54 results about "Curcuma wenyujin" patented technology

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

The invention discloses a curcuma wenyujin elite breeding method. The method comprises the steps of land selection and preparation, seed selection and sowing, field management, purification and rejuvenation, harvesting and seed selection, storing and transportation, wherein a region suitable for curcuma wenyujin to grow is selected for planting, base fertilizer is added in the land preparation process, the sowing time and harvesting time are shortened and standardized, early weed suppression is conducted in the mode of covering steam leaves and mulching film, the yield of curcuma wenyujin seeds is significantly increased, the quality of the curcuma wenyujin seeds is significantly improved, and the content of effective components is increased.

The invention discloses a comprehensive extraction method of Curcuma wenyujin active ingredients. The method comprises the following steps of: 1) total extraction of Curcuma wenyujin; 2) concentration of total extract; 3) extraction of beta-elemene in total extract; 4) extraction of curcumenol in total extract; 5) extraction of curcumin in total extract; 6) preparation of Curcuma wenyujin polysaccharide; 7) refining of curcumenol; 8) refining of beta-elemene. According to the invention,the active ingredients are extracted simultaneously by the comprehensive extraction method, and then, the extract is further separated and purified, the comprehensive extraction method has the advantages of high extraction rate, advanced and special technology, high daily capacity, high yield and low cost, and is suitable for industrialized production.

The invention provides a Chinese medicine for treating secondary amenorrhea. The Chinese medicine comprises the following components in part by weight: 12 to 18 parts of fluorite, 9 to 15 parts of purple salvia miltiorrhiza, 12 to 18 parts of Chinese sage herb, 3 to 7 parts of amber powder, 25 to 35 parts of Huai wheat, 8 to 12 parts of albizia julibrissin, 9 to 15 parts of platycladi seed, 9 to 15 parts of curcuma wenyujin, and 9 to 15 parts of raw tamariskoid spikemoss herb. Compared with the prior art, the Chinese medicine for treating secondary amenorrhea can treat secondary amenorrhea by differentiation of symptoms aiming at the internal pathogenic factors of secondary amenorrhea, has quick response, is low in cost, does not have toxic or side effects and can achieve effects of addressing both the symptoms and root causes.

The invention belongs to the field of pharmaceutical analysis and discloses a method for extracting pesticide from curcuma wenyujin. The method comprises the following steps that: a curcuma wenyujin medicinal material is pretreated, so that a curcuma wenyujin test solution can be obtained; a eutectic solvent is prepared; eutectic solvent functionalized graphene is prepared with the prepared eutectic solvent; dispersive micro-solid-phase extraction is performed on the curcuma wenyujin test solution with the eutectic solvent functionalized graphene adopted as an adsorbent, and an eluent is centrifuged and filtered, so that sample injection is carried out, and the pesticide enrichment effect of the curcuma wenyujin is analyzed and characterized. According to the method of the invention, the eutectic solvent functionalized graphene is adopted as the adsorbent so as to be applied to the enrichment technology of dispersive micro-solid phase extraction, and can be applied to the field of theenrichment and detection of pesticide residues in traditional Chinese medicinal materials; and the eutectic solvent is low in melting point, low in toxicity, easy to synthesize and easy to biodegrade;the surface area of the graphene is large, so that the graphene can easily adsorb and desorb various compounds; and the graphene obtained through the functional modification of the eutectic solvent has good dispersity and stability in an aqueous solution, and therefore, the reliability of the method is guaranteed.

The invention discloses an extraction and enrichment method for a sesquiterpene component in curcuma wenyujin. The sesquiterpene component is a mixture of Germacrone, Furanodiene and beta-elemene. Themethod comprises the following steps of: 1) extraction: mixing curcuma wenyujin powder and ionic liquid water solution, carrying out microwave extraction to obtain suspension, carrying out centrifugation to obtain supernatant, and carrying out filtration to obtain filtrate, so as to obtain curcuma wenyujin extracting solution; and 2) enrichment: mixing lauryl sodium sulfate, a fluorinated carbonnanotube and water, carrying out ultrasonic treatment to obtain dispersion liquid, adding the dispersion liquid into the curcuma wenyujin extracting solution, carrying out vortex and filtration aftermixing, diluting the obtained solid, and collecting the dilution liquid to obtain enrichment solution. The method is simple and low in solvent dosage, is capable of effectively avoiding the using of toxic and harmful reagents, is green and environment-friendly, is capable of avoiding the loss of the low-boiling point sesquiterpene component due to high-temperature extraction, and is suitable for large-scale popularization and application.

The invention belongs to the technical field of a traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating fatty liver. The traditional Chinese medicine composition comprises the following raw materials: 12-20 parts of Zhejiang fritillaria bulb, 10-15 parts of Curcuma wenyujin, 10-15 parts of Chrysanthemum morifolium, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of white peony root, 10-15 parts of corydalis tuber, 7-11 parts of radix scrophulariae, and 7-11 parts of Hang ophiopogon japonicus.

The invention discloses a Rhizobium sp. WYJ-E13 and its application in the preparation of a Curcuma wenyujin growth promoter. Curcuma wenyujin tissue culture seedlings grow fast and have many branchesand roots on a medium inoculated with a WYJ-13 bacterial solution, so the WYJ-13 has a Curcuma wenyujin tissue culture seedling growth promotion effect.

