33results about How to "Improve substrate utilization" patented technology

The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.

The invention discloses a method for generating energy from hydrogen production bacteria and oily microalgae in a stair-coupling mode, and relates to the field of biological energy, and mainly aims at solving the problems that the conventional fermentation products in dark fermentation hydrogen production are hard to utilize, environment pollution is easy to cause, the substrate utilization rate is low and the energy production efficiency is low. The method comprises the following steps: firstly, taking the hydrogen production bacteria to culture, secondly, centrifuging an organic acid fermentation liquid, thirdly, inoculating the oily microalgae, and fourthly, extracting grease. According to the method, a dark fermentation hydrogen production technique is combined with a microalgae oil production technique, the problems that the fermentation products in the dark fermentation hydrogen production are hard to utilize and environment pollution is likely to happen are solved, and moreover renewable energy sources can be recycled, the substrate utilization rate is high, and the energy production efficiency is high. The method is mainly applied to the field of biological energy.

The invention discloses a method for enzymatic synthesis of theaflavin TF by virtue of tea polyphenol oxidase isoenzyme PPO1. The method is the optimization of a technique and a technology, which is capable of achieving the oriented enzymatic synthesis of a single theaflavin component TF through an enzymatic reaction with a shaking table as well as subsequent steps of extracting, centrifuging, concentrating, freeze-drying and the like by taking tea polyphenol oxidase isoenzyme PPO1 as an enzyme source, catechins EC and EGC components in tea polyphenol a major reaction substrates and a buffer solution at a certain pH value as a reaction system. The method overcomes shortcomings that multiple theaflavin components are generated simultaneously, a technology for separating and purifying the theaflavin components is complex, the preparation of a high-purity theaflavin component is quite difficult and the like in a conventional process which carries out the enzymatic synthesis of theaflavin by virtue of crude enzyme fluid of tea polyphenol oxidase; the method achieves a technological innovation that a single product is generated from enzymatic synthesis and a side product is avoided at reaction end, and the method avoids a technological bottleneck in subsequent separation and purification of the theaflavin component, so as to lay the theoretical and practical foundation for the green, safe and efficient large-scale industrial production and utilization of the theaflavin component.

The invention discloses a synthesis method of an antioxidant 1790 and belongs to chemical field. By adoption of the synthesis method, the problems of serious pollution, low raw material utilization ratio and high cost in the existing method are solved. The synthesis method is implemented by three steps of: (1) preparation of 4-tert-butyl-3-hydroxyl-2,6-dimethyl benzyl alcohol, wherein 2,4-dimethyl-6-tert-butyl phenol, concentrated hydrochloric acid and paraformaldehyde are stirred at the temperature of 40 DEG C and return for 50 hours to obtain 4-tert-butyl-3-hydroxyl-2,6-dimethyl benzyl alcohol, and the product does not need to be purified; (2) preparation of 4-tert-butyl-3-hydroxyl-2,6-dimethyl benzyl chloride, wherein hydroxyl groups of the 4-tert-butyl-3-hydroxyl-2,6-dimethyl benzyl alcohol are chloro-substituted by thionyl chloride to obtain the 4-tert-butyl-3-hydroxyl-2,6-dimethyl benzyl chloride, the product can be obtained by crystallization of methyl benzene and methyl alcohol, and after removal of a solvent, the crystallized mother liquor can be continuously used as a raw material; and (3) preparation of a 1790 product. The synthesis method disclosed by the invention has the advantages that the production cost is effectively reduced, the steps are simple and easy to operate, and the generated waste water is less, so that the industrial production is easy.

The invention discloses a method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and an application. The method includes the steps: inoculating thermophilic sucroclastic anaerobic bacillus seed solution into a fermentation culture medium; performing anaerobic fermentation to obtain the hydrogen. The fermentation culture medium is a culture medium taking the non-detoxified acid pretreatment lignocellulose as a carbon source. According to the method, two-stage fermentation is performed, or fermentation is performed after part of organic acid is additionally added. The yield of the hydrogen can reach 277.44mM (6.21L-H2/L) by the aid of the two-stage fermentation method, the yield of the hydrogen can be improved by 63.9% by adding 10g/L acetic acid, and the biological hydrogen production efficiency of bagasse can be remarkably improved. The method is suitable for transformation of the bagasse through microorganisms.

The invention belongs to the technical field of bioengineering and relates to a method for reducing decomposition of cephalosporin C (CPC). The method comprises the following steps: knocking out beta-lactamase genes and acetyl esterase genes of host bacteria by using a Red recombinant system; transforming cephalosporin C acylase genes into the host bacteria; expressing the cephalosporin C acylaseby using the host bacteria; preparing cephalosporin C acylase crude enzyme solution or immobilized cephalosporin C acylase crude enzyme; and adding the cephalosporin C acylase crude enzyme solution or immobilized cephalosporin C acylase crude enzyme into cephalosporin C substrate working liquid and catalyzing to generate 7-aminocephalosporin acid (ACA). CPC acylase is prepared by the method. In the process of catalyzing the CPC to generate 7-ACA by using the harvested crude enzyme, the utilization ratio of the CPC is increased to over 98 percent.

