A method and application of directly using non-detoxified acid to pretreat lignocellulose for hydrogen production
A lignocellulose and acid pretreatment technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reducing production costs, changing metabolic pathways, and good applicability
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Examples
Embodiment 1
[0068] Example 1: Hydrogen production capacity of different concentration substrates in synchronous saccharification
[0069] (1) Preparation of a seed fluid of thermophilic fluid oires: The thermophilic polytenee anaerobacterium MJ1 preserved -80 ° C is first passed through 10 ml of Willin bottle (with 4.5 mL seed medium) at 55 ° C, 150 rpm oscillating culture. 18h was activated, followed by amplification at 100 mL of serum bottle (45 mL seed medium) at 55 ° C, 150 rpm oscillated culture for 18 h, resulting in seed fluid. The seed medium is a xylose liquid medium containing 5 g / L xylose. The main components of the medium are: xylose 5g / L (carbon source), 1 g / L of ammonium chloride, 1 g / L of sodium chloride, hydrogen phosphate 1 g / L of potassium, 1 g / L of phosphate, 0.5 g / L, hexamine chloride 0.5 g / L, potassium chloride 0.2g / L, yeast powder 2g / L, protein 胨 2g / L, Trual element reservoir 1 ml / L, 1ml / L of vitamin reservoir, 0.01% blade Tianqing 1ml / L;
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Embodiment 2
[0077] Example 2: Hydrogen production capacity of different concentration substrates in conjunctive synchronous saccharification
[0078] (1) Preparation of seed fluid as in Example 1.
[0079] (2) Preparation of fermentation medium and sterilization as in Example 1.
[0080] (3) The first stage in the preheating synchronous aerobacterial prehydration synchronous saccharide fermentation mode is hydrogenated: the cellulase CTEC2 is injected into the medium with a syringe of 20U / g canesets, and after 50 ° C is enzymatically dissociated after 72 h. Further, the prepared seed liquid was injected into the fermentation medium after 10% (V / V) inoculation, and the fermentation temperature was 55 ° C, the shaker was 150 rpm, and the fermentation was terminated after fermentation 120h. Such as figure 2 As shown, the yield of hydrogen is increased as the substrate concentration increases, and the output is 20.71 mm (20g / L sugar canesets), 57.78 mm (40 g / L sugar cane), and 76.78 mm (6...
Embodiment 3
[0081] Example 3: Method for carrying hydrogen production in different fermentation modes
[0082] (1) Preparation of seed fluid as in Example 1.
[0083] (2) Preparation of fermentation medium and sterilization as in Example 1.
[0084] (3) The first stage of hydrogenation in synchronous saccharification fermentation mode in synchronous saccharification is in Example 1.
[0085] (4) EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc.
[0086] (5) Second stage fermentation hydrogen in different fermentation modes: After the end of the fermentation, synchronous glycained fermentation and prehydrolysis synchronous saccharification fermentation of the fermentation medium (100 g / L sugarcane slag) is adjusted to 7.0, The serum bottle was sealed with a glue and aluminum cover and repeatedly pumped with 0.01 MPa of nitrogen 3 times, transferred to the purity of the second stage, the fermentation temperature was 55 ° C, the shaker was 150 rpm, ferment 120h After the end of the fermentation. Such as im...
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Application Information
- IPC
- C12P3/00; C12R1/01
- CPC
- C12P3/00; C12P2201/00
- Inventors
- 朱明军; 胡彬彬