103 results about "ALPHA-ARBUTIN" patented technology

The invention discloses recombinant Bacillus subtilis for expressing L.mesenteroides sourced sucrose phosphorylase, belonging to the technical fields of genetic engineering and enzyme engineering. The recombinant Bacillus subtilis is prepared by the steps of acquiring a target gene of the L.mesenteroides sourced sucrose phosphorylase by virtue of an artificially synthesized SPase gene, a design primer and PCR, constructing a recombinant plasmid pBSMuL3-SP, and converting the recombinant plasmid pBSMuL3-SP into Bacillus subtilis (CCTCC M2016536), so as to obtain the recombinant Bacillus subtilis. The recombinant Bacillus subtilis is used as a strain for fermenting and producing SPase. The recombinant Bacillus subtilis has relatively good effect when being used for preparing alpha-arbutin. By recombining and expressing the sucrose phosphorylase by taking food-safety Bacillus subtilis as an expression host, the enzyme producing level is high, the separation and purification are convenient, the fermentation raw materials are wide in sources, and the production cost is relatively low.

The invention belongs to the technical field of cosmetics and particularly a biological skin brightening facial mask liquid and a preparation method thereof. The biological skin brightening facial mask liquid is prepared from the following components in percentage by mass: 0.1-2 percent of glabridin, 0.5-3 percent of microalgae skin brightening agent, 0.5-2.5 percent of citrus peel extract, 0.05-2percent of alpha-arbutin, 0.1-3 percent of radix paeoniae extract, 0.5-5 percent of yeast extract and the balance of matrix. The prepared facial mask liquid is capable of reducing melanin from multiple aspects and in multiple ways aiming to various parts in the process from generation to discharge of the melanin, has the remarkable effects of skin whitening and brightening, is capable of replenishing water, preserving moisture, resisting ageing and keeping skin healthy at the same time and does not have irritation.

The invention relates to a method for preparing alpha-arbutin which comprises steps of preparing slope seeds, selecting culture mediums, optimizing reaction conditions and extracting products and so on. The invention employs Xanthomonas campestris to ferment to produce the alpha-arbutin without use of organic solvents during the production and generation of harmful substances during the fermentation process. The invention has advantages of low production cost, simple process, and safe operation. And the alpha-arbutin yield produced by the method is high, the alpha-arbutin content in fermentation liquor is 8-11g / L, and the conversion rate of the hydroquinone can be 91%. The alpha-arbutin can obviously inhibit activities of tyrosinase, reduce tyrosinase deposition in skins, and bleach skins, remove freckles.

The invention belongs to the technical field of cosmetics, and discloses a skin care substrate with long acting moisturizing and whitening effects and preparation and application of skin care substrate. The skin care substrate is prepared from the following components in mass percent: 10%-20% of tremella polysaccharide, 5%-10% of glycosphingolipid, 10%-20% of saccharide isomerate, 5%-10% of nicotinamide, 3%-6% of alpha-arbutin, 3%-6% of tranexamic acid, 1%-3% of ADS-Oligonol and balance of water. The skin care substrate disclosed by the invention has obvious long acting moisturizing and skin whitening effects and can make the skin moisturized and bright white by persistent use.

A cosmetic preparation comprises an effective amount of Phyllanthus Embilca fruit extract and at least one oligopeptide such as Oligopeptide-4 and Oligopepticje-5 (pro-Elastin oligopeptide) The cosmetic preparation may further comprise Licorice extract, and Alpha-arbutin. It has surprisingly been found that this cosmetic preparation displays a beneficial synergistic effect for the combined treatment of fine line and wrinkles and / or skin brightening.

A process for catalytically synthesizing alpha-arbutoside from the free or immobilized cells of Xanthomonas campestris (CGMCC No.1243) includes such steps as preparing somatic cells, choosing reaction system, choosing immobilizing carrier, preparing immobilized cells, optimizing reaction condition, and extracting product. Said alpha-arbuto side can be used for beautifying skin.

