Culture medium for culturing olfactory ensheathing cells
A technology of olfactory ensheathing cells and culture medium, which can be used in animal cells, nervous system cells, vertebrate cells, etc., and can solve problems such as immune response, patient exposure, and impact.
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Embodiment 1
[0111] Embodiment 1, the cultivation of olfactory ensheathing cells in different mediums
[0112] 1. Culture of olfactory ensheathing cells
[0113] 1) Pretreatment
[0114] The isolated human olfactory ensheathing cells (Olfactory Ensheathing Cells, OECs, described in Lim F, Martín-Bermejo MJ, García-Escudero V, Gallego-Hernández MT, García-Gómez A, Rábano A, Díaz-Nido J, Avi la J, Moreno-Flores MT. Reversibly immortalized human olfactory ensheathing glia from an elderly donor maintain neuroregenerative capacity. Glia. 2010, 58(5): 546-58. Publicly available from Beijing Qingmei Lianchuang Stem Cell Technology Co., Ltd. .) Wash, digest with trypsin solution at 37.5°C for 3 minutes, collect the cells, centrifuge, discard the supernatant, and collect the cells.
[0115] PBS washing solution, Invitrogen, catalog number 10010031;
[0116] Tryptic Digest, Invitrogen, Cat. No. 25300054.
[0117] 2) Primary culture
[0118] The cells obtained in step 1) are fully blown into a s...
Embodiment 2
[0171] Embodiment 2, the cultivation of olfactory ensheathing cells in different mediums
[0172] 1. Culture of olfactory ensheathing cells
[0173] 1) pretreatment: same as embodiment 1;
[0174] 2) Primary culture: basically the same as in Example 1, the difference is that the cell culture medium 2 is used for culturing;
[0175] 3) Subculture: basically the same as in Example 1, the difference is that the cell culture solution 2 is used for culturing;
[0176] Cell culture solution 1 was prepared according to the following steps: DMEM medium (Invitrogen, 21041025), biotin, glutamine, basic fibroblast growth factor, epidermal growth factor, insulin, selenium, human albumin, tissue Component 1, component 2, component 3, component 4, component 5, component 6 and component 7 are mixed to obtain cell culture medium 1;
[0177] The component 1 is ferric chloride, chromium chloride, cupric chloride, manganese chloride, zinc chloride and sodium molybdate;
[0178] The component...
Embodiment 3
[0224] Embodiment 3, the cultivation of olfactory ensheathing cells in different mediums
[0225] 1. Culture of olfactory ensheathing cells
[0226] 1) pretreatment: same as embodiment 1;
[0227] 2) Primary culture: basically the same as in Example 1, the difference is that the cell culture solution 3 is used for culturing;
[0228] 3) Subculture: basically the same as in Example 1, the difference is that the cell culture solution 3 is used for culturing;
[0229] Cell culture medium 3 was prepared according to the following steps: DMEM medium (Invitrogen, 21041025), biotin, glutamine, basic fibroblast growth factor, epidermal growth factor, insulin, selenium, human albumin, tissue Component 1, component 2, component 3, component 4, component 5, component 6 and component 7 are mixed to obtain cell culture medium 1;
[0230] The component 1 is ferric chloride, chromium chloride, cupric chloride, manganese chloride, zinc chloride and sodium molybdate;
[0231] The component...
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