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A method for rapidly evaluating the resistance of rice srbsdv varieties by detecting the virus proliferation rate in the coleoptile

A virus multiplication and coleoptile technology, applied in the field of agricultural science, can solve problems such as low accuracy and long identification period

Inactive Publication Date: 2019-09-24
创世纪复交生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The purpose of the present invention is to provide a short identification period and high accuracy for quickly evaluating the resistance of rice SRBSDV varieties by detecting the virus proliferation speed in the coleoptile, aiming at the shortcomings of the existing SRBSDV variety resistance indoor identification period, such as long and low accuracy. method

Method used

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  • A method for rapidly evaluating the resistance of rice srbsdv varieties by detecting the virus proliferation rate in the coleoptile
  • A method for rapidly evaluating the resistance of rice srbsdv varieties by detecting the virus proliferation rate in the coleoptile
  • A method for rapidly evaluating the resistance of rice srbsdv varieties by detecting the virus proliferation rate in the coleoptile

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Embodiment

[0050] In 2015, the identification method of this invention was used to conduct SRBSDV resistance identification on some rice varieties resources collected. Three rice varieties, Shunyou 109, IR24, and Fengliangyou 4 were set as the identification scales for high resistance, medium resistance, and susceptibility. Among them, Shunyou 109 had a disease resistance performance of 138 in the 2013-2014 indoor inoculation identification. Among the rice variety resource groups, it ranks 11, IR24 ranks 28, and Fengliangyou 4 ranks 69. Coleoptile collection time points were set to four time points on the 4th, 6th, 8th, and 10th days after the completion of inoculation, and 3 replicates were taken for each time point for each species.

[0051] Real-time fluorescent quantitative PCR obtains the RQ value of each species' periodic sampling time point, as shown in Table 1:

[0052]

[0053] Then draw the trend line of the SRBSDV proliferation index: take the average RQ value as the ordinate, and...

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Abstract

The invention discloses a method for rapidly evaluating rice SRBSDV variety resistance through detecting a viral multiplication speed in coleoptile. The method comprises the following steps: (1) selecting an identifying scaleplate; (2) performing hypoxia induction on the rice to be tested and identifying the coleoptile of the scaleplate variety; (3) inoculating the toxic sogatella furcifera on the coleoptile and performing dark culture; (4) performing fluorogenic quantitative PCR to obtain a relative transcript level (RQ value) of an SRBSDV virus S9 and a reference gene in each rice variety period time; (5) drawing an SRBSDV proliferation index trend line; and (6) evaluating the resistance level and the resistance grade of the rice variety to be tested on the Southern rice black-streaked dwarf virus according to the SRBSDV proliferation index trend line formula. By detecting the viral multiplication speed in the coleoptiles and rapidly evaluating the resistance of the rice SRBSDV germplasm resistance, the resistance level and the resistance grade of the rice variety on the SRBSDV from the molecular level in the rice germination stage are determined, the identification period is short and the reliability is high, and the method has big breeding application value.

Description

Technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method for quickly evaluating the resistance of rice SRBSDV varieties by detecting the virus proliferation speed in the coleoptile. Background technique [0002] Rice is a plant belonging to the genus Oryzae of the family Cyperaceae, Monocotyledon in the Angiosperm phylum. Rice is one of the three most important food crops. The sown area accounts for 1 / 5 of the sown area of ​​grain, and the annual output is about 480 million tons, accounting for 1 / 4 of the world’s total grain output. More than half of the world’s population Rice is the staple food and is also one of the most important cultivated crops in my country. Rice is native to tropical Asia and is one of the world's main food crops. The sown area of ​​rice in my country accounts for 1 / 4 of the country's grain crops, and the output accounts for more than half. The cultivation history has been 6000-7000 year...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6851
Inventor 不公告发明人
Owner 创世纪复交生物科技发展有限公司
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