The invention relates to the technical field of displaying, and discloses an electronic paper display screen, display equipment and a binding method. The technical purpose is to increase the substrateutilization rate of the electronic paper display screen, increase the yield of the electronic paper display screen and prolong the service life of the electronic paper display screen. The electronicpaper display screen comprises a substrate, electronic paper, a binding electrode, an electronic ink conduction structure and chip-on-film, wherein the substrate comprises a displaying area and a binding area, the electronic paper is arranged at one side of the displaying area of the substrate, and the electronic ink conduction structure which extends to the substrate binding area is arranged at the edge, close to one side of the binding area, of the electronic paper; the chip-on-film is electrically connected with the substrate through the binding electrode, and the end, close to the displaying area, of the chip-on-film is clamped between the substrate and the electronic ink conduction structure.

The invention provides a method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina. The method comprises the steps of culturing the R. salina by using a seed culture medium; inoculating an artificial seawater fermentation medium with the R. salina; culturing the R. salina by using light with different wave lengths and intensities. The method for producing the phycoerythrin and the polyunsaturated fatty acid by using the R. salina increases the substrate utilization rate, and improves the biomass as well as the fermentation level of total protein, the phycoerythrin, lipid and the polyunsaturated fatty acid, thus being very beneficial to promotion of industrial development of producing the phycoerythrin and the polyunsaturated fatty acid by fermenting the R. salina.

The invention relates to a method of producing welan gum by utilization of alcaligenes fermentation and then by extraction with acetone. The method comprises S1. a step of preparing a strain preservation slant, wherein the alcaligenes ATCC31555 is inoculated onto a culture medium slant and cultured at a maintained temperature of 30 DEG C for 2-4 days and reserved for further use; S2. a step of picking a loop of the strain on the slant to a primary liquid seed culture medium by using an inoculating loop and culturing at 30 DEG C for 24 h to obtain primary liquid seeds; S3. a step of inoculating the primary liquid seeds to a secondary liquid seed culture medium according to an inoculation amount of 10%, and culturing at 28-38 DEG C for 12-24 h to obtain secondary liquid seeds; S3. a step of inoculating the secondary liquid seeds to a sterilized fermentation tank having a volume of 5 L and fermenting; S4. a step of discharging 1-3.5 L of fermentation liquid after fermentation is performed for 20-36 h, replenishing 1-3.5 L of a liquid fermentation culture medium, repeating for 2-3 times, and stopping the fermentation; and S5. a step of performing post-extraction of the fermentation liquid. The method shortens the fermentation period and increases the production efficiency and the yield.

A chip packaging method begins by fixing a chip to the top side of a substrate. The chip is then encapsulated in an encapsulant. After that, the encapsulant is drilled from its top side in order to have a through hole adjacent to the chip. Lastly, an area extending between the chip and the through hole and the hole wall of the through hole are plated with an electrically conductive metal to enable electrical connection between the chip and the substrate through the electrically conductive metal. The chip packaging method solves the problems of the conventional wire bonding method, simplifies the packaging process, and provides the packaged chips with high transmission efficiency.

The invention discloses a co-production method for alpha-arbutin with high optical purity and glucan with high adhesion by using leuconostoc pseudomesenteroides. The method takes sucrose and hydroquinone as substrates to produce the alpha-arbutin and the glucan by fermenting in one step under the weak acid environment, and comprises the following steps: 1) first level seed culture: inoculating a leuconostoc pseudomesenteroides solution in a seed medium with 0.1 to 0.2% inoculation amount, and cultivating at 37 DEG C and 250rpm for 10 to 12 hours; 2) second level seed culture: transmitting a first level seed solution to the seed medium with 1 to 2% inoculation amount, and cultivating at 37 DEG C and 250rpm for 10 to 12 hours; 3) fermentation culture: inoculating a second level seed solution in a fermentation medium with 5 to 8% inoculation amount, adjusting pH in a fermentation process to be 6.0 to 6.8, blowing air in the whole fermentation process, controlling the air flow to be 0.1 to 0. 3vvm, and cultivating at 37 DEG C and 250 to 300rpm for 12 to 16 hours. According to the method, the alpha-arbutin with high optical purity and the glucan with high adhesion can be directly obtained by the fermentation of bacterial strain, and the method is high in substrate use rate, simple and quick in reaction, mild in condition, low in energy consumption, and suitable for industrial production.

The invention discloses a high-viscosity micro-molecular-weight dextran production method. The high-viscosity micro-molecular-weight dextran production method comprises the steps that leuconostoc mesenteroides is utilized and gluco disaccharide is used as a substrate, and fermentation production is performed in an alkaline range to obtain high-viscosity micro-molecular-weight dextran. The leuconostoc mesenteroides is leuconostoc mesenteroides G123, and a preservation number is CCTCC NO: M2014115. The dextran is at least one of maltose, cellobiose, trehalose, kojibiose or gentiobiose. The alkaline range is pH 7.0-8.0. The viscosity of the high-viscosity micro-molecular-weight dextran is not lower than 5800 cP, and the molecular weight ranges from 1000 Da to 2500 Da. The micro-molecular-weight dextran is high in viscosity, is excellent microbial metabolism glue and has wide application prospect. The production method is mild in reaction condition, low in energy consumption, high in yield and applicable to industrial large-scale production.