129 results about "Arbutoside" patented technology

A process for catalytically synthesizing alpha-arbutoside from the free or immobilized cells of Xanthomonas campestris (CGMCC No.1243) includes such steps as preparing somatic cells, choosing reaction system, choosing immobilizing carrier, preparing immobilized cells, optimizing reaction condition, and extracting product. Said alpha-arbuto side can be used for beautifying skin.

The invention discloses a genetically engineered bacterium for expressing sucrose phosphorylase and application thereof, and belongs to the fields of gene engineering and enzyme engineering. The genetically engineered bacterium has the advantages that a gene shown in SEQ ID NO.1 is subjected to heterologous expression in escherichia coli, recombinant escherichia coli is constructed, and is fermented to produce recombinant sucrose phosphorylase, and enzyme activity in fermented supernatant reaches 40U.mL<-1>; when the recombinant sucrose phosphorylase is used for catalyzing saccharose and hydroquinone to generate alpha-arbutin, a conversion rate can reach 91%; the industrial application potential is higher.

The invention relates to a method for catalytic synthesis of alpha-arbutin by using dissociative or immobilized candida lipase. The method comprises the process steps of selection of a reaction system, preparation of immobilized lipase, optimization of reaction conditions, extraction of a product, etc. According to the invention, hydroquinone and sugar mixed according to a mol ratio of 1: 1 to 1: 5 are added into a reaction medium, and lipase is used as a catalyst for a reaction; the reaction lasts for 10 to 120 h at a temperature of 20 to 60 DEG C, and a conversion rate of alpha-arbutin is as high as more than 95%; and the usage amount of lipase is 0.01 to 5 times of the weight of sugar. Alpha-arbutin is synthesized under the catalysis of dissociative or immobilized lipase in the method for the first time, and the method has the advantages of mild reaction conditions, repeated usability of lipase, a short production period, a simple extraction process for the product, etc., thereby enabling production cost to be substantially reduced.

The invention discloses a method for synthesizing alpha-arbutin by an enzymic method through catalysis. The method comprises the following steps of: 1, culturing a slant seed: taking a xanthomonas campestris CGMCC (China General Microbiological Culture Collection Center) NO.1243 strain, and inoculating the xanthomonas campestris CGMCC NO.1243 strain in a slant culture medium for culturing; 2, culturing a liquid strain: selecting a ring strain, inoculating the ring strain in a basic fermentation culture medium, and placing on a table concentrator for oscillating and culturing to obtain a seed culture solution; 3, adding the seed culture solution in the basic fermentation culture medium, oscillating and culturing for 24-48h, freezing, centrifugally collecting thalli, washing the thalli with a PBS (Phosphate Buffer Solution) for 2-3 times, ultrasonically crushing at a low temperature, centrifuging, and taking supernate, namely intracellular crude enzyme; and 4, adding cane sugar and hydroquinone in the intracellular crude enzyme for reacting, and separating and purifying a reaction product to obtain the alpha-arbutin. The method disclosed by the invention has the advantages of high production efficiency, high transformation rate of the hydroquinone, low cost, short process flow and the like.

The invention relates to a composition capable of whitening and brightening, a preparation method thereof and an application thereof in cosmetics and belongs to the technical field of the cosmetics. The composition capable of whitening and brightening comprises the following ingredients in percentage by weight: 25% to 30% of arbutin, 10% to 15% of vitamin C or derivative thereof, 15% to 20% of nicotinamide, 3% to 6% of serpin, 1% to 2% of ginsenoside and 30% to 35% of sunscreen agent. According to the composition provided by the invention, the arbutin, the vitamin C derivative and the nicotinamide generate a whitening effect, and meanwhile, the serpin is used for protecting protein components of skin cells, promoting the activation and regeneration of the cells and eliminating cytotoxicity resulting from hydroquinone generated after the arbutin is reduced, so that the actions of long-acting whitening and cell activating are achieved; the composition has the advantages of little skin irritation and long acting time when the composition is applied to the cosmetics such as facial masks, emulsions and cream.

