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Process for preparing alpha-arbutin through fermentation

A technology of arbutin and fermentation liquid, which is applied in the direction of fermentation, etc., can solve the problems of low yield of α-arbutin, high price of α-glucosidase, and high cost of α-arbutin, so as to improve the safety of production operations , high output, and the effect of reducing production costs

Active Publication Date: 2005-07-06
CHENGZHI LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, domestic literature on the biological synthesis of α-arbutin has not yet been reported; the preparation method of α-arbutin abroad is mainly through the use of α-glucosidase as a catalyst to catalyze the synthesis of hydroquinone and glucose into α-arbutin Glycoside, because α-glucosidase is expensive, so the cost of α-arbutin produced is very high, and the yield of α-arbutin prepared by the above method is not high, which brings great difficulties to practical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Bacteria slant culture

[0043] Inoculate the slant culture medium of Xanthomonas campestris CGMCC NO.1243 strain into the test tube to make the slant strain;

[0044] Wherein the bacterial strain slant culture medium is made up of following components by weight:

[0045] 55 parts of sucrose, 15 parts of peptone, 3 parts of yeast powder, 0.5 parts of magnesium sulfate, 2.0 parts of dipotassium hydrogen phosphate, 2.0 parts of sodium chloride, 15 parts of agar and 1000 parts of water

[0046] The technological conditions for preparing slant strains are pH value 7.0, temperature 30° C., and incubation time 36 hours.

[0047] (2) Inoculation fermentation

[0048] Expand the cultivation of the slant strain as the seed solution;

[0049] Put the seed liquid into the fermenter according to the inoculation amount of 10% to ferment;

[0050] Wherein the seed liquid and the fermented liquid consist of the following components in parts by weight:

[0051] 55 parts of suc...

Embodiment 2

[0062](1) Bacteria slant culture

[0063] Inoculate the slant culture medium of Xanthomonas campestris CGMCC NO.1243 strain into the test tube to make the slant strain;

[0064] Wherein the slant culture medium is made up of following components by weight:

[0065] 45 parts of sucrose, 10 parts of peptone, 2.5 parts of yeast powder, 0.5 parts of magnesium sulfate, 2.0 parts of dipotassium hydrogen phosphate, 2.0 parts of sodium chloride, 15 parts of agar and 1000 parts of water

[0066] The technological conditions for preparing slant strains are pH value 7.5, temperature 30° C., and culture time 24 hours.

[0067] (2) Inoculation fermentation

[0068] Expand the cultivation of the slant strain as the seed solution;

[0069] Insert the seed liquid into the fermenter according to the inoculation amount of 15% to ferment;

[0070] Wherein the seed liquid and the fermented liquid consist of the following components in parts by weight:

[0071] 45 parts of sucrose, 10 parts o...

Embodiment 3

[0082] (1) Bacteria slant culture

[0083] Inoculate the slant culture medium of Xanthomonas campestris CGMCC NO.1243 strain into the test tube to make the slant strain;

[0084] Wherein the slant culture medium is made up of following components by weight:

[0085] 55 parts of sucrose, 10 parts of peptone, 2.0 parts of yeast powder, 0.5 parts of magnesium sulfate, 2.0 parts of dipotassium hydrogen phosphate, 2.0 parts of sodium chloride, 15 parts of agar and 1000 parts of water

[0086] The technological conditions for preparing slant strains are pH value 7.5, temperature 30° C., and cultivation time 36 hours.

[0087] (2) Inoculation fermentation

[0088] Expand the cultivation of the slant strain as the seed solution;

[0089] Insert the seed solution into the fermenter according to the inoculum amount of 12.5% ​​to ferment;

[0090] Wherein the seed liquid and the fermented liquid consist of the following components in parts by weight:

[0091] 55 parts of sucrose, 10...

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Abstract

The invention relates to a method for preparing alpha-arbutin which comprises steps of preparing slope seeds, selecting culture mediums, optimizing reaction conditions and extracting products and so on. The invention employs Xanthomonas campestris to ferment to produce the alpha-arbutin without use of organic solvents during the production and generation of harmful substances during the fermentation process. The invention has advantages of low production cost, simple process, and safe operation. And the alpha-arbutin yield produced by the method is high, the alpha-arbutin content in fermentation liquor is 8-11g / L, and the conversion rate of the hydroquinone can be 91%. The alpha-arbutin can obviously inhibit activities of tyrosinase, reduce tyrosinase deposition in skins, and bleach skins, remove freckles.

Description

technical field [0001] The invention relates to a preparation method of α-arbutin. Background technique [0002] At present, domestic literature on the biological synthesis of α-arbutin has not yet been reported; the preparation method of α-arbutin abroad is mainly through the use of α-glucosidase as a catalyst to catalyze the synthesis of hydroquinone and glucose into α-arbutin Glycoside, because α-glucosidase is expensive, so the α-arbutin cost of production is very high, and adopt above-mentioned method to prepare α-arbutin productive rate is not high, has brought great difficulty to practical popularization and application. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of α-arbutin, which is obtained by screening and mutagenizing Xanthomonas campestris to obtain a strain of α-arbutin with high yield and resistance to p-arbutin. Xanthomonas campestris CGMCC NO.1243, a strain with strong phenolic ability, sele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00
Inventor 刘春巧
Owner CHENGZHI LIFE SCI CO LTD
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