The invention discloses a preparation method for alpha-arbutin. Starch matter and hydroquinone are adopted as raw materials, and biological enzymes are added for an enzyme method conversion reaction; after reaction liquid is subjected to enzyme removal, yeast is added for fermentation to remove glucose in the reaction liquid; after fermentation liquid is degermed, crude alpha-arbutin is obtained after primary crystallization, crude alpha-arbutin is dissolved for secondary or multi-time recrystallization, and alpha-arbutin with the purity of 99% or above can be obtained. Alpha-arbutin is produced through enzyme method conversion, and conversion efficiency is high. Yeast fermentation sugar removal is used for replacing a traditional column chromatography sugar removal process, acid or alkali or organic solvents used in the column chromatography process are avoided, and the use amount of pure water is greatly reduced. The method for purifying alpha-arbutin through the multi-time crystallization process is simple and convenient, and the product purity is controllable.

The invention belongs to the field of natural organic chemistry and relates to an extraction and separation method of alpha-arbutin from blueberry leaves. The method comprises the following steps: firstly extracting alpha-arbutin by using high voltage pulse electric field extraction equipment; then primarily purifying the alpha-arbutin by using membrane equipment and macroporous resin; then separating and purifying the alpha-arbutin by virtue of a simulated moving bed chromatographic separation technology to obtain a high purity alpha-arbutin product. The method provided by the invention has the advantages that 1, an extraction solvent can enter into the inner parts of plant cells by virtue of a high voltage electric field technology, so that the alpha-arbutin is efficiently extracted; 2, the alpha-arbutin is separated and purified by virtue of the simulated moving bed chromatographic separation technology, so that the alpha-arbutin obtained is high in purity. The prepared alpha-arbutin can be widely applied to the field of health foods, cosmetics and the like.

The invention discloses a genetically engineered bacterium for expressing sucrose phosphorylase and application thereof, and belongs to the fields of gene engineering and enzyme engineering. The genetically engineered bacterium has the advantages that a gene shown in SEQ ID NO.1 is subjected to heterologous expression in escherichia coli, recombinant escherichia coli is constructed, and is fermented to produce recombinant sucrose phosphorylase, and enzyme activity in fermented supernatant reaches 40U.mL<-1>; when the recombinant sucrose phosphorylase is used for catalyzing saccharose and hydroquinone to generate alpha-arbutin, a conversion rate can reach 91%; the industrial application potential is higher.

The invention discloses gene engineering bacteria producing alpha-arbutin and a construction method and application of the gene engineering bacteria. The invention provides recombinant bacteria which are obtained by a leading amylosucrase coding gene into target bacteria; amylosucrase is derived from Xanthomonas campestris. It is proved through experiments that the amylosucrase gene derived from Xanthomonas campestris CGMCC 1.3408 is led into colibacillus to construct the gene engineering bacteria, the gene engineering bacteria are used for producing alpha-arbutin, the conversion efficiency reaches 99%, the production intensity is 7.56 g / L / h, and the highest level as reported at home and abroad so far is achieved; meanwhile, maltose which is high in price is not used as donor in the production process, and enzyme purification is not needed. In this way, the production cost of alpha-arbutin is greatly reduced, and therefore the bacterial strain can have an extremely important position in alpha-arbutin production.

The invention relates to the field of cosmetics and provides an alpha-arbutin co-conveying nanometer composition. The alpha-arbutin co-conveying nanometer composition comprises alpha-arbutin, other skin-whitening active components and a nanometer medicine carrier; the alpha-arbutin accounts for 0.05 to 20.0 percent of the total mass of the co-conveying nanometer composition; other skin-whitening active components comprise at least one of a tyrosinase inhibitor outside the alpha-arbutin, a melanosomal migration inhibitor, an antioxidant, an anti-glycation active substance and an anti-inflammatory agent; the raw materials of the nanometer medicine carrier comprise an emulsifier and a co-emulsifier; and the alpha-arbutin co-conveying nanometer compositionl also comprises grease and water. Remarkable synergistic effect is achieved through compounding of different skin-whitening mechanisms and loading of the nanometer medicine carrier; the melanin content of skin is obviously reduced through multi-target-point effect; and the co-conveying nanometer composition can realize sustained release and controlled release of medicines, has large medicine carrying quantity, high stability, high water solubility, high skin permeability and excellent skin-whitening effect, and can be widely applied to cosmetics.

