Recombinant bacteria capable of synthesizing 3-hydracrylic acid by using glucan as well as construction method and application of recombinant bacteria

A technology of hydroxypropionic acid and recombinant bacteria, applied in the field of genetic engineering, can solve the problems such as limited utilization rate of 3-hydroxypropionic acid substrate and limited yield, and achieve the effect of improving the utilization rate of substrate and improving yield
CN107119003AActive Publication Date: 2017-09-01QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2017-09-01

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Abstract

The invention provides recombinant bacteria capable of synthesizing 3-hydracrylic acid by using glucan as well as a construction method and application of the recombinant bacteria, and belongs to the field of gene engineering and fermentation engineering. Klebsiella pneumoniae is host bacteria for expressing a gene lgk capable of encoding levodextran kinase and a gene aldH capable of encoding aldehyde dehydrogenase. The construction method comprises the following steps: obtaining a recombinant carrier puC18-lgk-aldH, converting the recombinant carrier puC18-lgk-aldH into a competent cell of the host Klebsiella pneumoniae, and obtaining the recombinant bacteria. By use of the levodextran kinase gene lgk and the aldehyde dehydrogenase gene aldH which are optimized through a codon, in combination with the whole scheme, the substrate use rate is increased, and the yield is increased.
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Description

technical field

[0001] The invention relates to a recombinant bacterium for synthesizing 3-hydroxypropionic acid by using dextran, its construction method and application, and belongs to the technical field of genetic engineering. Background technique

[0002] Energy is the basis for the survival and development of modern society. With the development of science and technology, the demand and dependence on energy are also increasing. However, due to the non-renewability of fossil energy, which accounts for 87.7% of the world's primary energy, the fact of energy shortage has become a serious problem before people. The increasing shortage of energy has made the focus of the world's attention on the development and utilization of new energy that can replace fossil energy. Biomass is the only renewable resource that can be converted into liquid fuels. Biomass energy resources are very rich, and the amount of newly generated biomass resources in the world can reach 170 billion ...

Claims

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