33 results about "Arbutoside" patented technology

The invention discloses a method for synthesizing alpha-arbutin through biotransformation, and belongs to the technical field of biochemistry. A gene cloning technology is utilized, sucrose phosphorylase Spase in leuconostoc mesenteroides and sucrose phosphorylase gtfa in bifidobacterium adolescentis are screened and found through a Deep Molecules and DLKcat algorithm on a Uniprot database, novopro is utilized for codon optimization, then the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa are expressed on a plasmid vector PET-28a and are converted into an escherichia coli BL21 (DE3) strain, and the sucrose phosphorylase Spase and the sucrose phosphorylase gtfa in the bifidobacterium adolescentis are obtained. An engineering strain for heterologous expression of the gene is constructed by taking the gene as a chassis cell. According to the process, sucrose and hydroquinone are taken as co-substrates, and bioconversion is carried out under the action of sucrose phosphorylase from different sources to obtain alpha-arbutin.

The application discloses a preparation method of arbutin analogues with high transdermal absorption efficiency, and is characterized in that the method comprises the following steps: under the action of a solvent and a catalyst, compound I and compound II are subjected to esterification to obtain compound III, wherein the compound III is the arbutin analogue. The arbutin (compound I) is used as a raw material, the structure of the arbutin is modified through esterification, the transdermal performance of the arbutin is improved, the bioavailability is increased, and finally the whitening effect is improved.

This invention discloses a composition with synergistic whitening activity and its application in whitening and brightening daily chemical personal care products. β-Arbutin has a price advantage, but its whitening activity needs improvement. This invention provides a composition consisting of β-arbutin and turmeric ketone diol. In this composition, β-arbutin and turmeric ketone diol exhibit synergistic whitening activity. Combining turmeric ketone diol with β-arbutin significantly enhances the whitening activity of β-arbutin. Therefore, this composition can be used to prepare whitening and brightening daily chemical personal care products, such as facial cleansers, face creams, hand creams, lotions, and face masks.

The application discloses a mycobacterium maltose transferase Co GlgE and an encoding gene and application thereof.The maltose transferase Co The amino acid sequence of the GlgE is shown as SEQ ID NO.1.The application discloses a maltose transferase Corallococcus derived from mycobacterium sp.EGB Co GlgE, which can disperse maltoligosaccharide with a polymerization degree (DP) X into products with a DP of X ± 2n (X ≥ 3). Co The GlgE has glycosylation modification effects on alpha-arbutin and beta-arbutin, the biological activity of the modified arbutin is significantly improved, and the application value of the arbutin as an additive of whitening skin care products is improved.

The invention discloses a quantum chemical screening method for extracting arbutin, and relates to the technical field of natural product extraction, and the quantum chemical screening method comprises the following steps: constructing an arbutin single-molecule model and a hydrogen bond donor and hydrogen bond receptor single-molecule model by using GaussView software, firstly performing conformation search through MOPAC and molplus programs to obtain a most stable molecular structure, and then extracting the arbutin from the molecular structure; carrying out geometric optimization and single-point energy calculation by utilizing Gaussian; the optimized hydrogen bond donor and hydrogen bond acceptor establish random clusters according to different molar ratios, the random clusters with target molecules are established, a cluster model with an optimal structure is selected for geometric optimization and single-point energy calculation, meanwhile, the binding energy between the eutectic solvent and arbutin is calculated, and IGMH analysis, ESP analysis and AIM analysis are carried out. The invention provides a universal method for green and efficient extraction of active ingredients of natural products.

