Process for producing pullulan

A technology of seeds and strains, applied in the field of pullulan production, can solve the problems of pullulan being black or the deep pigmentation rate and low rate of pigmentation.

Active Publication Date: 2005-08-17
广东富味健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem that the pullulan produced by the existing method for producing pullulan is black or very dark and the yield is generally not high, and proposes a method for producing pullulan, by cultivating a production strain with high yield and low pigment , Preparation of suitable culture medium and selection of suitable production process to realize the industrial production of light-colored pullulan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for producing pullulan comprises the following steps:

[0020] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);

[0021] (2) Preparation of medium: add water and amylase to corn flour to liquefy, dissolve the carbohydrates in the liquefaction solution, separate the solid components in the liquefaction solution, and use the corn flour liquefaction solution to prepare seeds culture medium and fermentation medium;

[0022] Contains 0.21 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 4.0 g, MgSO 4 0.19 grams, 1.05 grams of NaCl, 1.49 grams of yeast extract, and the rest is corn flour liquefaction liquid with DE value of 50, pH value of 6.0, using H 2 SO ...

Embodiment 2

[0028] The method for producing pullulan comprises the following steps:

[0029] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);

[0030] (2) Preparation of culture medium: glucose is made into a solution for preparing seed culture medium and fermentation medium;

[0031] Containing 0.20 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 4.1 g, MgSO 4 0.21 grams, 1.01 grams of NaCl, 1.49 grams of yeast extract, the rest is a glucose solution with a DE value of 52, a pH value of 6.0, and H 2 SO 4 , Ca(OH) 2 as an acidity regulator;

[0032] Each kilogram of fermentation medium contains 0.41 grams of ammonia water, K 2 HPO 4 4.05 g, MgSO 4 0.41 grams, 1 gram of NaCl, ...

Embodiment 3

[0037] The method for producing pullulan comprises the following steps:

[0038] (1) Breeding of strains: Use Aureobasidium pullulans as the starting strain for ultraviolet and nitrosoguanidine (NTG) mutagenesis, and screen for mutagenesis that secretes less melanin and has a higher transformation rate from a large number of mutagenized strains Bacterial strain and preservation (concrete steps are as described above);

[0039] (2) Preparation of medium: add water and amylase to corn flour to liquefy, dissolve the carbohydrates in the liquefaction solution, separate the solid components in the liquefaction solution, and use the corn flour liquefaction solution to prepare seeds culture medium and fermentation medium;

[0040] Contains 0.19 grams of ammonia water per kilogram of seed medium, K 2 HPO 4 2.5 g, MgSO 4 0.16 grams, 0.95 grams of NaCl, 1.36 grams of yeast extract, and the rest is corn flour liquefaction liquid with DE value 49, pH value is 6.3, using H 2 SO 4 ,...

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PUM

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Abstract

The pullulan producing process includes the following steps: 1) selectively breeding strain through ultraviolet and NTG inducing ureobasidium pullulans and screening out induced strain with less melanin secretion and high conversion rate; 2) compounding seed culture medium and fermentation culture medium; 3) culturing the obtained strain at 30-33 deg.c and inoculating in sterilized and cooled seed culture medium for culture to obtain seed liquid; 4) fermentation through setting sterilized and cooled fermentation culture medium and the obtained seed liquid in the inoculated amount of 1.5-5 % into fermentation tank to obtain fermented liquid; and 5) extracting pullulan product from the fermented liquid. The present invention has polysaccharide converting rate up to 60-70 g / L, pullulan polysaccharide yield up to 60-70 % and white or yellowish pullulan obtained.

Description

technical field [0001] The invention relates to a method for producing pullulan. Background technique [0002] In the chemical industry, food industry and pharmaceutical industry, it has long been desired to obtain an edible film that can be decomposed by soil microorganisms, does not produce high temperature or toxic gases after burning, has almost no change in physical properties to temperature differences, and is not easy to pass through oxygen. , acid and alkali-resistant high-strength adhesives and natural raw materials that are beneficial to the environment and can be used to produce valuable industrial products. The polysaccharide pullulan is suitable for these requirements. Pullulan is a new type of polymer biomaterial, which has excellent film-forming, fiber-forming, gas-barrier, adhesive, easy-processing, non-toxic properties, and has been widely used in food, medicine, light industry, chemical industry and oil and other fields. [0003] At present, most pullulan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04
Inventor 余少华喻敏林玉惠杨汉彬刘谋泉郑华丰黄鹤杨志军周晓娜陈远志李慧
Owner 广东富味健康科技有限公司
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