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A standard comparison card for assisting in the interpretation of ki67 proliferation index

A KI67 and comparison card technology, applied in image enhancement, image data processing, instruments, etc., can solve the problems of unguaranteed reliability of results, lack of KI67 interpretation, etc., and achieve low cost, high accuracy and repeatability high effect

Active Publication Date: 2022-01-28
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is a lack of standardized methods for KI67 interpretation, resulting in large differences in KI67 interpretation results obtained by different observers or laboratories, and the reliability of the results cannot be guaranteed

Method used

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  • A standard comparison card for assisting in the interpretation of ki67 proliferation index
  • A standard comparison card for assisting in the interpretation of ki67 proliferation index
  • A standard comparison card for assisting in the interpretation of ki67 proliferation index

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 The making and using method of the standard comparison card of the present invention

[0027] Use the PRECICE 600 series automatic digital slide scanning system to scan breast cancer tumor sections stained with KI67 immunohistochemistry and convert them into whole slide image data. The area with the highest proportion of KI67 positive cells was selected as the "hot spot" area, and the proportion of KI67 positive cells in the hot spot area to the tumor cells in the hot spot area was judged by computer, which was the KI67 proliferation index, and the KI67 proliferation index was selected as 5%, 10%, and 15% respectively. %, 20%, 30%, 40%, 50%, 60%, 70%, 80% of the area, intercept the area of ​​the picture, and print it in color.

[0028] When in use, it is only necessary to compare the KI67 immunohistochemical staining section of the breast cancer to be tested with each standard comparison card to find the closest standard comparison card, and the KI67 prolif...

Embodiment 2

[0029] Embodiment 2 The making and using method of the standard comparison card of the present invention

[0030] Use the PRECICE 600 series automatic digital slide scanning system to scan breast cancer tumor sections stained with KI67 immunohistochemistry and convert them into whole slide image data. The area with the highest proportion of KI67 positive cells was selected as the "hot spot" area, and the proportion of KI67 positive cells in the hot spot area to the tumor cells in the hot spot area was judged by computer, which was the KI67 proliferation index, and the KI67 proliferation index was selected as 15%, 20%, and 30%, respectively. %, 50%, 80% of the area, intercept the area of ​​the picture, print in color, and get 10 different standard cards.

[0031] When in use, it is only necessary to compare the K167 immunohistochemical staining section of the breast cancer to be tested with each standard comparison card to find the closest standard comparison card, and the KI67...

experiment example 1

[0034] Experimental Example 1 The effect verification of the standard comparison card of the present invention

[0035] The purpose of this experimental example is to compare the influence of the interpreter using the conventional interpretation method (method 1), the method using the conventional standard comparison card (method 2) and the method of the standard comparison card of the present invention (method 3).

[0036] The method 1 is: in the daily work of the pathologist, without any auxiliary tools, select a hotspot area in the KI67 immunohistochemically stained glass slide under the microscope, and manually interpret the proportion of KI67 positive cells, which is the KI67 proliferation index of breast cancer;

[0037] The method 2 is based on the embodiment 1, and the computer interpretation of the KI67 proliferation index is changed to manual counting, specifically:

[0038] Use the PRECICE 600 series fully automatic digital slide scanning system to scan the KI67 imm...

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Abstract

The invention relates to the field of immunohistochemical detection, and specifically discloses a method for preparing a standard comparison card for assisting in the interpretation of KI67 proliferation index, comprising the following steps: 1) A fully automatic digital section scanning system scans tumor tissue sections stained by KI67 immunohistochemistry , to obtain the image data; 2) select the hotspot area in the image data, and use the computer to interpret the proportion of KI67 positive cells in the hotspot area to the tumor cells in the hotspot area, which is the KI67 proliferation index; 3) intercept more than one known KI67 proliferation index A picture of the hotspot area. The standard comparison card of the present invention has good accuracy, is not easily affected by the seniority of doctors, and can assist doctors in stably judging the KI67 proliferation index.

Description

technical field [0001] The invention relates to the field of immunohistochemical detection. Background technique [0002] KI67 is a non-histone nuclear cortex protein that is expressed in the nucleus in the G1, S, G2 and M phases of the cell cycle, but not in the G0 phase (the quiescent state of the cell). Therefore, the proportion of cells expressing KI67 in tumor cells (also called "KI67 proliferation index") can reflect the proportion of dividing cells in tumor cells, and is often used to judge the malignancy of tumors. The higher the KI67 proliferation index, usually The higher the degree of malignancy. [0003] The KI67 proliferation index has emerged as a predictive and prognostic factor for breast cancer (BC). According to St. Gallen's guidelines, high proliferation KI67 is one of the characteristics of an increased risk of recurrence in estrogen receptor (ER)-positive HER2-negative BC, indirectly supporting the value of adding chemotherapy to endocrine therapy in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574G06T7/00
CPCG01N33/6875G01N33/57415G01N33/57496G06T7/0012G06T2207/30096G06T2207/30068
Inventor 刘月平
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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