The invention discloses a whitening and spot-fading composition based on a fermented jasmine flower extract. The whitening and spot-fading composition comprises the following components in parts by mass: 0.1-1 part of a nano emulsifier, 0.5-8 parts of the fermented jasmine flower extract, 0.5-1.5 parts of arbutin and 1-3 parts of tranexamic acid. The nano emulsifier is a mixture of hydrogenated lecithin and phytosterol, the mass fraction ratio of arbutin to tranexamic acid is 0.5: 1, and the fermented jasmine flower extract specifically comprises a jasmine flower extract, a lactobacillus fermentation extract, water, 1, 2-hexanediol and 1, 2-pentanediol. The average particle size of the emulsion is about 256.56 nm through a nano emulsification technology, and compared with a conventional system, the transdermal absorption efficiency of active ingredients such as the jasmine flower extract, arbutin and tranexamic acid can be greatly improved, so that the active ingredients can reach action targets more easily; the problem of non-uniform dispersion caused by component solubility difference can be solved, the active component analysis risk is remarkably reduced, the system stability is improved, and the whitening and spot fading effects are enhanced.

The invention relates to the technical field of cosmetic component detection and analysis, and discloses a method for rapidly recognizing arbutin in cosmetics based on surface enhanced Raman spectroscopy, AgNPs sol is prepared by an ascorbic acid oxidation-reduction method, and an SERS sensing interface is constructed through aspartic acid electro-polymerization modification; for different dosage forms of cosmetics, a to-be-detected solution is obtained through methanol extraction and centrifugal filtration purification, the to-be-detected solution and AgNPs sol are mixed and adsorbed, then a Raman spectrum is collected, pretreatment is conducted through combination of multivariate scatter correction and standard orthogonal transformation, characteristic wavelengths are screened through a genetic algorithm-combined interval partial least square method, and detection is achieved through combination of qualitative and quantitative models. Through cooperation of sensing interface specific modification and a data processing technology, the arbutin signal recognition capability is enhanced, matrix interference is eliminated, rapid and accurate detection of arbutin in cosmetics is realized, and the effects of high sensitivity, high specificity and simple and convenient operation are achieved.

The application discloses a preparation method and application of a nano self-assembly composition of arbutin-berberine, and comprises the following steps: dissolving berberine in methanol at 38-42 DEG C, adding an arbutin super-pure water solution at 38-42 DEG C, and stirring and keeping warm for 0.8-1.2 h to obtain a self-assembly solution; then, the self-assembly solution is subjected to vacuum rotary evaporation and freeze drying in sequence, and the nano self-assembly composition of arbutin-berberine is obtained. The traditional Chinese medicine nano self-assembly system based on arbutin and berberine is successfully constructed, the nanoparticles are spontaneously formed under mild conditions through intermolecular non-covalent interaction, the preparation process is simple and convenient, the conditions are mild, the damage of high temperature and other harsh conditions to active ingredients is effectively avoided, and the process adaptability is high.

A whitening nanocomposition, a preparation method therefor, and use thereof. The whitening nanocomposition comprises an active ingredient and a nanocarrier. The active ingredient is coated and / or adsorbed by the nanocarrier. The active ingredient comprises the following components: 1-15 parts by weight of α-arbutin, 1-15 parts by weight of nicotinamide, 1-10 parts by weight of 3-o-ethyl ascorbic acid, 0.1-1 part by weight of a Centalla asiatica extract, and 1-5 parts by weight of retinyl palmitate. The nanocarrier comprises the following components: 5-10 parts by weight of an emulsifier, 5-20 parts by weight of a polyol, 0.1-5 parts by weight of a phospholipid, and 40-60 parts by weight of water. The whitening nanocomposition has good skin permeability and retention performance and enables high-concentration enrichment, prolonged retention, and sustained release or controlled release within a target tissue, so as to be effectively taken in by target cells, thereby improving bioavailability and enhancing the whitening effect.

The invention relates to application of 6 '-O-caffeoyl arbutin in preparation of a medicine for treating dementia, and belongs to the technical field of biological medicine. The 6 '-O-caffeoyl arbutin disclosed by the invention has a remarkable dementia treatment value, the action mechanism accurately fits the core pathological process of dementia, a neuroprotective effect is played by relieving oxidative stress and inhibiting inflammatory reaction, oxidative stress related indexes such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) can be effectively adjusted, and the dementia treatment effect is improved. The release of lactic dehydrogenase (LDH) and malondialdehyde (MDA) and the level of damage-induced nitric oxide synthase (I NOS) are reduced, so that the NMDA-induced PC12 cell damage is protected.

