Method for catalytic synthesis of alpha-arbutin by using lipase
A technology of arbutin and lipase, which is applied in the direction of microbial-based methods, biochemical equipment and methods, and bulk chemical production, can solve the limitations of large-scale industrial production of α-arbutin, numerous microbial fermentation process steps, α-Glucosidase is expensive and other problems, to achieve the effect of easy large-scale industrial production, low price, and easy separation
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Embodiment 1
[0049] (a) Synthesis of α-arbutin catalyzed by free lipase
[0050] Add 0.11g (1mmol) of hydroquinone and 0.18g (1mmol) of glucose into 10ml of n-hexane, and react with 0.02g of free Candida lipase as a catalyst at a temperature of 40°C and a shaking table speed of 150r / min. Under the conditions, the reaction is 24 hours, and the conversion rate reaches 60%;
[0051] (b) Recovery and multiple use of free lipase
[0052] After the reaction is over, the free lipase can be recovered by centrifuging the reaction solution at 6000r / min for 15min, and the recovered lipase is put into the 10ml n-hexane reaction system containing 0.11g (1mmol) hydroquinone and 0.18g (1mmol) glucose again Continue the reaction in the middle; repeat the cycle so that the free lipase can catalyze the synthesis of α-arbutin for many times. Under this condition, the free lipase is converted for 5 cycles, and the conversion rates of α-arbutin are respectively: 60.0%, 59.0% %, 57.5%, 54.2%, 51.6%, so after ...
Embodiment 2
[0059] (a) Synthesis of α-arbutin catalyzed by free lipase
[0060] 0.11g (1mmol) hydroquinone and 0.36g (2mmol) glucose are added to 10ml of 10% tert-butanol containing dimethyl sulfoxide by volume, and the reaction takes 0.05g free Candida lipase as The catalyst was reacted for 72 hours at a temperature of 40°C and a shaker speed of 150r / min, and the conversion rate reached 86%;
[0061] (b) Recovery and multiple use of free lipase
[0062] After the reaction is over, the free lipase can be recovered by centrifuging the reaction solution at 6000r / min for 15min, and the recovered lipase is put into 10ml dimethyl-containing solution containing 0.11g (1mmol) hydroquinone and 0.36g (2mmol) glucose again. The sulfoxide volume fraction is 10% tert-butanol and continues to react; So repeated cycle, realizes that free lipase repeatedly catalyzes and synthesizes α-arbutin, under this condition, free lipase transforms 8 cycles, α-arbutin The conversion rates of fruit glycosides are:...
Embodiment 3
[0066] (a) Synthesis of α-arbutin catalyzed by free lipase
[0067] 0.11g (1mmol) hydroquinone and 0.54g (3mmol) glucose are added to 10ml of 10% tert-amyl alcohol containing dimethyl sulfoxide by volume, and the reaction takes 0.2g free Candida lipase as The catalyst is reacted for 72 hours at a temperature of 40°C and a shaker speed of 150r / min, and the conversion rate reaches 95%;
[0068] (b) Recovery and multiple use of free lipase
[0069] After the reaction, the free lipase can be reclaimed by centrifuging the reaction solution at 6000r / min for 15min, and the reclaimed lipase is put into the 10mL containing dimethylformamide containing 0.11g (1mmol) hydroquinone and 0.54g (3mmol) glucose again. The reaction is continued in tert-amyl alcohol whose volume fraction is 10% of sulfoxide; repeat the cycle so that the free lipase catalyzes the synthesis of α-arbutin multiple times. Under this condition, the free lipase converts 10 cycles, and α- The transformation rates of a...
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