A kind of Sclerotinum and method for fermenting and producing scleroglucan

A technology of Sclerotinia and dextran, applied in the field of microorganisms, can solve problems such as high production costs, waste of resources, and troublesome follow-up processing of products, and achieve the effect of increasing substrate utilization and reducing costs significantly

Active Publication Date: 2019-11-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main carbon source for scleroglucan production is sucrose. Although the output reaches 26g / L, the substrate utilization rate is low, the production cost is high, and resources are seriously wasted.
The use of corn starch and corn yellow pulp to produce scleroglucan is not suitable for all regions, and the subsequent processing of the product is troublesome

Method used

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  • A kind of Sclerotinum and method for fermenting and producing scleroglucan
  • A kind of Sclerotinum and method for fermenting and producing scleroglucan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The isolation and identification of embodiment 1 bacterial strain

[0024] The strains isolated earlier were inoculated on PDA medium, the genomic DNA of the strains was extracted, and the gene sequence of 18S rDNA was amplified (the universal primers for PCR amplification were NS1F and NS8R). The PCR product was identified by 1% agarose gel electrophoresis, and the result was good. The PCR amplification product was sent to Sangon Bioengineering (Shanghai) Co., Ltd. for sequencing, and the sequencing results were compared with BLAST sequences on the NCBI website, and it was determined to be Sclerotium rolfsii.

[0025] The key substantive difference between the Sclerotinia provided by the present invention and the existing Sclerotinia: Compared with the existing Sclerotinia, the Sclerotinia provided by the present invention is easier to use glucose to produce scleroglucan. In the culture medium of glucose and glucose, the yield in the glucose medium is obviously better...

Embodiment 2

[0026] The cultivation of embodiment 2 Sclerotin and the fermentation of scleroglucan

[0027] Fermentation medium (g / L): KH 2 PO 4 1.0; KCL 0.5; citric acid 1.5; MgSO 4 ·7H 2 O 0.5g / L; pH4.0; add different amounts of glucose (45, 50, 55), NaNO 3 (2, 2.25, 2.5), yeast extract powder (0.2, 0.37, 0.5).

[0028] Shake flask culture: inoculate an appropriate amount of mycelia into a 250ml shake flask containing 50ml of seed medium, culture at 28°C and 220r / min for 72h, and transfer 5% of the inoculum to a 25ml glucose fermentation medium. In a 250ml shake flask, culture at 30°C and 220r / min for 96h.

[0029] Adjust the part components (as shown in Table 1) of fermentation medium respectively, all the other components remain unchanged:

[0030] Table 1 Composition adjustment and corresponding yield of fermentation medium

[0031] serial number Glucose (g / L) NaNO 3 (g / L)

[0032] The results show that the culture medium formula (g / L): glucose 55; NaNO 3 2.2...

Embodiment 3

[0033] The impact of embodiment 3 different carbon source concentrations on the production of scleroglucan

[0034] The specific embodiment is the same as Example 2, the difference is that the fermentation medium formula is: sodium nitrate 2.2g / L, yeast extract 0.5g / L, KH2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, citric acid 1.5g / L, KCl 0.5g / L, pH 4.5; different glucose additions (55, 75, 95g / L) were used to investigate the effect of carbon source on the production of scleroglucan.

[0035] Batch culture in a 3L fermenter: Transfer the above seed solution cultured at 28°C and 220r / min for 72h to a 500ml shake flask with 100ml of seed solution at a 5% inoculum size, and transfer the seed solution at 28°C and 220r / min Cultivate under conditions for 48 hours, and then inoculate 5% inoculum in a 3L fermenter with 1.5L fermentation medium for 96 hours, set the temperature at 30°C, and stir at a speed of 350r / min.

[0036] Fermentation results such as figure 2 As shown, the effect ...

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Abstract

The invention discloses a Sclerotinum and a method for fermenting and producing scleroglucan, belonging to the technical field of microbes. The strain CCTCC NO:M2017646 provided by the present invention can be fermented for 56 hours under the condition of glucose carbon source concentration of 75g / L to reach the effect of scleroglucan production of nearly 20g / L, which is obviously better than the current international scleroglucan production of 150g / L. Under the culture condition of L sucrose, the yield of 72h is 26g / L, the substrate utilization rate is increased by 58%, and the cost is significantly reduced. The method is superior to the existing dextran production process and has important industrial application prospects.

Description

technical field [0001] The invention relates to a sclerotinum and a method for fermenting and producing scleroglucan, belonging to the technical field of microorganisms. Background technique [0002] Scleroglucan, also known as Sclerotin, is a high molecular weight, non-ionic branched glucan. It is a dextran with β-D-(1-3) glucopyranosyl as the main chain, and every three glucose is β-D-(1-6) glucopyranosyl as the side chain. Scleroglucan can be obtained by pure culture and fermentation of filamentous fungi of the genus Sclerotinia. [0003] Scleroglucan has high temperature resistance, various electrolytes (5% NaCl, 5% Na 2 SO 4 , 20% CaCl 2 etc.) and a wide range of pH and other characteristics. It is widely used as a thickener, gel or stabilizer in the food industry, and as a tablet coating, eye drops, etc. in the pharmaceutical industry. [0004] At present, microorganisms have been used to produce scleroglucan. However, because scleroglucan is usually fermented by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/08A23L29/269A61K47/36C12R1/645
CPCA23V2002/00A61K47/36A23L29/269C12P19/08C12N1/145C12R2001/645A23V2200/242A23V2200/228A23V2250/5042
Inventor 周景文陈坚曾伟主高荣伟堵国成
Owner JIANGNAN UNIV
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