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Method for generating energy from hydrogen production bacteria and oily microalgae in stair-coupling mode

A technology of bacteria and microalgae, applied in the field of bioenergy, can solve the problems of difficult utilization of fermentation products, low substrate utilization rate, and low production efficiency, and achieve the goal of accelerating the pace of industrialization, high substrate utilization rate, and high production efficiency Effect

Inactive Publication Date: 2014-02-05
HARBIN INST OF TECH
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of difficult use of fermentation products, easy to cause environmental pollution, low substrate utilization rate and low production efficiency in the current dark fermentation hydrogen production, and to provide a cascade coupling production capacity of hydrogen-producing bacteria and oil-containing microalgae method

Method used

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  • Method for generating energy from hydrogen production bacteria and oily microalgae in stair-coupling mode
  • Method for generating energy from hydrogen production bacteria and oily microalgae in stair-coupling mode
  • Method for generating energy from hydrogen production bacteria and oily microalgae in stair-coupling mode

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specific Embodiment approach 1

[0021] Specific implementation mode 1: In this implementation mode, a method for coupling production capacity of hydrogen-producing bacteria and oil-containing microalgae is carried out according to the following steps:

[0022] 1. Take hydrogen-producing bacteria for cultivation;

[0023] 2. Centrifuge the organic acid fermentation liquid produced by the hydrogen-producing bacteria in step 1, take the supernatant to adjust the pH to 6-9, and then perform high-pressure steam sterilization;

[0024] 3. Inoculate oil-containing microalgae into the sterilized supernatant of step 2 for cultivation;

[0025] 4. Harvesting the oleaginous microalgae cultivated in step 3, and then extracting the oil in the oleaginous microalgae.

[0026] The beneficial effect of this implementation mode:

[0027] 1. The combination of dark fermentation hydrogen production technology and microalgae oil production technology can simultaneously solve the two bottleneck problems of raw material shortage...

specific Embodiment approach 2

[0031] Embodiment 2: This embodiment differs from Embodiment 1 in that the hydrogen-producing bacteria described in step 1 are Ethanoligenens harbinense, Clostridium pasteurianum, Escherichia coli ), Clostridium butyricum, Clostridium acetobutylicum, and Enterobacter aerogenes were mixed in any ratio. Others are the same as in the first embodiment.

[0032] The hydrogen-producing bacteria described in this embodiment are all purchased.

specific Embodiment approach 3

[0033] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 or 2 is that the hydrogen-producing bacteria culture conditions described in step 1 are at a temperature of 35°C to 37°C, protected from light, filled with nitrogen to isolate the air culture, and the culture time 20 to 40 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for generating energy from hydrogen production bacteria and oily microalgae in a stair-coupling mode, and relates to the field of biological energy, and mainly aims at solving the problems that the conventional fermentation products in dark fermentation hydrogen production are hard to utilize, environment pollution is easy to cause, the substrate utilization rate is low and the energy production efficiency is low. The method comprises the following steps: firstly, taking the hydrogen production bacteria to culture, secondly, centrifuging an organic acid fermentation liquid, thirdly, inoculating the oily microalgae, and fourthly, extracting grease. According to the method, a dark fermentation hydrogen production technique is combined with a microalgae oil production technique, the problems that the fermentation products in the dark fermentation hydrogen production are hard to utilize and environment pollution is likely to happen are solved, and moreover renewable energy sources can be recycled, the substrate utilization rate is high, and the energy production efficiency is high. The method is mainly applied to the field of biological energy.

Description

technical field [0001] The invention relates to the field of bioenergy. Background technique [0002] Population growth and economic development have brought about a huge demand for energy. However, traditional fossil energy (oil, coal, natural gas) is increasingly depleted, and it is difficult to meet the growing demand, and the use of fossil energy will bring about greenhouse effects and many other Environmental problems pose a serious threat to human survival and sustainable development. Therefore, the development of efficient, green and environmentally friendly renewable energy is imminent. The development and utilization of renewable energy such as biomass energy has become a hot spot of widespread concern around the world. Biodiesel and biohydrogen are new types of "green energy", which have dual functions of regeneration and environmental protection. [0003] It is in line with the strategic needs of the national "Twelfth Five-Year Plan" for energy conservation, em...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P3/00C12R1/145C12R1/19C12R1/89C12R1/01
CPCY02P20/10Y02P20/59
Inventor 任南琪任宏宇刘冰峰谢国俊赵磊孔凡英
Owner HARBIN INST OF TECH
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