The invention provides a method for separating hydroquinone from an alpha-arbutin fermentation solution. The method comprises the following steps: (1) tributyl phosphate is used as an extracting agent for extracting the alpha-arbutin fermentation solution, and further an extract phase and a raffinate phase are separated, wherein the volume ratio of the extracting agent to the alpha-arbutin fermentation solution is 1 to (0.5-2) and the extract phase contains the hydroquinone; (2) stripping the extract phase obtained in the step (1) by utilizing a strip liquor, so as to separate the hydroquinone from the extract phase conveniently, wherein the strip liquor is a basic solution or an acid solution and the volume ratio of the extract phase and the stripping liquor is 1 to (0.01-0.5). With adoption of the method, the hydroquinone in the alpha-arbutin fermentation solution can be extracted efficiently at low cost, the impurity removing rate of the hydroquinone can be improved, the highly purified alpha-arbutin can be obtained, the emission of wastewater is reduced, and the method has an important significance on improving the economical efficiency of alpha-arbutin prepared through a biologic method.

The invention relates to new application of 6'-O-caffeoyl-arbutin in the technical field of medicines for inhibiting melanin, particularly application of 6'-O-caffeoyl-arbutin or derivatives thereof, or a natural, synthetic or semisynthetic mixture containing the 6'-O-caffeoyl-arbutin, or a composition containing the 6'-O-caffeoyl-arbutin in preparing cosmetics for whitening skin and removing freckles or medicines for whitening skin and treating chromatodermatosis. The 6'-O-caffeoyl-arbutin can effectively inhibit and decompose melanin, thoroughly remove the melanin freckles, and have the effect of whitening skin and removing freckles; and the zebra fish acute toxicity test indicates that compared with arbutin, the 6'-O-caffeoyl-arbutin has the characteristics of lower toxicity, higher activity, higher efficiency and higher safety.

The application discloses small molecule peptide skin bottom layer repairing serum. The serum comprises the following components, 1-50 parts of pure water, 1-15 parts of glycerin, 1-15 parts of olivefruit extract, 0.1-15 parts of fullerene, 0.1-15 parts of tremella fuciformis extract, 0.1-15 parts of camellia oleifera leaf extract, 0.1-15 parts of squalane, 0. 1-15 parts of simmondsia chinensis seed oil, 0.1-15 parts of portulaca oleracea extract, 0.1-15 parts of C12-20 alkyl polyglycoside, 0.1-15 parts of C14-22 alcohols, 0.1-10 parts of centella root extract, 0.1-10 parts of arbutin, 0.1-10parts of nicotinamide, 0.1-10 parts of resveratrol, 0.1-10 parts of allantoin, 0.1-10 parts of sclerotium gum, 0.01-1 parts of oligopeptide, 0.01-1 parts of disodium ethylene diamine tetraacetic acid(EDTA), 0.01-1 parts of dipotassium glycyrrhizinate, 0.01-1 parts of ascorbic acid, 0.01-1 parts of caprylhydroxamic acid and 0.01-1 parts of glycerol caprylate. The serum is mild in nature, fine andsmooth but not sticky, and fresh and easy to absorb, and retains sufficient moisture of skin, regulates metabolism to make the skin healthier, inhibits melanin, removes free radicals, delays aging, diminishes inflammation and soothes the skin, moisturizes and whitens the skin, resists allergy and repairs the skin, removes acnes, repairs sensitive skin, and improves the moisture level of the skinto make the skin white and delicate, bright, moist and smooth, and thus, a pretty appearance is shown.

The invention discloses a method for producing alpha-arbutin by virtue of biological fermentation, wherein liquefied flour, bran, calcium carbonate, monopotassium phosphate and urea are used as a fermentation substrate, and hydroquinone is added in the fermentation substrate and fermented by aspergillus oryzae to prepare alpha-arbutin. The method specifically comprises the following steps: adding wheat flour in water and liquefying, and then adding bran, calcium carbonate, monopotassium phosphate, ethylenediamine tetraacetic acid disodium salt and urea; inoculating the activated aspergillus oryzae in the solution, culturing for a period of time under the condition of 38-40 DEG C, adding hydroquinone, uniformly mixing, keeping a fermentation temperature at 42-45 DEG C, and fermenting for a certain time, thereby obtaining a fermentation mixture; and finally separating and purifying the fermentation mixture to obtain alpha-arbutin. The method for producing alpha-arbutin by virtue of biological fermentation provided by the invention is simple in process, and easy for process control; moreover, the various materials and reagents are all common in the market, so that the production cost is low; the alpha-arbutin produced by the fermentation method provided by the invention is high in conversion rate and capable of being greatly subjected to industrial production.