Polyelectrolyte based bioactive super-absorbent material ionotropically crosslinked / neutralized involving polyvalent carboxylic acids, citrate, Kojic Acid, Alpha Arbutin along with fish scale collagen cross linked with other polyelectrolyte biopolymers preferably selected from chitosan, alginate or their combinations is used in this invention. The advancement is also directed to process of extraction of collagen of high purity from fresh water fish scale by salt and alkaline washing, crushing followed by continuous dialysis thereby minimizing the chance of collagen degradation. Also disclosed are different forms of collagen-chitosan composite biomaterial using citrate as the neutralizing buffer in combination with antimicrobial agent, antioxidant, skin plumper and melanin reducer wherein the different forms of collagen chitosan particularly sheet, flakes, powder, gel, particles, fiber, film, spray etc. reveal efficient wound healing properties. The advancement is thus directed to find wide application in various dermal wound healing, tissue engineering, 3D cell culture, cell expansion and cell delivery vehicle, mimicking the in vivo situation in dynamic condition, cosmetics and different other health care applications.

The invention discloses a skin care matrix, a preparation method and an application of the skin care matrix as well as cosmetics and a preparation method of the cosmetics and relates to the technical field of cosmetics. The skin care matrix is mainly prepared from raw materials in percentage by mass as follows: 25%-30% of a citrus peel extract, 5%-10% of a Chinese radix rehmanniae extract, 2%-4% of nicotinamide, 1%-2% of alpha-arbutin, 1%-2% of sodium hyaluronate, 3%-5% of saccharide isomeride, 15%-20% of hydrolyzed prunus domestica and the balance of water. The problems that skin brightening cosmetics in the current market have relative single effects, poor moisturizing and skin moisture increasing effects and long effect cycle are solved. All raw materials of the matrix cooperate and can provide long-acting moisturizing and skin brightening effects. The skin care matrix is applied to skin care products, can make skin moist and vigorous, has the skin brightening and balancing effects and keeps the skin in a moist and bright state if used for a long term.

The invention relates to the composition of a cosmetic and a preparation method thereof, and in particular relates to an anti-wrinkle and whitening freeze-dried instant cosmetic and a preparation method thereof. In order to solve the problem of instability due to that raw materials in the existing whitening and anti-aging products are liable to hydrolyze, oxidize and the like, the invention provides an anti-wrinkle and whitening freeze-dried instant cosmetic and a preparation method thereof. The cosmetic comprises the following components: 2-16 parts by weight of excipient, 0.1-10 parts by weight of sodium hyaluronate, 0.0002-0.008 part by weight of EGF (epidermal growth factor), 0-5 parts by weight of VC (vitamin C), 0-0.5 part by weight of collagen, 0-3 parts by weight of alpha-arbutin, 0-0.5 part by weight of D-panthenol and 0-0.3 parts by weight of alpha-lipoic acid. The cosmetic is stable in characters and good in anti-wrinkle and whitening effects, and the preparation method is simple, and easy to operate.

The invention discloses a skin base liquid having a micro-ecological balance self-repairing function. The skin base liquid comprises, by weight, 0.05-0.1% of sodium hyaluronate, 0.05-0.2% of carbomer, 0.05-0.2% of triethanolamine, 0.05-0.1% of disodium ethylenediaminetetraacetate, 1.5-2.5% of lubrajel oil, 0.05-0.1% of methyl p-hydroxybenzoate, 2-3% of apple fruit cell culture extract, 0.5-1% of glycerol glucoside, 2-3% of ganoderma lucidum extract, 2-5% of palmitoyl tripeptide-5, 2-5% of alpha-arbutin, 0.5-1% of dendrobium officinale extract, 0.5-1% of caprylhydroxamic acid and 0.5-2% of azone. The skin base liquid can effectively repair cell damage, promote cell metabolism, make dark and uneven color skin bright and moist and make skin fine, soft and youthful.

The invention discloses a bioactive peptide skin activating, ageing preventing and whitening elite liquid and a preparation method thereof, which relates to the technical field of cosmetic. The elite liquid comprises the following ingredients: polyglutamic acid, betaine, hydroxyethylurea, elegant jessamine extract product, vanilla tahitensis fruit extract product, mandelic acid, acetyl hexapeptide-8, soluble proteoglycan, hydrolyzed collagen, tranexamic acid, sodium hyaluronate, alpha-arbutin, a yeast extract product, glycerin and butylene glycol. The preparation method is characterized in that one ingredient is added for fully stirring until a mixture is completely dissolved, and then the next ingredient is added. The raw materials have the advantages of no toxicity, no side effect, safety and reliability; the elite liquid is the multipurpose product which can be directly smeared or used for immersing a wet wipe, and the elite liquid is capable of improving skin metabolism, conditioning skin microcirculation, conditioning qi and blood of face, increasing moisture degree of skin, recovering elasticity, regenerating and activating skin, and has the efficacy of fining, whitening, preventing ageing and antioxidation.

