Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system

A bioreactor and fiber bed technology, applied in the field of bioengineering, can solve the problems of difficult industrial scale-up of immobilized fermentation reactors, bioreactor system scale-up, low fermentation efficiency, etc., to achieve large-scale industrial scale-up and shorten delay period, the effect of increasing the concentration of the final product

Inactive Publication Date: 2013-09-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems of low fermentation efficiency, low yield and yield caused by product inhibition and low cell density in free fermentation, and the difficulty of industrial scale-up of common immobilized fermentation reactors, the purpose of the present invention is to provide a production process that can greatly Shorten the production cycle, increase the concentration of the final product, facilitate the subsequent separation of the fermentation broth, and effectively solve the problem of bioreactor system amplification. It is a method for producing butyric acid by fermentation in a multi-fiber bed bioreactor system suitable for industrial production.

Method used

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  • Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system
  • Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system
  • Method for producing butyric acid through fermentation of multilinked fiber bed bioreactor system

Examples

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Effect test

Embodiment 1

[0023] What this example provides is the process of producing butyric acid by fermentation of two fiber bed bioreactors in series, and the steps are as follows:

[0024] (1) Preparation of multi-fiber bed bioreactor: figure 1 The multi-connected fiber bed bioreactor is a fiber bed bioreactor formed by two fiber bed bioreactors 2 connected in series; a pump 3 and a flowmeter 4 are set on the material inlet and material outlet pipelines of each fiber bed bioreactor, A pH meter 7 and a thermometer 5 are installed in the outlet pipeline of each fiber bed bioreactor. The fermentation state of each fiber bed bioreactor is detected by detection elements such as flow meters, pH meters, and thermometers, and the circulation speed of the fermentation broth is controlled by pumps. Each fiber bed bioreactor is an interlayer packed column composed of concentric inner and outer cylinders. In this example, the volume of each fiber bed filled column is 500mL, and the packed column is made o...

Embodiment 2

[0029] What this example provides is the technology that produces butyric acid by fermentation of two fiber bed bioreactors in parallel system, and the steps are as follows:

[0030]1) The preparation of the multi-fiber bed bioreactor is the same as in Example 1, the difference is that the multi-fiber bed bioreactor is a fiber bed bioreactor formed by two fiber bed bioreactors 2 connected in parallel; each fiber bed is filled with a column The volume is 500mL, and the filled cylinder is made of glass;

[0031] 2) same as embodiment 1 step 2);

[0032] 3) Same as step 3 of embodiment 1);

[0033] 4) Same as Step 4) of Example 1, using batch fermentation, the final concentration of butyric acid in the fermentation broth was 19.44g / L.

[0034] In this example, when the sugar concentration in the fermentation broth is close to 0, glucose solution is added for fed-batch fermentation, and the final concentration of butyric acid in the fermentation broth is 74.72g / L. The fermentat...

Embodiment 3

[0036] What this example provides is the technology that produces butyric acid by the fermentation of three fiber bed bioreactors in parallel system, and the steps are as follows:

[0037] 1) The preparation of the multi-fiber bed bioreactor is the same as in Example 1, the difference is that the multi-fiber bed bioreactor is a fiber bed bioreactor formed by three fiber bed bioreactors 2 in parallel; each fiber bed is filled with a column The volume is 500mL, and the filled cylinder is made of glass;

[0038] 2) same as embodiment 1 step 2);

[0039] 3) Same as step 3 of embodiment 1);

[0040] 4) Same as step 4) of Example 1, using batch fermentation, the final concentration of butyric acid in the fermentation broth is 26.09g / L.

[0041] In this example, when the sugar concentration in the fermentation broth is close to 0, glucose solution is added to carry out fed-batch fermentation, and the final concentration of butyric acid in the fermentation broth is about 78g / L. The...

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Abstract

The invention discloses a method for producing butyric acid through fermentation of a multilinked fiber bed bioreactor system. The method comprises the following steps: preparing a multilinked fiber bed bioreactor, activating a strain, culturing a seed liquid, performing pre-fermentation culture, and performing pre-fermentation batch fermentation or fed-batch fermentation. The method is simple in process; through the adoption of the method, on the one hand, large-scale industrial amplification can be realized at a relatively lower cost; on the other hand, a multi-phase mass transfer problem caused by simple volume amplification of the reactor and insufficiency of filler strength of a fiber bed can be avoided; the lag phase of a fermentation process can be shortened; the production cycle is greatly shortened; the concentration of a final product is improved; the subsequent separation of a fermentation liquid is facilitated; and detection and control of the fermentation process and equipment maintenance are facilitated.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing butyric acid by fermentation using a multi-fiber bed bioreactor system. Background technique [0002] Butyric acid is a versatile fine chemical widely used in food, chemical and pharmaceutical industries. It is used in the preparation of candy and beverage flavoring agents in the food industry, and in the preparation of fruit flavors in the spice industry; cellulose butyrate is superior to cellulose acetate in terms of anti-aging, water resistance, and shrinkage; cellulose acetate butyrate can It is soluble in many solvents, and has better mixing ability with resins and plastics than cellulose monoacetate, and has good heat resistance, light resistance and moisture resistance; it can also be used in the preparation of pharmaceutical intermediates, such as γ-aminobutyric acid Remarkable anti-hypoxia ability, ethyl (R)-2-hydroxy-4-phenylbu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/52C12M1/00C12R1/145
Inventor 蔡谨徐志南黄磊王金施周铭
Owner ZHEJIANG UNIV
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