The invention discloses a whitening and anti-aging composition, a cosmetic and a preparation method of the cosmetic, and relates to the technical field of cosmetics. The whitening and anti-aging composition consists of the following components: 0.05-3 parts of arbutin, 0.05-3 parts of ascorbic acid glucoside, 0.001-0.1 part of glutathione, 0.5-5 parts of nicotinamide, 0.1-2 parts of tranexamic acid, 0.0005-0.05 part of dipeptide diaminobutyryl benzyl amide diacetate, 0.00005-0.005 part of acetyl tetrapeptide-2, and 0.0001-0.005 part of oligopeptide-4. The arbutin, the ascorbic acid glucoside, the glutathione, the nicotinamide and the tranexamic acid are selected as the whitening components; the dipeptide diaminobutyryl benzyl amide diacetate, the acetyl tetrapeptide-2 and the oligopeptide-4 are selected as anti-aging components; the whitening components cover a whole melanin generation path, and full-path whitening is achieved; and the anti-aging components aim at various aging expressions, multi-aspect anti-aging is achieved, besides, the whitening components and the anti-aging components have a synergistic interaction effect, and the whitening and anti-aging effects of the composition can be remarkably improved through the combined action of the whitening components and the anti-aging components.

The invention relates to a method for producing an early-strength concrete water reducer from arbutin preparation waste liquid. The method comprises the following steps: firstly, carrying out oxidization pretreatment on arbutin waste liquid by virtue of hydrogen peroxide of which the weight is equal to 10%-50% of the arbutin waste liquid; and then, adding 1-5 parts of the arbutin waste liquid subjected to oxidization pretreatment into 100-200 parts of a polycarboxylate water reducer, uniformly mixing, and standing for 2-5 days, so as to obtain the early-strength concrete water reduce product. The method has the advantages that the reutilization of the arbutin waste liquid is realized, the process is simple, and the product is environmentally friendly and high in stability.

The invention discloses a preparation method of arbutin in Quezui tea and application of the arbutin in treatment of hyperuricemia. A monomeric compound arbutin is obtained by directional extraction and separation from the Quezui tea, and an arbutin acquiring way is provided. The invention also discloses application of the arbutin in treatment of hyperuricemia, namely, the new application of the arbutin in treatment of hyperuricemia. The invention provides a new drug guide, a preparation method and medical application for the arbutin in treatment of the hyperuricemia and related diseases.

The invention discloses a composition with whitening and spot fading effects, cosmetic and a preparation method thereof. The composition is mainly prepared from, by weight, 2-8 parts of nicotinamide,1-5 parts of glutathione, 1-5 parts of alpha-arbutin, 1-5 parts of phenethyl resorcinol, 1-5 parts of vitamin C polypeptide and 1-5 parts of Kalanchoe pinnata leaf extract. The composition and the cosmetic thereof can prevent melanin from gathering and transferring, and reduce and decolorize melanin; the activity of tyrosinase is inhibited, and oxygen free radicals are removed to change the melanin generation path; therefore, melanin generation is prevented, and whitening and spot fading effects are further achieved.

The invention belongs to the field of microbial engineering technology and relates to a novel fermentation method, a self-flocculating method, for preparing alpha-arbutin. According to the invention, xanthomonas campestris is used for fermentation production of alpha-arbutin. When a strain is inoculated into a shake flask for cultivation, millimeter-scale particles can be formed by self-flocculating within 4-8 h, and particle morphology growth is maintained during the follow-up scale-up culture. Immobilization of the strain in a fermentation broth is realized, and after-treatment process is reduced. Centrifugal separation of thalline is not required, and production cost of alpha-arbutin can be reduced. By the method, yield of alpha-arbutin is high, and the product can inhibit generation and deposition of melanin, remove color spot and effectively inhibit tyrosinase activity at low concentration.