The invention provides a recombinant collagen patch for treating facial dermatitis and eczema and fading pigmentation and a preparation method of the recombinant collagen patch. The application comprises a base material layer and a functional composition loaded on the base material layer, wherein the functional composition comprises recombinant human collagen III, an anti-inflammatory active component and a whitening active component. Wherein the anti-inflammatory active component is a compound of ganoderma sinensis spore powder zymolyte, dipotassium glycyrrhizinate and asiaticoside in a specific mass ratio, and the whitening active component is an alpha-arbutin-ferulic acid condensate and a compound of tranexamic acid and glabridin in a specific mass ratio. And the base material layer is alginate-sodium carboxymethyl cellulose composite hydrogel which is modified by gamma-ray irradiation. Through the synergistic effect of all the active ingredients and the optimized preparation process, the multiple effects of resisting inflammation, repairing a barrier and fading hyperpigmentation are achieved, and the product is good in biocompatibility and remarkable in effect.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is as shown in formula (I). The invention also discloses the application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ABTB free radical scavenging capacity of the 6-O-Caffeoylbutin is higher than that of ascorbic acid and beta-arbutin, and a material basis is provided for developing novel antioxidants.

This invention belongs to the field of biochemical technology, specifically disclosing a 6′- O A whole-cell biosynthesis method for caffeoyl arbutin and its analogues includes the following steps: using aromatic carboxylic acids and vinyl acetates as substrates, performing a transvinylation reaction under palladium catalyst and base conditions to prepare aromatic vinyl esters; using the prepared aromatic vinyl esters as acyl donors, ... β Arbutin, using acyl acceptors, undergoes an aromatic acylation reaction in an organic solvent with whole cells of Aspergillus oryzae as a catalyst to yield the 6′- O Caffeoyl arbutin or its analogues. This invention develops a two-step whole-cell catalytic method for the efficient and sustainable synthesis of CA and its analogues. The synthesized compounds exhibit potent inhibitory activity against mushroom tyrosinase. This invention pioneers a novel green biosynthetic pathway for tyrosinase inhibitors, demonstrating significant application potential in the food preservation and cosmetics industries.

The invention discloses a whitening composition based on arbutin and nicotinamide and a preparation method thereof, and belongs to the technical field of cosmetics, the preparation method comprises the following steps: dispersing sodium alginate in hot water under high-temperature shearing, and hydrating to obtain a glue solution A; dissolving nicotinamide, alpha-arbutin, inositol and caprylyl glycol in dipropylene glycol to obtain a solution B; performing dry mixing on beta-1, 3-glucan and sodium polyglutamate, and performing high-speed shearing in hot water to obtain slurry C; cooling the slurry C, mixing with the solution B, and adding the glue solution A; and finally, standing and aging the system at 4 DEG C, spraying a trace calcium chloride ethanol solution for several times to induce the formation of a gel network, and adjusting the pH value. The technical problems that alpha-arbutin is easy to hydrolyze and inactivate and nicotinamide is high in irritation are successfully solved through a unique low-temperature ionic cross-linking gel process, the obtained product is excellent in stability, has a slow-release synergistic effect, can achieve a mild and efficient whitening effect without adding a chemical penetration enhancer, and can be applied to skin care products such as essence and ampoules.

