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Process for preparing alcohol through utilizing xylose and glucose by microzyme

A yeast and glucose technology, applied in fermentation, biofuel, etc., can solve the problems of long fermentation cycle and achieve the effects of short cycle, high substrate conversion rate and high alcohol yield

Inactive Publication Date: 2005-06-22
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yeasts that can simultaneously metabolize xylose and glucose into alcohol include Pachysomyces tannophilus, Candida shohata, Pichia stipitis, Kluyveromyces marxianus and Candida sp XF217) etc., but they utilize xylose and glucose to ferment and produce the actual yield of alcohol only about 70% of the theoretical yield, and the fermentation period is long, about 80 hours

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Pachysomyces tannophilus 2.1662 Method for the production of alcohol using xylose and glucose.

[0026] 100ml of acclimatization medium is housed in a 250ml Erlenmeyer flask, the formula of the acclimatization medium is: xylose 20g / L, peptone 5g / L, yeast juice 3g / L. The yeast Pachysolen tannophilus 2.1662 preserved on the slant was inoculated in the acclimatization medium, and the inoculum amount was 2%. The culture was carried out for 48 hours under the conditions of pH 5.0, 30° C., and 60 rpm, and a total of 7 batches of acclimatization culture were carried out. The number of bacteria in the culture medium was counted by direct microscope counting method, and the number of bacteria increased significantly with the increase of domestication batches. 3.3×10 8 / ml, and the bacterial content of this strain after the first domestication was only 5.0×10 7 / ml, the bacteria content increased by 1 order of magnitude after the first domestication. The increase of bacteria ...

Embodiment 2

[0033] Process for the production of alcohol from xylose and glucose by Pachysomyces tannophilus ATCC 32691.

[0034] 100ml of acclimatization medium is housed in a 250ml Erlenmeyer flask, and the formulation of the acclimatization medium is: xylose 10g / L, peptone 4g / L, yeast juice 2g / L. The yeast Pachysolen tannophilus ATCC32691 preserved on the slant was inoculated in the acclimatization medium, and the inoculum amount was 3%. The culture was carried out for 60 hours under the conditions of pH 5.5, 35° C., and 80 rpm, and a total of 6 batches of acclimatization culture were carried out. The bacteria content in the culture solution was counted by microscope direct counting method, and the bacteria content increased obviously with the increase of domestication batches. The increase of bacteria content indicated that the ability of metabolizing xylose and glucose was improved.

[0035] The domesticated yeast Pachysolen tannophilus ATCC 32691 was cultured in liquid. 100ml of ...

Embodiment 3

[0041] Candida shohata ATCC 34887 Alcohol production from xylose and glucose.

[0042] 100ml of acclimatization medium is housed in a 250ml Erlenmeyer flask, the formulation of the acclimatization medium is: xylose 50g / L, peptone 6g / L, yeast juice 4g / L. The yeast Candida shohata ATCC34887 preserved on the slant was inoculated in the acclimatization medium, and the inoculum amount was 5%. The culture was carried out for 96 hours under the conditions of pH 5.5, 28° C., and 120 rpm, and a total of 5 batches of acclimatization culture were carried out. The bacteria content in the culture solution was counted by microscope direct counting method, and the bacteria content increased obviously with the increase of domestication batches. The increase of bacteria content indicated that the ability of metabolizing xylose and glucose was improved.

[0043] The domesticated yeast Candida shohata ATCC 34887 was cultured in liquid. 100ml of liquid medium is housed in a 250ml Erlenmeyer fl...

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Abstract

The invention provides a process for preparing alcohol through utilizing xylose and glucose by microzyme, in particular a process for preparing alcohol by using one of Pachysolen tannophilus 2.1662, Pachysolen tannophilus 32691, Candida shehatae ATCC 34887, xylose and glucose as substrate, the preparation comprises the steps of domestication, fluid culture, immobilization, enrichment culture. The invention can realize shortened production cycle and increased substrate output coefficient.

Description

technical field [0001] The present invention relates to the application of yeast, in particular to the use of one of three yeast strains Pachysolen tannophilus (Pachysolen tannophilus) 2.1662, Pachysolen tannophilus ATCC 32691, and Candida shehatae (Candida shehatae) ATCC 34887 Alcohol production from xylose and glucose. Background technique [0002] Alcohol, also known as ethanol, is usually produced by fermentation of six-carbon sugars by Saccharomyces cerevisiae. The production of fuel ethanol from biomass (crop stalks, wood processing waste, etc.) is an important part of the national energy strategy and national energy security, and it is also an important way to prevent and reduce air pollution caused by vehicle combustion exhaust. An important guarantee for sustainable development. In biomass, cellulose and hemicellulose are the main chemical components. Hemicellulose includes various polypentoses and polyhexoses. The most common hemicellulose is xylan, which account...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06
CPCY02E50/10
Inventor 杨秀山田沈
Owner CAPITAL NORMAL UNIVERSITY
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