Method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and application

A technology for lignocellulose and acid pretreatment, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing production costs, good applicability, and simplifying operating processes

Inactive Publication Date: 2017-09-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expected cost of the process is low and the treatment cost is also low. The fermentation process is simple and the hydrogen output is high. At the same time, the environmental pollution caused by pretreatment is reduced, and the cost of raw materials and pretreatment is greatly saved. The utilization rate of the substrate solves the problem of low efficiency of lignocellulose in the biohydrogen production process, and has high economic feasibility

Method used

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  • Method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and application
  • Method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and application
  • Method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Hydrogen production capacity of different concentrations of substrates under simultaneous saccharification and fermentation mode

[0069] (1) Preparation of the anaerobic Bacillus thermosaccharolyticum seed solution: The anaerobic Bacillus thermosaccharolyticus MJ1 stored at -80°C was cultured by shaking at 55°C and 150rpm in a 10mL vial (containing 4.5mL seed medium). Activation was carried out for 18 hours, followed by shaking culture in a 100 ml serum bottle (containing 45 mL seed medium) at 55° C. and 150 rpm for 18 hours for amplification to obtain seed liquid. Wherein the seed medium is a xylose liquid medium containing 5g / L xylose, and the main components of the medium are: xylose 5g / L (carbon source), ammonium chloride 1g / L, sodium chloride 1g / L, hydrogen phosphate Dipotassium 1g / L, potassium dihydrogen phosphate 1g / L, cysteine ​​0.5g / L, magnesium chloride hexahydrate 0.5g / L, potassium chloride 0.2g / L, yeast powder 2g / L, peptone 2g / L, Trace element s...

Embodiment 2

[0077] Example 2: Hydrogen production capacity of different concentrations of substrates under pre-hydrolysis synchronous saccharification and fermentation mode

[0078] (1) The preparation of the seed solution is the same as in Example 1.

[0079] (2) The preparation and sterilization of the fermentation medium are the same as in Example 1.

[0080] (3) Hydrogen production in the first stage under the prehydrolysis and synchronous saccharification and fermentation mode of thermophilic anaerobic saccharolytica: inject cellulase CTec2 into the medium with a syringe at 20 U / g bagasse, and enzymatically hydrolyze at 50°C for 72 hours Then the prepared seed solution was injected into the above-mentioned fermentation medium after enzymolysis according to the inoculation amount of 10% (v / v), the fermentation temperature was 55° C., the shaking table speed was 150 rpm, and the fermentation was terminated after 120 hours of fermentation. The result is as figure 2 Shown, the output ...

Embodiment 3

[0081] Embodiment 3: The method for carrying out the second-stage fermentation hydrogen production under different fermentation modes

[0082] (1) The preparation of the seed solution is the same as in Example 1.

[0083] (2) The preparation and sterilization of the fermentation medium are the same as in Example 1.

[0084] (3) The hydrogen production in the first stage under the synchronous saccharification and fermentation mode of thermophilic saccharolytic anaerobic bacteria is the same as in Example 1.

[0085] (4) The hydrogen production in the first stage under the prehydrolysis and synchronous saccharification and fermentation mode of thermophilic saccharolytica anaerobic bacteria is the same as in Example 2.

[0086] (5) Hydrogen production in the second stage of fermentation under different fermentation modes: after the completion of the first stage of fermentation, adjust the pH value of the fermentation medium (100g / L bagasse) to 7.0 after the simultaneous sacchari...

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Abstract

The invention discloses a method for generating hydrogen directly by non-detoxified acid pretreatment lignocellulose and an application. The method includes the steps: inoculating thermophilic sucroclastic anaerobic bacillus seed solution into a fermentation culture medium; performing anaerobic fermentation to obtain the hydrogen. The fermentation culture medium is a culture medium taking the non-detoxified acid pretreatment lignocellulose as a carbon source. According to the method, two-stage fermentation is performed, or fermentation is performed after part of organic acid is additionally added. The yield of the hydrogen can reach 277.44mM (6.21L-H2/L) by the aid of the two-stage fermentation method, the yield of the hydrogen can be improved by 63.9% by adding 10g/L acetic acid, and the biological hydrogen production efficiency of bagasse can be remarkably improved. The method is suitable for transformation of the bagasse through microorganisms.

Description

technical field [0001] The invention belongs to the technical field of hydrogen production by microbial fermentation, and in particular relates to a hydrogen production method and application of directly using undetoxified acid pretreated lignocellulose. Background technique [0002] As mankind enters the 21st century, the rapid development of various technologies has led to the massive consumption of fossil fuels, such as coal, oil, and natural gas, which provide about 95% of the world's needs. With the further development of society, the demand for energy will further expand. The massive consumption of fossil fuels has also caused serious environmental pollution problems, such as the current smog problem, which has caused people to turn pale when talking about smog. What is more important is the non-renewability of fossil fuels. The depletion of energy will affect the stability of society. The search for an alternative energy source that is renewable, environmentally frie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
CPCC12P3/00C12P2201/00
Inventor 朱明军胡彬彬
Owner SOUTH CHINA UNIV OF TECH
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