The invention relates to a method for catalytic synthesis of alpha-arbutin by using dissociative or immobilized candida lipase. The method comprises the process steps of selection of a reaction system, preparation of immobilized lipase, optimization of reaction conditions, extraction of a product, etc. According to the invention, hydroquinone and sugar mixed according to a mol ratio of 1: 1 to 1: 5 are added into a reaction medium, and lipase is used as a catalyst for a reaction; the reaction lasts for 10 to 120 h at a temperature of 20 to 60 DEG C, and a conversion rate of alpha-arbutin is as high as more than 95%; and the usage amount of lipase is 0.01 to 5 times of the weight of sugar. Alpha-arbutin is synthesized under the catalysis of dissociative or immobilized lipase in the method for the first time, and the method has the advantages of mild reaction conditions, repeated usability of lipase, a short production period, a simple extraction process for the product, etc., thereby enabling production cost to be substantially reduced.

This invention is a topical tyrosinase inhibiting combination using alpha arbutin and bearberry extract, in synergism, for the purpose of skin lightening. The active admixture is a dermatological serum comprised of alpha arbutin, octyl stearate and polyolprepolymer-2, introduced onto the human skin in liposome form for ultra-deep penetration.

The present invention relates to a skin regenerating repair and anti-aging mask for electromagnetic radiation and fog-haze pollution, and a preparation method thereof, and belongs to the field of skin care products. According to the present invention, the mask cloth is formed by weaving charcoal fibers and plant wood pulp, and the mask solution essence comprises the following raw materials 8-20% of a ganoderma lucidum extract, 4-10% of a human placenta extract, 1-3% of acetyl hexapeptide-2, 1-3% of a silphium perfoliatum extract SOD, 1-3% of tocopherol, 1-3% of tranexamic acid, 3-5% of a snail mucilage extract, 0.05-2% of a hamamelis virginiana extract, 0.3% of epidermis cell growth factor (EGF), 0.05-3% of sodium hyaluronate, 0.3% of alpha-arbutin, 0.01% of EDTA tetrasodium, 0.2% of triethanolamine, 11% of butanediol, and the balance of deionized water. According to the present invention, the mask has effects of deep absorption and removal of all dirt on skin, oxygen free radical scavenging, resistance of harm of electromagnetic radiation and fog-haze pollution on skin, moist, silky, delicate, uniform and clean skin state achieving, and young state achieving.

The invention relates to the technical field of cosmetics, in particular to a slow-release whitening composite moisturizing face cream matrix as well as a preparation method and an application thereof. The face cream matrix is prepared from the following components in percentage by weight: 1-8% of alpha-arbutin, 6-11% of nicotinamide, 6-11% of licoflavone, 1-10% of sodium hyaluronate, 1-10% of algal polysaccharides, 1-10% of quaternary ammonium salt-73, 1-5% of azelaic acid, 1-10% of lecithin, ceresine wax, lanolin alcohol, paraffin and water. The slow-release whitening composite moisturizingface cream can improve the skin condition obviously if being used persistently.

The invention discloses a method for the synthesis of alpha-arbutin. The method comprises: heating a substrate, boron trifluoride etherate and a certain amount of a reaction medium to 30-80 DEG C to react for more than 48h, neutralizing, washing, drying, and concentrating to obtain an alpha-type arbutin intermediate with a relatively high purity, and thus obtaining the alpha-arbutin after hydrolysis and recrystallization. The method of the invention can improve the proportion of alpha / beta-glucoside, or convert a beta-type arbutin intermediate into an alpha- type arbutin intermediate, alpha-type isomer in the obtained intermediate product is high in purity (>=92%), easy to purify, and the obtained alpha-arbutin product has a purity of more than 98%.

