Method of increasing oil yield and in-situ flocculation harvesting efficiency of microalgae growing in sewage

A technology of microalgae oil and in-situ flocculation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low oil content of growing microalgae, low recovery rate of microalgae, etc., and achieve the reduction of organic acid content, improve the water environment, and realize the effect of resource treatment

Pending Publication Date: 2019-12-31
HARBIN INST OF TECH
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of low oil content of growing microalgae in existing sewage and low recovery rate of microalgae, and provide a method for improving oil production of growing microalgae in sewage and in-situ flocculation recovery efficiency

Method used

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  • Method of increasing oil yield and in-situ flocculation harvesting efficiency of microalgae growing in sewage
  • Method of increasing oil yield and in-situ flocculation harvesting efficiency of microalgae growing in sewage
  • Method of increasing oil yield and in-situ flocculation harvesting efficiency of microalgae growing in sewage

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specific Embodiment approach 1

[0014] Specific Embodiment 1: A method of increasing the oil production of microalgae grown in sewage and the recovery efficiency of in-situ flocculation in this embodiment is achieved through the following steps:

[0015] 1. Add metal ions to the sewage and mix them evenly to obtain a mixed solution A; 2. Add the microalgae seed solution to the mixed solution A and ferment until the fermentation is stable; 3. After the microalgae fermentation is stable, discharge part of the microalgae Fermentation liquid, and add the same amount of mixed solution A, adjust the metal ion concentration to be the same as the metal ion concentration of the mixed solution A in step 1 by adding metal ions, continue to ferment after mixing evenly; 4, repeat step 3 0-100 times After the fermentation of step 3 is completed, or the operation of step 3 is omitted and the fermentation of step 2 is stable, the microalgae fermentation liquid is allowed to settle, and the flocs are settled to complete the h...

specific Embodiment approach 2

[0018] Embodiment 2: This embodiment differs from Embodiment 1 in that the sewage described in step 1 is one or more of fatty acid-containing sewage, protein sewage, carbohydrate sewage, and domestic sewage mixed in any ratio. Others are the same as the first embodiment.

specific Embodiment approach 3

[0019] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the metal ion described in step one is Fe 3+ , Mg 2+ 、Al 3+ , Ca 2+ , Zn 2+ 、Cd 2+ , Mn 2+ One or more of them are mixed in any ratio. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a method of increasing an oil yield and in-situ flocculation harvesting efficiency of microalgae growing in sewage. In order to solve the existing problems of a low oil content and a low recovery rate of the microalgae growing in the sewage, the method comprises the following steps: adding metal ions into the sewage, inoculating against a microalgae seed solution for fermentation after uniform mixing, after the microalgae fermentation is stable, discharging microalgae fermentation liquid, supplementing the equal amount of fresh mixed liquid A, adjusting a metal ion concentration to be the same as that in step I, continuing the fermentation after the uniform mixing, standing to allow flocs to settle to accomplish harvesting of the microalgae after the fermentation finishes, and extracting oil from the microalgae. As compared with the control group, the method increases the oil yield by 275% and the flocculation harvesting efficiency by 26.1%. A removal rate of pollutants in the sewage exceeds 96%. The method is applicable to the technical field of biochemical engineering.

Description

technical field [0001] The invention relates to a method for increasing the oil output of growing microalgae in sewage and the efficiency of in-situ flocculation harvesting. Background technique [0002] Microalgae has a high growth rate and oil content, and its production does not need to occupy arable land resources, so microalgae is considered to be a promising raw material for the production of renewable energy such as biodiesel. However, there are still many bottlenecks to be solved in the industrialization of microalgae bioenergy, including how to improve the oil production and harvesting efficiency of microalgae. At present, optimization of nitrogen source, phosphorus source, pH, light intensity, light cycle, temperature, etc. are often used to increase the oil production of microalgae, and more methods need to be discovered to promote the accumulation of oil by microalgae. In addition, microalgae are small in size and negatively charged on the surface, and are often...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463
Inventor 任宏宇孔凡英刘冰峰任南琪马军
Owner HARBIN INST OF TECH
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