Tissue culturing method of high-quality papaya sprout

Abstract

A tissue culturing method for high-quality papaya sprout features that the adult lateral bud of the disease-resistant female papaya tree is chosen as explant, and the conditions for tissue culture and rooting culture are disclosed for higher effect.

Description

【Technical field】 [0001] The invention relates to a method for cultivating excellent seedlings from lateral bud cells of papaya hermaphrodite strains grown in the field. 【Background technique】 [0002] Papaya (Carica Papaya L.) is a perennial evergreen fruit tree crop widely distributed in tropical and subtropical regions of the world. Due to its fast growth, early results and high yield, it has become the most commonly cultivated fruit tree species in southern my country and tropical regions of the world. one. It has important food and industrial value, and is one of the important popularization projects for the development of tropical high-efficiency agriculture and one of the projects for the adjustment of tropical agricultural industry structure. The nutritional value of papaya is high, containing 12% soluble solids, and every 100g pulp contains vitamin A, B1, B2, C and G60-122mg. The milk of unripe green fruit contains a large amount of "papain", which can help digesti...

AI Technical Summary

Problems solved by technology

[0003] Problems in the rapid propagation of papaya: 1) Unfavorable factors in commercial production So far, the production and planting of papaya is still based on the traditional seed propagation method, and the plant sex of papaya is complex, with hermaphrodite plants, female plants and male plants Generally, hermaphroditic plants account for 65-70%, female plants account for 30%, and male plants account for 3-5%. Propagation with conventional seeds cannot guarantee the same plant sex, and only hermaphrodite plants have production value in actual production. The plants must be removed, so it can be seen that the production of seeds will cause a lot of waste; the cost of hybrid seed production is very high, the seeds are not resistant to storage, and are susceptible to damage by diseases and insect pests, especially the papaya ringspot virus disease that makes papaya from perennials. As an annual, these factors greatly limit the production and utilization of papaya

The rapid clonal propagation technology of papaya has been used in commercial production on a small scale. Although it has many advantages, some factors limit the application of this technology: (1) The tissue culture of papaya with good genotypes requires the use of well-grown mature plants in the field The buds of papaya, especially the side buds, are cultivated, and it is more difficult to cultivate the buds of mature papaya than the seedlings; (2) it cannot be subcultured indefinitely, and after cultivating 8-13 generations, the proliferation rate declines to some extent, and after 12-13 generations , no longer produce apical dominance; (3) field-resistant or virus-resistant papaya plants are difficult to find

2) Pollution The terminal buds or lateral buds of papaya picked directly from the field have a pollution rate of over 80% after disinfection, so most of the rapid propagation of papaya ends in failure

However, genetically modified papayas involve biological safety issues, which temporarily restrict the promotion and application of genetically modified papaya varieties with disease resistance. At the same time, considering the rights and interests of consumers, genetically modified foods are sold with labels. It is not generally accepted. Considering the continuity of the papaya planting industry, it is obviously an important remedial measure to use disease-resistant plants in the field as materials to produce high-quality seedlings in the near future; , Hermaphrodite papaya plants can produce high-quality, high-yield fruit, and the method of planting and producing seedlings commonly used at present obviously cannot achieve this purpose; the rapid propagation technology established with tissue culture technology can provide papaya The production of homogeneous high-quality seedlings is provided, and the use of rapid propagation technology to propagate excellent single plants with known traits is obviously the best way to achieve this goal. Although a lot of work has been done at home and abroad, it is not systematic, and there are still many technical problems to be solved urgently, such as the poor repeatability of the multiplication technology obtained by using a certain variety to another papaya variety, and many other problems such as induced rooting. The problems of low frequency and other links have not been completely broken through, which greatly limit the application of this technical system in the production of papaya seedlings