Method for producing L-diaminocaproic acid through multistage continuous fermentation

A lysine and fermenter technology, applied in the field of L-lysine preparation, can solve the problems of reducing the utilization rate of the product, affecting the yield of the product, etc., and achieve the advantages of high utilization rate of the substrate, stable yield and lower production cost. Effect
CN106148444AInactive Publication Date: 2016-11-23NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2016-11-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for producing L- diaminocaproic acid through multistage continuous fermentation. The method includes: starting from escherichia coil to obtain an L-diaminocaproic product through fermentation above stage four; specifically stringing at least four fermentation tanks integrally, and controlling carbon source concentration and nitrogen source concentration in the fermentation tanks of different stages by regulating ventilatory capacity, initial sugar concentration of the primary fermentation tank, and addition rates, fermented-liquid outflowing rate and fermented liquid inflowing rate of carbon source filling containers and nitrogen source filling containers of the fermentation tanks; controlling the fermentation tanks of different stages to enable the specific growth rate of thallus of the fermentation tanks of the different stages to be 0.3-0.8h<1> to obtain the fermentation product. With the method, high thalli concentration in the fermentation system is maintained, lag phase of cells is shortened, substrate utilization is higher as compared with batch fermentation, target product quantity is high, yield is stable, operations of repeated tank washing, sterilizing, inoculating and the like are not needed, and production cost is lowered.
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Description

technical field

[0001] The invention belongs to the field of L-lysine preparation, and in particular relates to a method for producing L-lysine by multistage continuous fermentation. Background technique

[0002] The chemical name of lysine is 2,6-diaminocaproic acid (2,6- diaminohexanoic acid ), molecular formula NH 2 (CH 2 ) 4 CH. (NH 2 )COOH, with a molecular weight of 146.19, is one of the nine essential amino acids for living organisms. There are two types of lysine in nature: L-type and D-type, and L-lysine can be used by humans and animals. Lysine can regulate the balance of metabolism in the body, improve the body's absorption of cereal protein, improve human dietary nutrition and animal nutrition, and promote growth and development; it can also promote the secretion of digestive enzymes, promote the delivery of intracellular calcium, and improve the metabolism of nitrogen in the body balance.

[0003] Lysine is the second most productive commercial amino acid...

Claims

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