The invention discloses a skin-whitening and freckle-removing skin care product and a preparation method thereof. In term of total weight of the skin care product, the skin care product is prepared from skin-whitening and freckle-removing functional components as follows: 0.2%-0.5% of alpha-arbutin, 2%-6% of ascorbic acid polypeptide, 2%-4% of nicotinamide and 1%-1.5% of phenylethyl resorcinol. Bymeans of compounding use of the four whitening agents and combination with other functional components, the skin care product can remarkably improve skin conditions from five aspects of brightening skin color, removing color spots, removing UV spots, eliminating chloasma and eliminating red zone and effectively removes freckles and whitens skin.

The invention discloses a composition capable of whitening and fading spots. The composition is prepared from the following raw materials in percentage by weight: 1 to 10 percent of humectant, 1 to 10percent of allergy-resisting allantoin, 1 to 10 percent of nicotinamide, 1 to 10 percent of a tightening and wrinkle-resisting agent, 1 to 10 percent of gold nanorod, 3 to 15 percent of VC-ethylether, 0.5 to 5.0 percent of alpha-arbutin, 14 to 55 percent of a solvent filling reinforcer and 3.0 to 15 percent of diglucosyl gallic acid, wherein the total amount of all the materials is 100 percent. The composition capable of whitening and removing the spots, disclosed by the invention, can be directly combined with tyrosinase, so that oxidation of the tyrosinase and dopa can be blocked, the decomposition and the excretion of melanin are accelerated, the activity of the tyrosinase in the skin is effectively inhibited and the formation of the melanin is blocked and further the efficacy of fading the spots and whitening is realized; the composition capable of whitening and fading the spots can be applied to downstream cosmetics; in addition, the composition has good spots-fading and whitening effects and low toxicity and meets the requirement of cosmetic regulations.

The invention discloses a whitening anti-aging skin softening lotion and a preparation method thereof. Active ingredients in the skin softening lotion include, by weight, 0.1-1 part of hirudin, 0.2-0.4 part of astaxanthin, 2-3 parts of tranexamic acid, 0.01-0.05 part of melatonin, 4-6 parts of glutathione, 1-3 parts of alpha-arbutin, 0.6-1 part of resveratrol, 0.1-0.2 part of gallogen, 0.1-0.5 part of selenium cysteine, 0.02-0.4 part of GSH-Px, 0.2-0.8 part of SOD, 0.6-2 parts of vitamin C ethylether, 0.4-0.6 part of nicotinamide, 2-4 parts of carnosine and 1-2 parts of green plum extract. Theskin softening lotion inhibits activity of tysocinase in melanoblast and cut off synthesis path of melanin, then further inhibits dopamine peroxidation, reduces melanin and delay skin cell aging; selenium cysteine and GSH-Px are utilized to collaboratively catalyze to participate in peroxidation reaction, so that whitening and anti-aging effects are further enhanced, and the skin softening lotionhas good moisturizing performance.

The invention relates to a skin whitening and brightening composition. The skin whitening and brightening composition is made of 2%-5% of fructus evodiae extract, 1%-4% of niacinamide, 1%-4% of mulberry bark extract, 0.2%-2% of alpha-arbutin, 0.2%-2% of hydroxyethylpiperazine ethane sulfonic acid, 0.1%-1% of radix glycyrrhizae extract, 2%-6% of butanediol, 1%-5% of glycerin, 0.1%-1% of preservatives, 0-0.1% of essence and the balance water by means of mixing. The skin whitening and brightening composition has the advantages that raw materials for the skin whitening and brightening compositionare mild, the skin whitening and brightening composition is good in stability, active components of the skin whitening and brightening composition mostly come from natural plants or mostly are biological preparations, accordingly, the skin whitening and brightening composition is mild for skin, and irritation and potential risks of formulas can be reduced; synergistic effects can be realized by various components of the skin whitening and brightening composition, accordingly, integral effects can be greatly improved, and effects of reducing melanin, whitening the skin and brightening skin colors can be realized.

