Method for inducing adventitious buds by adopting Lycoris radiate rachis as explant

A technology of Lycoris safflower and explants, which is applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of low proliferation rate, high pollution rate, and rapid propagation method of pollution rate cultivation has not yet been established, etc. Achieve high multiplication rate and transplanting survival rate

Active Publication Date: 2017-04-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have obtained the proliferation medium and rooting medium of other species of Amaryllis, most of which use bulbs as explants, and often have the problems of high pollution rate and relatively low proliferation rate
However, the rapid propagation method of Amaryllis plant tissue culture with high proliferation rate and low pollution rate has not yet been established.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Explant Screening

[0022] The bulb disc, inflorescence rachis and petal base of Lycoris safflower were used as explants to compare the contamination rate of different parts of the explants and the effect on the induction of adventitious buds of Lycoris safflower.

[0023] Disinfection methods are all using "washing detergent, shake for 15 minutes, rinse with running water for 30 minutes, disinfect with 75% ethanol for 30 seconds on a sterile operating table, and then use 0.2% Sterilized with mercuric chloride for 15 minutes, and finally washed 5 times with sterile water, 1 minute each time; ". Cultured in induction medium MS+6-BA 2mg / L + zeatin 0.3mg / L + GA 3 0.8mg / L. 100 explants were connected to each treatment, and the pollution rate, induction rate and induction multiple were counted after 30 days.

[0024] The test results are shown in Table 1. It can be seen from Table 1 that the best explant is the inflorescence rachis, which not only has a low contamination...

Embodiment 2

[0028] Induction medium screening

[0029] The inflorescence rachis was used as explants, and after disinfection, they were inoculated on induction medium containing different hormone ratios, and the effects of different medium on the induction of adventitious buds were compared. The development of explants was observed, and the induction rate and induction multiple of adventitious buds were counted after 30 days.

[0030] As can be seen from Table 2, in MS+6-BA 2mg / L + zeatin 0.3mg / L + GA 3 On the 0.8mg / L medium, the explants developed best, the induction rate was the highest, and the number of induced adventitious buds was the largest.

[0031] Table 2 The effect of induction medium on the induction of adventitious buds of Lycoris safflower

[0032] Medium formula Induction rate% Adventitious bud multiple MS+zeatin 2mg / L +NAA0.2 mg / L 88 2.4 MS+KT 2mg / L +NAA0.2 mg / L 74 1.1 MS+6-BA 2mg / L + Zeatin 0.3mg / L + GA 3 0.8mg / L

Embodiment 3

[0034] Disinfection method screening

[0035] Lycoris safflower is a perennial flower, and the explants taken are underground parts, so it is difficult to control the pollution rate. In order to reduce the contamination rate of explants, the effects of different combinations of disinfection methods on the contamination rate of explants were compared. Firstly, the pretreatment and disinfection methods were compared. The explants were treated with 84 disinfectant, Amway disinfectant, a mixture of penicillin and streptomycin for 20 minutes, and 10 minutes each of the three disinfectants were used for disinfection pretreatment. Then, transfer to the aseptic operating table, soak the explants in 75% alcohol for 30 seconds, and treat them with 0.2% mercury chloride for 10 minutes to disinfect the explants. Secondly, to compare the mercury disinfection time, use Amway washing liquid to shake and soak for 15 minutes, transfer to the sterile operating table, and soak in 75% alcohol fo...

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PUM

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Abstract

The invention provides a method for inducing adventitious buds by adopting Lycoris radiate rachis as explant in order to obtain regenerated plants. The method comprises the following steps: selection and disinfection of the explant, induction of the adventitious buds, subculture and propagation, root induction, and transplantation of tissue culture plants. The method allows the induction germination rate to reach 96%, 4.9 times or above adventitious buds to be proliferated from a per plant and the rooting rate to reach 94%, and can effectively solve the problem of in vitro rapid propagation of Lycoris radiate.

Description

technical field [0001] The present invention relates to Lycoris safflower ( Lycoris radiata (L'Her.) Herb.) tissue culture propagation method, which belongs to the technical field of seedling propagation of herbaceous ornamental plants. Background technique [0002] Safflower Lycoris ( Lycoris radiata (L'Her.) Herb.) is a kind of Amaryllidaceae Lycoris. Perennial herb. Underground stems are thick. Ligule-shaped, pumped from base after flowering. The scape is 30-60 cm high, the umbel is terminal, the flower is bright red, the flower tube is short, the perianth is narrowly oblanceolate, rolled outward, the stamens and styles protrude, the posture is beautiful, the flowering period is from July to September, Blooms in July and grows in summer. Sexually likes a damp environment, afraid of direct sunlight, and should grow in loose and fertile sandy loam. Native to China and Japan, it is widely cultivated all over the world. It is an excellent perennial herbaceous flower. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 不公告发明人
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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