The invention discloses a facial mask with the moisturizing function. The facial mask with the moisturizing function comprises, by weight, 6-14 parts of batryticated silkworms, 4-8 parts of tribulusterrestris, 12-16 parts of white perilla leaves, 8-24 parts of aloe, 12-20 parts of almonds, 7-21 parts of glycyrrhiza, 2-3.5 parts of humectant, 8-22 parts of eupatorium extracting solution, 8-12 parts of fig extracting solution, 5-10 parts of 1,3 butanediol, 0.3-0.5 part of polysorbate-20, 0.4-1 part of dipotassium glycyrrhizinate, 0.2-0.6 part of arbutin, 3-5 parts of guar gum, 0.2-0.6 part of hydrolyzed collagen liquid, 4-12 parts of lavender essential oil and 6-8 parts of citric acid. The facial mask with the moisturizing function has the advantages that good skin whitening and moisturizing functions are achieved, the skin can be kept moisturized for a long time, and the skin can be made shinier and more delicate.

The invention discloses a genetic engineering strain for synthesizing alpha-arbutin as well as a construction method and application of the genetic engineering strain, and belongs to the technical field of genetic engineering. The alpha-arbutin is produced through whole-cell catalysis of recombinant bacillus subtilis expressing sucrose phosphorylase (SmSP), and a new thought and method are provided for production of the alpha-arbutin. By optimizing an expression vector and promoter of the sucrose phosphorylase (SmSP), the expression quantity of the SmSP is increased, and the yield of the alpha-arbutin is increased to 41.0 g/L from 10.6 g/L of an original strain. Due to the fact that plasmid expression is not stable, an optimized gene expression cassette P43-gtfA is integrated into a B.subtilis WB600 genome, and a genetically engineered strain BSP43-SmSP is obtained. When the concentration of a substrate HQ is 50g/L, the sucrose concentration is 310.9 g/L, the thallus concentration OD600 is equal to 20, and a catalytic system catalyzes in a shake flask at 30 DEG C and 220 rpm for 20 hours, the yield of the strain alpha-arbutin is stable and is increased to 61.1 g/L compared with a contrast, and the molar conversion rate of the substrate namely hydroquinone is 49.4%.

The invention provides a hydro-optic injection for medical cosmetology and a preparation method thereof. The hydro-optic injection for medical cosmetology is prepared from the following components of70 to 80 parts of purified water, 15 to 25 parts of hyaluronic acid, 10 to 15 parts of collagen, 3 to 5 parts of biosaccharide gum-1, 5 to 6 parts of alpha-arbutin, 3 to 5 parts of glycoprotein, 3 to5 parts of glutamic acid, 4 to 6 parts of valine and 5 to 7 parts of threonine. According to the hydro-optic injection for medical cosmetology and the preparation method thereof, the formula of the water-light injection is comprehensive in component variety, the amino acid variety is complete, additional amino acids required by a human body can be met, the purity of each amino acid is extremely high, and each amino acid can be directly absorbed by the human body in a short time and cannot cause any damage to the human body; in addition, the hydro-optic injection has the effects of moisturizing, sunscreen and antibiosis besides the traditional effects, the effects of the injection are enriched, and the hydro-optic injection is safe and reliable to use, free of side effects, capable of beingused for a long time and capable of well meeting the use requirements of people.

The invention provides a cosmetic composition containing yeast fermentation product extract and a preparation method thereof, and relates to the technical field of cosmetic compositions. The cosmeticcomposition is prepared from following raw materials of, in parts by weight, 2 parts-5 parts of yeast fermentation product extract, 0.1 part-4 part of hyaluronic acid, 15 parts-25 parts of Vaseline, 2.5 parts-5 parts of carboxymethyl chitosan, 1 part-2 parts of arbutin, 2.5 parts-5 parts of neoagaro-oligosaccharide, 0.2 part-0.4 part of a composite preservative, 0.1 part-0.2 part of a preservativesynergist and 60 parts-80 parts of deionized water. The cosmetic composition has the advantages of scavenging free radicals and reducing the melanin synthesis rate, and is excellent in corrosion resistance, safe and non-toxic in preservative component, and convenient for long-term storage of the cosmetic composition.

