Process for producing pullulan
A technology of seeds and strains, applied in the field of pullulan production, can solve the problems of pullulan being black or the deep pigmentation rate and low rate of pigmentation.
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Embodiment 1
[0019] The method for producing pullulan comprises the following steps:
[0020] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);
[0021] (2) Preparation of medium: add water and amylase to corn flour to liquefy, dissolve the carbohydrates in the liquefaction solution, separate the solid components in the liquefaction solution, and use the corn flour liquefaction solution to prepare seeds culture medium and fermentation medium;
[0022] Contains 0.21 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 4.0 g, MgSO 4 0.19 grams, 1.05 grams of NaCl, 1.49 grams of yeast extract, and the rest is corn flour liquefaction liquid with DE value of 50, pH value of 6.0, using H 2 SO ...
Embodiment 2
[0028] The method for producing pullulan comprises the following steps:
[0029] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);
[0030] (2) Preparation of culture medium: glucose is made into a solution for preparing seed culture medium and fermentation medium;
[0031] Containing 0.20 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 4.1 g, MgSO 4 0.21 grams, 1.01 grams of NaCl, 1.49 grams of yeast extract, the rest is a glucose solution with a DE value of 52, a pH value of 6.0, and H 2 SO 4 , Ca(OH) 2 as an acidity regulator;
[0032] Each kilogram of fermentation medium contains 0.41 grams of ammonia water, K 2 HPO 4 4.05 g, MgSO 4 0.41 grams, 1 gram of NaCl, ...
Embodiment 3
[0037] The method for producing pullulan comprises the following steps:
[0038] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);
[0039] (2) Preparation of medium: add water and amylase to corn flour to liquefy, dissolve the carbohydrates in the liquefaction solution, separate the solid components in the liquefaction solution, and use the corn flour liquefaction solution to prepare seeds culture medium and fermentation medium;
[0040] Contains 0.19 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 2.5 g, MgSO 4 0.16 grams, 0.95 grams of NaCl, 1.36 grams of yeast extract, and the rest is corn flour liquefaction liquid with DE value 49, pH value is 6.3, using H 2 SO 4 ,...
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