Method for analyzing immunoregulation of deer fetus oligopeptides on macrophages

A technology of immune regulation and macrophages, which is applied in the field of biotechnology research and development and analysis, and can solve the problems that the research of immune regulation has not yet been carried out

Pending Publication Date: 2020-01-14
吉林省东北亚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, the research on the immune regulation of macrophage RAW264.7 by deer fetal oligopeptide has not yet been carried out

Method used

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  • Method for analyzing immunoregulation of deer fetus oligopeptides on macrophages
  • Method for analyzing immunoregulation of deer fetus oligopeptides on macrophages
  • Method for analyzing immunoregulation of deer fetus oligopeptides on macrophages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Take 40g of the deer viviparous product, crush it to a particle size of 60 mesh, take distilled water 10 times the mass of the deer viviparous product, add it to the pulverized deer viviparous product, and stir once at 4°C for 6 hour, the rotating speed is 3600r / min, after centrifugation for 10 minutes, take the supernatant. The yield was 0.139%.

Embodiment 2

[0078] Take 40g of the deer viviparous product, crush it to a particle size of 60 mesh, take distilled water 20 times the mass of the deer viviparous product, add it to the pulverized deer viviparous product, and stir twice at 4°C for 7 hours each time. hour, the rotating speed is 3600r / min, after centrifugation for 20 minutes, take the supernatant. The yield was 0.274%.

Embodiment 3

[0080] Take 40g of the deer viviparous product, crush it to a particle size of 60 mesh, take distilled water 25 times the mass of the deer viviparous product, add it to the pulverized deer viviparous product, and stir 3 times at 4°C, each time for 8 hours, the rotating speed is 3600r / min, after centrifugation for 30 minutes, take the supernatant. The yield was 0.436%.

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Abstract

The invention discloses a method for analyzing immunoregulation of deer fetus oligopeptides on macrophages, and belongs to the technical field of biotechnology research, development and analysis. After screening, the proliferation index of RAW264.7 cells is significantly higher than that of a blank group, and is closest to that of an LPS positive control group. The method analyzes the influence ofdifferent mass concentrations of deer fetus oligopeptides on the phagocytic function, the NO secretion amount and the release of cytokines such as IL-1 beta, IL-6 and TNF-alpha; and the obtained results show that, under the concentration of 25 mu g / mL, the NO has the maximum release amount and is closest to the LPS group. According to the method, cell cycle inspection is carried out simultaneously; data results prove that with the increase of the mass concentration of the deer fetus oligopeptides, the cell proportion in the G0 / G1 stage is in a trend of firstly rising and then falling, the cell proportion in the S stage and the G2 / M stage is in a trend of firstly falling and then rising, and the proportion of the cells in each stage is closest to that of the LPS group at the concentrationof 25 mu g / mL; the G0 / G1 stage is the synthesis period of RNA and protein, so that the expression quantity of related protein is increased, and the secretion amount of phagocytic action, NO and cytokines reaches the maximum value in the period.

Description

technical field [0001] The invention belongs to the technical field of research and development and analysis of biotechnology, and in particular relates to an analysis method for immune regulation of macrophages by deer fetal oligopeptides. Background technique [0002] The deer fetus is the placenta and fetus of the deer family animal sika deer (Cervus nippon Temminck) or red deer (Cervus elaphus Linnacus). Deer fetus is a traditional and precious tonic in my country, and it was known as one of the "Three Treasures of Gynecology" (deer fetus, black-bone chicken, and donkey-hide gelatin) in ancient times. Deer fetus has a long history as a traditional Chinese medicine to treat diseases, and its pharmacological effects were first recorded in "Compendium of Materia Medica". Deer fetus, regulates menstruation and nourishes skin, removes various poisons. Traditional Chinese medicine believes that deer fetus is mainly used for kidney deficiency, impotence, and insufficient nour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/68C07K1/34
CPCG01N33/543G01N33/68C07K1/34
Inventor 张辉张凯月吴楠杜延佳李志成李晶峰边学峰吕金朋兰梦高旭
Owner 吉林省东北亚生物科技有限公司
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