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Preparation method for alpha-arbutin

A technology for arbutin and substance, which is applied in the field of new preparation technology of α-arbutin, can solve the problems of high price of α-arbutin, difficulty in realizing large-scale production and unstable product quality, etc. The effect of reducing the consumption of pure water, controllable purity and high production efficiency

Inactive Publication Date: 2016-03-16
HUBEI ARTEC CARBOHYDRATE CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, α-arbutin used in whitening cosmetics in the world is mainly provided by Glico Factory in Jiangqi, Japan. Jiangqi uses enzymatic conversion to produce α-arbutin, and the post-extraction process mainly uses organic solvent extraction to separate unreacted Hydroquinone, chromatographic column adsorbs α-arbutin and sugar, and uses different concentrations of methanol to elute and separate sugar and glycoside (EP1260211A1). Due to the complicated post-extraction process and high cost, the international sales of α-arbutin The price has been high
There are also some companies in China that produce α-arbutin, mainly using Xanthomonas cell catalysis (CN200510080364), and then extracting using macroporous adsorption resin for separation and purification (CN200410090980.5), also using organic solvents for elution, and a large amount of acid-base Regenerated resin, high production cost, unstable product quality, difficult to achieve large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 20g of maltodextrin is heated and dissolved in 100ml of distilled water (i.e. the mass volume percentage of maltodextrin and distilled water is 20%, maltodextrin mass g / distilled water volume ml), add 1g of hydroquinone (i.e. hydroquinone and distilled water The mass volume percentage is 1%, hydroquinone mass g / distilled water volume ml), and the pH value of the entire reaction solution is adjusted to 5.

[0028] According to the standard of adding 100U per g of maltodextrin, add α-glycosyltransferase to the system and react at 40°C for 48 hours; 1000U standard was added with glucoamylase, and hydrolyzed at 55°C for 5h. The reaction solution after hydrolysis is a mixture of hydroquinone, glucose, α-arbutin and enzyme; hydroquinone, α-arbutin and glucose in the reaction solution are detected and analyzed by HPLC as follows:

[0029] (1) The detection condition is Connaught (CoMetro) high-pressure gel chromatography system, chromatographic column: Agilent ZORBAXSB-C18 (4...

Embodiment 2

[0033] 1g starch is heated and dissolved in 100ml distilled water (i.e. the mass volume percentage of starch and distilled water is 1%, starch mass g / distilled water volume ml), add 10g hydroquinone (i.e. the mass volume percentage of hydroquinone and distilled water is 10%) %, hydroquinone mass g / distilled water volume ml), adjust the pH value of the entire reaction solution to be 6.

[0034] According to the standard of adding 100U per g of starch, add α-amylase to the system, and react at 60°C for 24 hours; after the reaction is completed, add glucoamylase (that is, glucoamylase), and add glucoamylase according to the standard of 1000U per g of starch , Hydrolyzed at 55°C for 12h. The reaction solution after hydrolysis is a mixture of hydroquinone, glucose, α-arbutin and enzyme; hydroquinone, α-arbutin and glucose in the reaction solution are detected and analyzed by HPLC as follows:

[0035] (1) The detection condition is Connaught (CoMetro) high-pressure gel chromatograp...

Embodiment 3

[0039] 10g cyclodextrin is heated and dissolved in 100ml distilled water (i.e. the mass volume percentage of cyclodextrin and distilled water is 10%, starch mass g / distilled water volume ml), add 1g hydroquinone (i.e. the mass volume of hydroquinone and distilled water The volume percentage is 1%, hydroquinone mass g / distilled water volume ml), and the pH value of the entire reaction solution is adjusted to 5.5.

[0040] According to the standard of adding 100U per g cyclodextrin, add α-cyclodextrin glucosyltransferase (purchased from Angel Company) to the system, and react at 55°C for 36h; Enzyme), according to the standard of 1000U per g cyclodextrin, add glucoamylase, and hydrolyze at 55°C for 10h. The reaction solution after hydrolysis is a mixture of hydroquinone, glucose, α-arbutin and enzyme; hydroquinone, α-arbutin and glucose in the reaction solution are detected and analyzed by HPLC as follows:

[0041] (1) The detection condition is Connaught (CoMetro) high-pressur...

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Abstract

The invention discloses a preparation method for alpha-arbutin. Starch matter and hydroquinone are adopted as raw materials, and biological enzymes are added for an enzyme method conversion reaction; after reaction liquid is subjected to enzyme removal, yeast is added for fermentation to remove glucose in the reaction liquid; after fermentation liquid is degermed, crude alpha-arbutin is obtained after primary crystallization, crude alpha-arbutin is dissolved for secondary or multi-time recrystallization, and alpha-arbutin with the purity of 99% or above can be obtained. Alpha-arbutin is produced through enzyme method conversion, and conversion efficiency is high. Yeast fermentation sugar removal is used for replacing a traditional column chromatography sugar removal process, acid or alkali or organic solvents used in the column chromatography process are avoided, and the use amount of pure water is greatly reduced. The method for purifying alpha-arbutin through the multi-time crystallization process is simple and convenient, and the product purity is controllable.

Description

technical field [0001] The invention belongs to the technical field of green and environment-friendly production technology, more specifically, relates to a new preparation technology of α-arbutin, and no organic solvent is used in the whole process flow. Background technique [0002] α-arbutin is the epimer of β-arbutin, its chemical name is 4-hydroxyphenyl-α-D glucopyranoside, and its oxygen glycosidic bond is in the same direction as β-arbutin in space The direction of α-arbutin is just the opposite, and the optical rotation of α-arbutin [α] 20 D is +176~184°, β-arbutin is -63~-67°. α-Arbutin has a very good inhibitory effect on melanin synthase - tyrosinase, and its whitening effect on human skin is more than 10 times that of β-Arbutin, and it will not inhibit the growth of human cells and is non-toxic side effect. Studies have found that α-arbutin has a better therapeutic effect on scars caused by ultraviolet burns, and has better anti-inflammatory, repairing and whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44
CPCC12P19/44
Inventor 李丽刘红勇梁晓军刘默飞
Owner HUBEI ARTEC CARBOHYDRATE CHEM
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