A whitening toner is prepared from, by weight parts, 3-5 parts of ascorbic acid, 3-7 parts of waxberry leaf proanthocyanidin, 4-6 parts of chondroitin sulfate, 15-20 parts of aloe extracting solution, 3-6 parts of pyrrolidone carboxylic acid-triethanolamine, 0.5-1.3 parts of myrrh oil, 0.8-1.6 parts of babassu oleoyl amphoteric sodium acetate, 0.5-1.2 parts of hydrolyzed silk, 3-5 parts of sodium lactate, 6-9 parts of angelica root extraction liquid, 8-14 parts of arbutin, 5-11 parts of melothria heterophylla extract, 8-12 parts of phloretin, 2-4 parts of glycine and 5-10 parts of coconut oil. The whitening toner has the advantages of having effects of whitening, oil control, anti-wrinkle and aging resistance and being very excellent in moisturizing effect and free of side effects.

The invention belongs to the technical field of cosmetics, and provides a whitening and moisturizing mask and a preparation method thereof. The whitening and moisturizing mask comprises the followingcomponents in parts by weight: 2-5 parts of arbutin, 0.7-1.5 parts of tranexamic acid, 0.8-1.5 parts of a desert rose leaf cell extract, 1-3 parts of nicotinamide, 0.5-2 parts of a glycyrrhiza glabraroot extract, 5-20 parts of a ceramide nanoemulsion, 1.7-3.5 parts of ascorbic acid, 1-5 parts of a rhizoma menispermi extract, 0.9-2.5 parts of a luffa root extract, 0.5-2 parts of a manyflower gueldenstaedtid herb extract, 0.1-0.3 part of polyphosphate double salt, 0.9-2.5 parts of polyacrylic acid, 0.1-0.5 part of butanediol, 0.9-2.5 parts of glycerinum and 25-43 parts of water, wherein the ceramide nanoemulsion consists of the following components in parts by weight: 1-5 parts of ceramide, 2-10 parts of olive oil, 5-15 parts of glycerol, 3-8 parts of castor oil polyoxyethylene ether, 1.5-4parts of poloxamer and 35-62 parts of water. By adopting the technical scheme, the problem that in a mask in the prior art, ceramide is poor in dissolution and stability is solved.

The invention relates to a method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification. The preparation method of the environment-friendly alpha-arbutin comprises the following steps: bioenzyme-based alpha-arbutin synthesis: adding a 5-50 g/L alpha-arbutin enzyme into an enzyme reaction base solution, and reacting at 30-50 DEG C to obtain an alpha-arbutin primary product solution, wherein the enzyme reaction base solution for bioenzyme-based alpha-arbutin synthesis is composed of 5.0-15.0 wt% of disaccharide, 0.5-5.0 wt% of 1,4-hydroquinone, 0.02-0.08 wt% of sodium hydroxide, 0.1-2.0 wt% of aid and the balance of purified water; and purifying the alpha-arbutin primary product by water crystallization separation 3-7 times, thereby obtaining the environment-friendly alpha-arbutin. The method for preparing alpha-arbutin has the advantages of simple technique, high safety and low cost, and is suitable for mass production. The obtained alpha-arbutin product is environment-friendly, has the advantages of high purity, high stability and low sensitization, and can be used for various whitening products.

The invention provides a method for constructing a stably inherited genetic engineering strain for efficient biosynthesis of beta-arbutin and application of the genetic engineering strain. The method comprises the following steps: firstly, integrating genes for coding tyrosine lytic enzyme, coumaric acid coenzyme A ligase, beta-cinnamyl hydroxylase, beta-cinnamyl oxidase and beta-cinnamyl deacylase (phdC) in a host, and constructing a host strain capable of realizing high yield of p-hydroxybenzoic acid; secondly, genes for coding 4-hydroxybenzoic acid hydroxylase and glucosyltransferase are integrated in the host, and a strain capable of producing beta-arbutin is constructed. And finally, integrating a gene of a coding core pathway and a gene of a shikimic acid pathway onto a genome of the engineering bacterium, and knocking out a gene of a competitive pathway to construct a stably inherited beta-arbutin efficient biosynthesis gene engineering strain, so that the influence of unstable engineering bacterium production caused by application of plasmids is avoided; the method has an application prospect in industrial production of beta-arbutin.