The invention discloses an arbutin analogue, a preparation method thereof and application of the arbutin analogue in preparation of a medicine for treating chronic colitis, and belongs to the technical field of medicines. The arbutin analogue is structurally characterized in that a galactosyl group is connected with a polysubstituted benzene ring through a flexible connecting chain-(CH) n-O-to form a novel structure which is remarkably different from a known compound arbutin. The arbutin analogue is prepared through chemical synthesis, and a pharmaceutical composition containing the arbutin analogue is provided. Pharmacological experiments prove that the representative compounds hydroquinone monogalactoside and 3, 4-dihydroxyphenol galactoside show disease treatment activity obviously superior to that of arbutin in a DSS-induced mouse chronic colitis model, the disease activity index can be effectively improved, and colon tissue damage can be relieved. Therefore, the compound provided by the invention can be used for preparing medicines for preventing and / or treating chronic colitis. Formula (1)

The invention discloses a natural antibacterial agent arbutin energized self-polishing antifouling nano composite coating and a preparation method thereof.The preparation method comprises the steps that firstly, aminopropyl terminated polydimethylsiloxane and hexamethylene diisocyanate are synthesized into a prepolymer NPDI, then the prepolymer NPDI reacts with hydroxyethyl acrylate, and an organic matrix NPDA is obtained; the preparation method comprises the following steps: adsorbing arbutin by ZIF-8 to prepare ZIF-8 / Arb powder, blending the ZIF-8 / Arb powder with SiO2, adding NPDA, adding a fluorosilicone coupling agent, carrying out a blending reaction, and carrying out spraying and room temperature drying to prepare the self-polishing antifouling nano composite coating. The base body adopted by the coating is excellent in mechanical property and outstanding in structural stability, the lap joint shear strength reaches 14.08 MPa, the coating has the hydrolysis and low surface energy characteristics, and surface self-polishing and attached organism nondestructive stripping can be achieved under water flow shearing; the nano SiO2 improves wear resistance and durability, the ZIF-8 / Arb releases arbutin and Zn < 2 + > in a targeted manner in a subacid environment, synergistically and efficiently inhibits bacteria, inhibits biofilm formation, realizes long-acting active antifouling, and is suitable for a harsh marine environment.

This invention relates to the field of plant exovesicles, specifically to a Bletilla striata exovesicle complex loaded with arbutin, its preparation method, and its application. The preparation method includes the following steps: preparing a supernatant of Bletilla striata exovesicles; preparing an aqueous solution of arbutin; mixing the exovesicle supernatant with the arbutin aqueous solution, performing a cyclic freeze-thaw treatment, mixing thoroughly, allowing it to stand at room temperature for 30-40 minutes, centrifuging, and obtaining the precipitate, which is the complex. The exovesicle complex and its preparation method provided by this invention can reduce the melanin content of A375 cells by inhibiting melanin production-related genes while exerting a weak inhibitory effect on cells. Moreover, the inhibitory effect of the drug-loaded exovesicles is stronger than that of arbutin monomer at the same concentration, providing a certain research basis for the development of low-dose, high-efficiency skin whitening products.

The invention provides an anti-aging composition containing glutathione microspheres and a preparation method of the anti-aging composition. The anti-aging composition is prepared from the following components in parts by mass: 0.5 to 4 parts of arbutin, 1 to 5 parts of glutathione microspheres, 0.05 to 3 parts of ergothioneine, 0.005 to 0.02 part of oligopeptide-4, 1 to 5 parts of grape fermentation extract, 0.1 to 2 parts of sodium hyaluronate and 0.01 to 0.05 part of resveratrol substances. According to the anti-aging composition containing the glutathione microspheres, the skin roughness is lower, the skin brightness improvement rate is higher, the high DPPH free radical scavenging rate is achieved, after the anti-aging composition containing the glutathione microspheres is used for a long time, the skin can be smoother and brighter, meanwhile, DPPH free radicals can be effectively removed, and the anti-aging effect is good. And the antioxidant effect is better.

