Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof

A technology of solid medium and haploid, which is applied in the field of plant tissue culture and crop breeding, and can solve problems that have not been reported

Active Publication Date: 2010-08-18
CHINA AGRI UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0005] The technique of obtaining haploid plants by tissue cultu

Method used

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  • Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof
  • Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof
  • Method for culturing corn haploid coleoptile section tissue and specific culture medium thereof

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Experimental program
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Embodiment 1

[0045] Embodiment 1, preparation and statistical observation of corn haploid plants

[0046] 1. Obtaining haploid kernels

[0047] The maize haploid induction line Stock6 (Garst Seed Co., USA) was used as the male parent to cross with Nongda 108 (purchased from Beijing Zhongnong Dakang Technology Development Co., Ltd.). After the seeds are mature and dried, the seeds of the purple top and white embryo are selected and set aside.

[0048] 2. Obtaining and Statistical Observation of Haploid Embryo

[0049] 1. Pretreatment of haploid grains

[0050] The corn grain (being corn haploid grain of the purple top white embryo obtained in step 1, such as figure 1 As shown), pick 100 capsules, put them in an envelope, open the envelope and put it in the desiccator. Use a 250mL beaker to hold 200mL of 5.25% (volume percent) sodium hypochlorite solution, then add 2mL of concentrated hydrochloric acid to the beaker, put it in the center of the desiccator, and seal it for 8-15 hours. Ta...

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Abstract

The invention discloses a method for culturing corn haploid coleoptile section tissue and a specific culture medium thereof. The differentiation culture medium provided by the invention is the culture medium used in the differentiation and shoot formation of corn haploid coleoptile section induced callus, and is prepared by adding the following substances into MS basic culture medium: proline, casein hydrolyzate, carbon and gel, wherein the final concentration of the proline is 0.7g/L, and the final concentration of the casein hydrolyzat is 0.5g/L. The method of the invention adopts corn coleoptile section as the explant induced callus, and then differentiates the callus into a haploid strain to establish a stable and high-efficiency tissue culture system. The invention can be used for differentiation rate genetic research, material selection for tissue culture, and the like.

Description

technical field [0001] The invention relates to the fields of plant tissue culture and crop breeding, in particular to the tissue culture of maize haploid coleoptile nodes and its haploid doubling and special medium. Background technique [0002] Corn (Zea mays L.) is also known as maize, corn, corn, and cob; it is called millet in Cantonese and fanmai in Hokkien. It is an annual grass herb and the food crop with the highest total output in the world. The commercial grades are mainly divided according to the texture of the grain, which can be divided into dent, hard, silty, burst, waxy corn, sweet corn, etc. The depression at the top of the grain is caused by the unequal dryness of hard starch and soft starch in the grain. Durum corn contains less soft starch, and the top is sunken after drying. Floury corn mainly contains soft starch, which is powdery and easy to crush. Sweet corn is shriveled and transparent, and the sugar is not converted to starch. Popping corn is an...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01H4/00
Inventor 李建生杜何为刘志鹏惠国强严建兵张义荣郑艳萍
Owner CHINA AGRI UNIV
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