Fast and efficient agrobacterium-mediated wheat stem tip genetic transformation method

Abstract

The invention discloses a fast and efficient agrobacterium-mediated wheat stem tip genetic transformation method. The method mainly comprises the following steps: taking mature wheat seeds, soaking the seeds in water, performing accelerating germination, performing cold treatment, transversely cutting coleoptile, exposing stem tips to serve as transformation receptor materials, performing vacuum treatment mediation on agrobacterium, performing genetic transformation, placing the transformed materials in a suitable growth environment, and performing transgenic identification on grown plants, thereby obtaining the transgenic wheat plants. According to the method disclosed by the invention, wheat callus does not need to be cultured, influences of various factors on genetic transformation of wheat are reduced, the problems such as vitrification, browning, cell mutation and the like in the tissue culture process are solved, the conditions of genetic transformation of wheat are optimized, the economic cost of genetic transformation of wheat is reduced, restriction of wheat genotype on transformation efficiency is effectively overcome, lots of transgenic wheat plants can be rapidly obtained, and the method has significant values for cultivating high-quality new wheat varieties and performing study on genetic transformation of wheat.

Description

technical field [0001] The invention belongs to the fields of agricultural biotechnology and plant genetic engineering, in particular to a fast and efficient method for genetic transformation of wheat mediated by Agrobacterium. Background technique [0002] As one of the most important food crops in the world, wheat is one of the main sources of protein and calories for human beings. According to the prediction of the International Food Policy Research Institute, the demand for wheat in the world will grow at an annual rate of 1.6%. Urgent problems. The traditional wheat hybrid breeding process is slow and has the disadvantages of gene linkage and reproductive isolation, making it difficult to produce new quality wheat. Mutation breeding with the help of some physical and chemical conditions requires a large amount of materials, and it is difficult to control the direction of mutagenesis, the beneficial mutation rate is low, and it is difficult to screen. Obtaining hybrid...

AI Technical Summary

Problems solved by technology

Studies have shown that traditional wheat Agrobacterium genetic transformation often requires the use of plant tissue culture techniques, and the wheat receptor genotype is one of the important factors affecting the transformation efficiency of Agrobacterium. Different wheat receptor genotypes have different sensitivities to Agrobacterium. The transformation efficiency is also significantly different. Studies have shown that only some wheat genotypes have high regeneration frequency of acceptor materials, and most of the wheat with important economic value have large genotype differences and poor regeneration performance, so it is still difficult to transform. Therefore, It is of great significance to establish a wheat genetic transformation method that is less dependent on the genotype of the transformed receptor

Images