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Culture method of virus-free seedlings of curcuma wenyujin

A cultivation method, the technology of Wenyujin, which is applied in the field of cultivation of Wenyujin virus-free seedlings, can solve the problem that it is difficult to obtain safe, effective, stable and controllable Wenyujin medicinal materials, it is difficult to adapt to standardized and large-scale production, and it affects the enthusiasm and overall quality of growers. Yield and other issues, achieve good detoxification effect, solve serious vitrification, and fast reproduction

Inactive Publication Date: 2021-01-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production, Curcuma curcuma mainly uses rhizomes for reproduction, and the virus is serious, which seriously affects the yield and quality. It is difficult to obtain safe, effective, stable, and controllable curcuma curcuma. In addition, Curcuma curcuma has a long growth period and a large amount of seeds , it is difficult to adapt to the requirements of standardization and large-scale production, which has seriously affected the enthusiasm of growers and the total output

Method used

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  • Culture method of virus-free seedlings of curcuma wenyujin

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1, a kind of warm curcuma virus-free seedling culture method, carry out following steps successively:

[0061] 1), drawing materials:

[0062] In winter and spring, select the vigorously growing main rhizome of turmeric, remove the soil and fibrous roots, bury it in fine sand soaked in 0.5% carbendazim solution for 8 hours, and place it in a constant temperature box at 38±1°C to accelerate germination; until the obtained length is about 1cm strong buds;

[0063] Cut off the strong buds with a length of about 1 cm from the main rhizome, wash off the sandy soil at the base of the buds, place it in 1% (volume %) detergent solution and soak for 30 minutes, take it out, rinse it with tap water, and drain it for later use.

[0064] Note: Robust buds refer to buds that meet the following conditions: buds that grow vigorously, have a length of about 1 cm, and a diameter ≥ 0.5 cm.

[0065] 2), induction germination and differentiation of growth point:

[0066] Afte...

Embodiment 2

[0098] Example 2. Change the proliferation medium in Step 3.1) of Example 1 to: MS+6-BA 1.0 mg / L+sucrose 40 g / L+agar 8 g / L, pH 5.8. The rest are equal to Example 1.

[0099] Step 3.1) When the multiplication culture is about 40-45 days old, the multiplication factor is about 2.6.

Embodiment 3

[0100] Embodiment 3, change embodiment 1 step 4) rooting medium into: MS+6-BA3.0 mg / L+sucrose 40g / L+agar 8g / L+0.5g / L gac, pH is 5.8, all the other are equal to implementation example 1.

[0101] Step 4) After about 45 days of cultivation, the rooting rate can reach 100%, and the average number of roots is 2-3.

[0102] After testing, the detoxification rate of Examples 1 to 3 of the present invention can reach more than 80%.

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Abstract

The invention discloses a culture method of virus-free seedlings of curcuma wenyujin. The method comprises the following steps of selecting main rhizomes of curcuma wenyujin for accelerating germination until robust buds are obtained on the main rhizomes; cutting the robust buds, performing cleaning, stripping outer-layer coleoptiles, selecting growing points, and inoculating the growing points into an induced germination differentiation culture medium for culture; transferring the obtained virus-free seedlings to a proliferation culture medium for proliferation culture and repeated culture; and transferring single plantlet obtained by culture to a rooting and seedling strengthening culture medium for culture until a seedling which can be planted out of a bottle is obtained. By adopting the method disclosed by the invention, a large number of high-quality seedlings with the same genetic background and consistent growth vigor can be produced in a relatively short period, and the methodis particularly suitable for industrialized seedling production of the virus-free seedlings of the curcuma wenyujin.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating virus-free seedlings of Curcuma mellifera. Background technique [0002] Wen Yujin (Curcuma wenyujin Y.H.Chen et C.Ling) is a plant of the genus Curcuma longa in the family Zingiberaceae. It is a famous authentic medicinal material in Zhejiang Province. Curcuma has a wide range of medicinal functions. Pharmacological studies have shown that it has the effects of anti-inflammatory, analgesic, anti-tumor, anti-early pregnancy, antibacterial, increasing white blood cells, protecting the liver, inhibiting platelet aggregation and anti-thrombosis. It also exerts a series of pharmacological effects on cardiovascular, gastrointestinal smooth muscle and acute renal failure. At present, as an important medicinal material for anti-tumor, anti-virus and anti-inflammation, Curcuma curcuma is used in a huge amount in clinical and industrial production, and the supply in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 石丽敏宋费玲卢华兵许巧贤朱正梅吕学高陈一波吴志刚姜武陶正明
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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