This invention relates to the field of Vaccinium bracteatum tissue culture technology, specifically to a method and application for increasing the content of arbutin and 6′-O-caffeoyl arbutin in Vaccinium bracteatum tissue culture seedlings. The method includes: using 1 / 4 MS medium as the rooting medium for the Vaccinium bracteatum tissue culture seedlings, wherein the specific concentrations of CaCl2·2H2O are 165-220 mg / L; KNO3 is 0-237.5 mg / L or 712.5-950 mg / L; MgSO4·7H2O is 0-46.25 mg / L; CuSO4·5H2O is 0.0375-0.05 mg / L; and MnSO4·4H2O is 0-11.15 mg / L. This invention achieves the controlled increase of the content of active compounds CA and Arb in Vaccinium bracteatum tissue culture seedlings by changing the mineral element concentrations in the 1 / 4 MS medium. This is an effective way to obtain large quantities of high-yielding CA and Arb Vaccinium bracteatum materials.

The invention discloses application of arbutin and pharmaceutically acceptable salts thereof in preparation of drugs for treating spinal cord injury. The arbutin has a remarkable effect of promoting spinal cord nerve repair, can improve nerve conduction disorder and motor function loss caused by spinal cord injury, is beneficial to recovery of normal physiological functions of an injured spinal cord area, and is expected to be developed into a new medicine for treating spinal cord injury.

1. The name of the design product: packaging kit (pearl arbutin cream). 2. The use of the design product: for containing products, packaging products. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: kit 1 perspective view. 5. Other circumstances that need to be explained: kit 1 is the outer packaging box of the product, and kit 2 is the inner packaging tube of the product. When used, kit 2 is placed in kit 1 for sale together.

The present application relates to a rock white bifunctional glycosyltransferase BpUGT1 Gene and application in preparation of arbutin and gastrodin, belong to the field of biotechnology. The rock white bifunctional glycosyltransferase BpUGT1 The gene nucleotide sequence is as shown in SEQ ID NO.1, and the sequence full length is 1431bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 476 amino acid residues are encoded. The rock white bifunctional glycosyltransferase BpUGT1 The gene can be used as the biosynthesis regulation gene of arbutin and gastrodin, and is applied to the preparation of arbutin and gastrodin, and the application prospect is remarkable, and easy to popularize and apply.

The invention belongs to the technical field of hair care products, and particularly relates to a composite acid gel composition as well as a preparation method and application thereof. The composite acid gel composition disclosed by the invention is prepared from the following components in parts by mass: 1.5 to 2.5 parts of capryloyl salicylic acid, 3.5 to 5 parts of glycolic acid, 0.8 to 1 part of arbutin, 0.05 to 0.1 part of piroctone olamine salt, 8 to 12 parts of a lotus-bacillus subtilis fermentation product, 5 to 6 parts of an emulsifying agent and 70 to 80 parts of water, the arbutin is prepared from alpha-arbutin and beta-arbutin. After capryloyl salicylic acid, glycolic acid, arbutin and a lotus-bacillus subtilis fermentation product are compounded, the composition can go deep into hair follicle sebaceous gland, dredge blocked pores and improve scalp folliculitis; the traditional Chinese medicine composition can effectively treat hair follicle deep blockage, accelerate old and waste cutin shedding, not only can solve the root problem of hair follicle deep blockage, but also can realize surface cutin renewal, and a product prepared from the traditional Chinese medicine composition is green, safe, mild and non-irritant, and can efficiently relieve scalp inflammation and inhibit dandruff.

The application discloses a use, an inhibitor and a skin care product of arbutin and high mountain firebrush extract, and can solve the problem of how to inhibit the expression level of the protein content of gamma H2AX. The DNA damage inhibitor comprises alpha-arbutin and high mountain firebrush extract, and the mass ratio of the alpha-arbutin and the high mountain firebrush extract is 0.5-70:1.

The invention discloses an anti-allergic inflammation composition and a preparation method and application thereof, the active ingredients of the composition comprise sphingomyelin and beta-arbutin, and the concentration ratio of the sphingomyelin to the beta-arbutin is 1: 2-2: 1. The sphingomyelin and beta-arbutin composite formula has a huge application prospect in the development of anti-allergic inflammation products (such as respiratory mucosa protective agents, skin anti-inflammatory care products, allergy prevention dietary supplements and the like).

