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Method of producing welan gum by tank-dividing method fermentation

A technology of Welan gum and yeast powder, which is applied in the field of Welan gum fermented by cutting tank method, can solve the problems of high extraction cost, inability to form industrialized production, and poor quality of Welan gum finished products, and achieve obvious decolorization effect , The market advantage is obvious, and the effect of reducing cumbersome

Inactive Publication Date: 2014-04-02
天津科百生物科技研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

unable to form industrialized production
Mainly because the strains used for fermentation have high requirements on raw materials and the yield is too low, resulting in a low concentration of Welan gum in the fermentation broth, and there are too many by-products in the fermentation broth
These factors further cause the cost of extraction to remain high. On the other hand, the quality of domestic Welan gum products is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] S1. Preparation of strain preservation slope: Alcaligenes sp. ATCC31555 was inoculated on the culture slope, and incubated at 30°C for 2-4 days for later use; the composition of the slope culture medium included 1L unit: 15g sucrose, Yeast powder 3g, peptone 5g, beef extract 5g, agar 15g, distilled water to a volume of 1L, pH 7.0; the strain used: Alcaligenes sp. ATCC31555;

[0018] S2. The above-mentioned slope is used to inoculate the sterilized first-level liquid seed culture medium. The composition of the first-level liquid seed culture medium includes 1L: glucose 15g, yeast powder 4g, beef extract 4g, peptone 3g, K 2 HPO 4 2g, MgSO 4 0.01g, distilled water to a constant volume of 1L, pH 7.0; sterilize the liquid seed culture medium at 115°C for 15 minutes and then cool; then use an inoculation loop to pick a loop of bacteria from the strain preservation slope and connect it to the above-mentioned cooled liquid seed In the medium, the volume of the 500ml shake flask i...

Embodiment 2

[0025] S1. Preparation of strain preservation slope: Alcaligenes sp. ATCC31555 was inoculated on the culture slope, and incubated at 30°C for 2-4 days for later use; the composition of the slope culture medium included 1L unit: 15g sucrose, Yeast powder 3g, peptone 5g, beef extract 5g, agar 15g, distilled water to a volume of 1L, pH 7.0; the strain used: Alcaligenes sp. ATCC31555:

[0026] S2. The above-mentioned slope is used to inoculate the sterilized first-level liquid seed culture medium. The composition of the first-level liquid seed culture medium includes 1L unit: 20g glucose, 8g yeast powder, 8g beef extract, 6g peptone, K 2 HPO 4 3g, MgSO 4 0.03g, distilled water to a constant volume of 1L, pH 7.0; sterilize the liquid seed culture medium at 115°C for 15 minutes and then cool; then use an inoculation loop to pick a loop of bacteria from the culture preservation slope and connect to the above-mentioned cooled liquid seed In the medium, the volume of the 500ml shake flas...

Embodiment 3

[0033] S1. Preparation of strain preservation slope: Alcaligenes sp. ATCC31555 was inoculated on the culture slope, and incubated at 30°C for 2-4 days for later use; the composition of the slope culture medium included 1L unit: 15g sucrose, Yeast powder 3g, peptone 5g, beef extract 5g, agar 15g, distilled water to a volume of 1L, pH 7.0; the strain used: Alcaligenes sp. ATCC31555;

[0034] S2. The above-mentioned slope is used to inoculate the sterilized first-level liquid seed culture medium. The composition of the first-level liquid seed culture medium includes 1L: glucose 18g, yeast powder 5g, beef extract 6g, peptone 4g, K 2 HPO 4 2.5g, MgSO 4 0.02g, distilled water to a constant volume of 1L, pH 7.0; sterilize the liquid seed culture medium at 115°C for 15 minutes and then cool; then use an inoculation loop to pick a loop of bacteria from the culture preservation slope and connect it to the above-mentioned cooled liquid seed In the medium, the volume of the 500ml shake flas...

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Abstract

The invention relates to a method of producing welan gum by utilization of alcaligenes fermentation and then by extraction with acetone. The method comprises S1. a step of preparing a strain preservation slant, wherein the alcaligenes ATCC31555 is inoculated onto a culture medium slant and cultured at a maintained temperature of 30 DEG C for 2-4 days and reserved for further use; S2. a step of picking a loop of the strain on the slant to a primary liquid seed culture medium by using an inoculating loop and culturing at 30 DEG C for 24 h to obtain primary liquid seeds; S3. a step of inoculating the primary liquid seeds to a secondary liquid seed culture medium according to an inoculation amount of 10%, and culturing at 28-38 DEG C for 12-24 h to obtain secondary liquid seeds; S3. a step of inoculating the secondary liquid seeds to a sterilized fermentation tank having a volume of 5 L and fermenting; S4. a step of discharging 1-3.5 L of fermentation liquid after fermentation is performed for 20-36 h, replenishing 1-3.5 L of a liquid fermentation culture medium, repeating for 2-3 times, and stopping the fermentation; and S5. a step of performing post-extraction of the fermentation liquid. The method shortens the fermentation period and increases the production efficiency and the yield.

Description

Technical field [0001] The invention relates to a method for fermenting welan gum by using Alcaligenes bacillus, in particular to a method for fermenting and producing welan gum by cutting tank method and extracting with acetone. Background technique [0002] Microbial metabolism glue is also called biosynthetic glue. Many microorganisms can produce a certain amount of various polysaccharides under different external conditions during growth and metabolism. Microbial polysaccharides are safe and non-toxic, with unique physical and chemical properties. Compared with plant and animal polysaccharides, the production of microbial polysaccharides is less affected by factors such as geographical environment, climate, and natural disasters, and the yield and quality are stable. Welan gum is a new type of microbial polysaccharide. The structural skeleton of Welan gum is composed of D-glucose, D-glucuronic acid, D-glucose and L-rhamnose units. Welan gum contains 2.8%-7.5% acetyl groups,...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/05
Inventor 李红芬梁军军刘云
Owner 天津科百生物科技研发有限公司
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