The invention discloses a whitening and anti-freckle composition. The composition is prepared from, by weight, 2-10 parts of ascorbic acid, 0.4-2 parts of kojic acid, 0.1-2 parts of tranexamic acid, 0.1-2 parts of alpha-arbutin, 0.1-1 part of hydroxyethylpiperazine ethane sulfonic acid and a 3-15 parts of tartaric acid and / or a precursor. The whitening and anti-freckle composition reduces generation of melanin by inhibiting the activity of tyrosinase, promotes skin cell metabolism through acidized and softened cutin of the tartaric acid and / or the precursor and reducing damage of ultraviolet rays to the skin while improving the effective components by taking the hydroxyethylpiperazine ethane sulfonic acid as a biological buffer; by means of the whitening and anti-freckle composition, formation of the melanin is effectively reduced through effects in multiple aspects, and the skin is transparent. The invention further provides a skin moisturizer containing the whitening and anti-frecklecomposition and a preparation method of the skin moisturizer.

The invention discloses a smearing type hydro lifting needle without an additive and a preparation method of the smearing type hydro lifting needle. The smearing type hydro lifting needle comprises the following main ingredients: thermal spring water, a bio-saccharide gum-1, alpha-arbutin, glycoprotein, glutamic acid, valine, threonine, butanediol, 3-o-ethyl ascorbic acid, sodium hyaluronate, polypropylene glycol-26, p-hydroxyacetophenone, a saussurea involucrata extract, oligopeptide-1, a peony root extract, deer bone collagen, 1,2-hexanediol, a sambucus nigra fruit extract, fullerene, a kiwifruit extract, an xanthan gum and EDTA (Ethylene Diamine Tetraacetic Acid) disodium, the various components are mutually synergic through proper matching of the ingredients, and with the scientific preparation method, the smearing type hydro lifting needle having multiple effects of resisting oxidation, whitening, moisturizing, causing low allergy and repairing is prepared.

The invention belongs to the technical field of cosmetics and particularly relates to a skin whitening composition, a set comprising the kin whitening composition, and a preparation method thereof. The invention provides the skin whitening composition, the set comprising the kin whitening composition, and the preparation method thereof, by using the collaboration of carnosine, nonapeptide-1, yeast essence, alpha-arbutin, dipotassium glycyrrhizinate, dimethyl sulfone and a plant natural preservative, the composition with a skin whitening effect is prepared, the skin whitening composition provided by the invention can promote a skin- whitening agent to enter into a corium layer, not only the skin whitening effect can be improved greatly, but also the formula is gentle and cannot cause sensibilization. In addition, the kin whitening composition can be prepared into a set of skin toner, essence and face cream, a superior skin whitening effect can be played after combined use.

The invention provides a method for recycling sucrose phospholylase in an alpha-arbutin production process. Alpha-arbutin is synthesized by taking sucrose and hydroquinone as substrates under the catalysis of dissociative sucrose phospholylase rough liquid as a catalyst in the alpha-arbutin production process; after the reaction, an additive with a certain concentration is firstly added in a reaction liquid to improve the stability of the reaction liquid, then un-soluble floss materials and some large molecular proteins are filtered away by using a ceramic film, next, the dissociative sucrose phospholylase is recovered by using an ultra-filter film with a relatively small aperture, and finally the catalysis of the next batch is carried out by using the recovered sucrose phospholylase; and the purpose of recycling sucrose phospholylase is achieved by repeating the operations. The invention provides the method for recycling the sucrose phospholylase for the first time; in addition, the method has great significance in reducing film pollution, prolonging the service life of the film, reducing the production cost, and the like, so that the dissociative sucrose phospholylase has an excellent application prospect in the enzyme catalysis field.

The invention discloses a stock solution and a preparation process thereof. The stock solution comprises a phase A (deionized water, bio-saccharide gum-1, a first skin conditioner, a second skin conditioner, and the like), a phase B (butanediol, and the like), a phase C (a third skin conditioner, a fourth skin conditioner, a whitening and freckle removing agent, and the like); the first skin conditioner comprises at least one of macromolecular sodium hyaluronate, low molecular sodium hyaluronate and oligomeric sodium hyaluronate; the third skin conditioner comprises water for the third skin conditioner, glycoprotein, glutamic acid, valine, threonine, phenoxyethanol, ethylhexyl glycerol and sodium metabisulfite; the fourth skin conditioner comprises water for the fourth skin conditioner, adunaliella salina extract, phenoxyethanol, sodium benzoate and potassium sorbate; the whitening and freckle removing agent comprises at least one of resveratrol, alpha-arbutin and 3-o-ethyl ascorbic acid. The stock solution in the invention has the advantages of easy absorption, moisture retention, good anti-aging effect and prevention of melanin formation.