The invention relates to the technical field of gene engineering, in particular to a sucrose phosphorylase mutant and application thereof in synthesis of alpha-arbutin. The preparation method comprises the following steps: mutating histidine at the 152nd site of wild type sucrose phosphorylase from streptococcus sanguineus into threonine and mutating isoleucine at the 336th site of the wild type sucrose phosphorylase from streptococcus sanguineus into proline, so as to obtain a sucrose phosphorylase mutant; the catalytic activity and the stability of the sucrose phosphorylase are obviously improved. When alpha-arbutin is synthesized by taking sucrose and hydroquinone as substrates through detection, the yield is increased by more than 40% compared with that of a wild type, and the catalytic reaction time can be greatly shortened.

The invention discloses a preparation method of a stabilizer, arbutin and vinyl oleate react to obtain the stabilizer, the invention also discloses a preservative and a preparation method of the preservative, and the preservative comprises a solvent, 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT), 2-methyl-4-isothiazolin-3-one (MIT) and the stabilizer. The stabilizer is added into the preservative for use, so that the chemical stability and application suitability of CMIT / MIT in a non-aqueous system can still be ensured while ions such as Ca < 2 + > and Mg < 2 + > are prevented from being introduced.

The application provides a kind of recombinant corynebacterium glutamicum for producing beta- arbutin and its construction method and application, belong to genetic engineering technical field, the recombinant corynebacterium glutamicum for producing beta- arbutin, is inactivated polyketide synthase gene on the basis of corynebacterium glutamicum again expression beta- arbutin synthesis gene after being obtained.The application inactivates polyketide synthase gene in corynebacterium glutamicum, and successfully obtains the strain 13032- Δpks13 with enhanced biofilm formation ability, can effectively enhance the adsorption growth ability of corynebacterium glutamicum cell on the surface of various media, overexpresses beta- arbutin synthesis gene in 13032- Δpks13 strain as fermentation strain, to realize the application of high-efficiency production of beta- arbutin in adsorption immobilization mode.

This invention discloses an *E. coli* Rosetta strain for the biosynthesis of α-arbutin. Recombinant genes for FruA, CscK, Pgi, and sucrose phosphorylase SmsP are inserted into the genome of the *E. coli* Rosetta strain, anchoring the sucrose phosphorylase SmsP protein product to the surface of *E. coli* cells. The invention also discloses the corresponding transposase plasmids and CRISPR plasmids, as well as the application of the strain in the catalytic synthesis of α-arbutin. This invention utilizes *E. coli* Rosetta (DE3) as the chassis cell and uses CRISPR transposition technology to enhance the FruA, CscK, and Pgi genes in the fructose metabolic pathway, thereby enhancing the metabolic pathway of the reaction's accompanying product, fructose. Enzyme anchoring technology is used to anchor sucrose phosphorylase to the surface of *E. coli*, allowing the sucrose phosphorylase to grow on the *E. coli* surface and catalyze the conversion of sucrose and hydroquinone from the external environment into α-arbutin and fructose.

The invention belongs to the technical field of biological prevention and control, provides application of arbutin to improvement of resistance of wheat to airborne diseases, and particularly provides application of arbutin to prevention and control of wheat stripe rust, leaf rust and powdery mildew. The arbutin is applied to improving the resistance of wheat to stripe rust, brown leaf rust and powdery mildew. The arbutin enhances the accumulation of wheat callose and improves the resistance of wheat to airborne diseases. The arbutin improves the expression quantity of the resistance genes TaPR1, TaPR2 and TaPR5, and improves the resistance of wheat to airborne diseases. Results of the invention show that arbutin can significantly enhance the resistance of wheat to leaf rust, stripe rust and powdery mildew. Fluorescent real-time quantitative PCR (Polymerase Chain Reaction) shows that after exogenous spraying of arbutin, the expression quantity of disease course defense reaction genes in wheat is obviously increased, and surface arbutin is a potential metabolite for enhancing wheat immunity and can be used for preventing and treating wheat rust.