehensively; an efficient biological process route is adopted to deeply processing wheat bran, and the microbial fermentation method is used to prepare wheat bran dietary fiber and functional oligose simultaneously. The microbe used in the method refers to a single strain, pullularia pullulans or mixed strains of aureobasidium pulluans and agrocybe aegerita; in fermentation of wheat bran, substances in the wheat bran, such as starch and protein, can be taken as nutritional ingredients for the growth of microbes, when digesting the substances, the microbes can generate xylanase so as to hydrolyze wheat bran hemicellulose to generate oligose and prepare dietary fiber simultaneously. According to the method, the research and development idea of developing a single product is broken through, the rate of multipurpose utilization of the resource is high, the production costs is low, chemical substance residual does not exist, the environment is not polluted, a new processing technology model of 'ecological, low-carbon and efficient circulating' is formed, and the additional value of the wheat bran product is increased.

Description

technical field [0001] The invention relates to the application of biological method in preparing wheat bran oligosaccharide and dietary fiber. Background technique [0002] Cereal bran has attracted increasing research interest due to its important nutritional properties, and one of the main research objects is wheat bran. Bran is rich in dietary fiber (35-50%), protein (12-18%), fat (3-5%), total carbohydrate (45-65%), ash (4-6%), vitamin ( 0.265%) and natural antioxidant ferulic acid (0.4-0.7%) and other components, the development and utilization potential is huge. [0003] At present, the main research is to use wheat bran to process single products such as wheat bran dietary fiber, wheat bran protein and pentosan, such as removing starch by enzymatic hydrolysis, removing protein by acid hydrolysis to produce insoluble dietary fiber products, or by enzymatic hydrolysis, Alkali dissolution, alcohol precipitation and other processes produce pentosan, and some products s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P39/00A23L1/308C12R1/645
CPCY02A40/90
Inventor 余晓红顾振新邵荣刘珊珊申玉香
Owner YANCHENG INST OF TECH
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