The invention discloses a culture method of virus-free seedlings of curcuma wenyujin. The method comprises the following steps of selecting main rhizomes of curcuma wenyujin for accelerating germination until robust buds are obtained on the main rhizomes; cutting the robust buds, performing cleaning, stripping outer-layer coleoptiles, selecting growing points, and inoculating the growing points into an induced germination differentiation culture medium for culture; transferring the obtained virus-free seedlings to a proliferation culture medium for proliferation culture and repeated culture; and transferring single plantlet obtained by culture to a rooting and seedling strengthening culture medium for culture until a seedling which can be planted out of a bottle is obtained. By adopting the method disclosed by the invention, a large number of high-quality seedlings with the same genetic background and consistent growth vigor can be produced in a relatively short period, and the methodis particularly suitable for industrialized seedling production of the virus-free seedlings of the curcuma wenyujin.

The invention discloses zedoary health-care tea and a processing method thereof. The health-care tea processed by the method is as convenient to drink as tea dust, and also has the health-care effects of aromatizing, strengthening stomach, promoting blood circulation, lowering blood pressure, resisting thrombosis, clearing liver, improving vision, strengthening body, resisting senility, and improving own immunity. The zedoary health-care tea is implemented by the technical scheme comprising the following steps of: washing Curcuma wenyujin and root of red-rooted salvia and air-drying, slicing 30 to 60 weight parts of Curcuma wenyujin, stir-frying in a pot at the temperature of between 100 and 180 DEG C by using vinegar to obtain vinegar zedoary, crushing various natural plants, namely 10 to 30 weight parts of root of red-rooted salvia, 20 to 40 weight parts of medlar, and 2 to 3 weight parts of common lophatherum herb into powder respectively to ensure that the granularity is between 0.2 and 0.6mm, fully stirring uniformly by a stirring machine, drying the powder of the natural plants by a low-temperature vacuum drying method in the prior art, loading the dried powder of the natural plants into a container and radiating, loading the processed mixed powder into filter paper tea bags, and sealing.

The invention provides an introduction cultivation and standardized planting method of curcuma wenyujin in Guizhou. The introduction cultivation and standardized planting method comprises the steps such as soil selection and preparation, seed reservation for reproduction, planting, field management, harvesting and processing as well as packaging, storage and transportation. In the introduction cultivation and standardized planting method, the curcuma wenyujin can be continuously harvested at high yield and high quality by standardizing and optimizing the steps such as planting field selection,parent ginger selection, planting method, field management as well as harvesting and seed reservation in a cultivation process of the curcuma wenyujin, so that the demand of medicine production for the curcuma wenyujin serving as a raw material is met, and meanwhile, the soil quality of a planting field cannot be affected.

The invention discloses a method for separating germacrone from Curcuma wenyujin volatile oil. The method comprises the following steps: fully mixing an aqueous solution of methyl-beta-cyclodextrin with n-hexane according to a volume ratio of 1:0.5-2, standing, carrying out liquid separation to respectively obtain an upper phase which is a mobile phase and a lower phase which is the stationary phase of a high-speed countercurrent chromatograph; and weighing the Curcuma wenyujin volatile oil, dissolving the Curcuma wenyujin volatile oil in a mixture containing the upper phase and the lower phase according to a volume ratio of 1:1, introducing the above obtained sample, carrying out high-speed countercurrent chromatographic separation, detecting through an ultraviolet detector, collecting corresponding effluents corresponding to peaks in the spectrogram of the ultraviolet detector, concentrating germacrone-containing effluents, and drying to prepare pure germacrone. The method has the advantages of simple operation, less solvent consumption, high efficiency, fastness, strong purposefulness and good separation effect.

The invention discloses a preparation method of micro-nano rhizoma zedoariae compound granules. The rhizoma zedoariae compound granules processed by the method disclosed by the invention are convenient to carry and drink and further have the health care effects of inhibiting the proliferation of connective tissues and improving the anti-tumor immunity. The invention is realized through the following technical scheme: after water-washing and air-drying are performed on curcuma wenyujin and turtle shell, the micro-nano rhizoma zedoariae compound granules are processed by the following natural plant raw materials: 10-30 parts of the curcuma wenyujin, 10-25 parts of the turtle shell, 40-60 parts of walnut kernels and 10-30 parts of honey, and the manufacturing steps are as follows: firstly respectively crushing the curcuma wenyujin, the turtle shell and the walnut kernels into powder, controlling the granularity within 0.2-0.6 micrometers, fully mixing with the honey according to the proportion, screening by a 4-8-mesh screen mesh for forming, drying by a low-temperature vacuum drying method in the prior art, then loading inn to a container for performing ultrasonic sterilization and loading particles after treatment into a bag for sealing.

Various traditional Chinese medicine components are adopted, and active components related to the medicine effect are found to be recognized difficulties in the field due to the characteristics of thesynergistic effect. The invention provides a method for screening active component groups in different processed products of curcuma wenyujin by using a bivariate correlation analysis method. The method comprises the following steps: measuring the change rule of blood entering components and drug effect indexes of different processed products of curcuma wenyujin along with time, and carrying outbivariate correlation analysis on component change and drug effect index change. The method can be used for obtaining three decoction pieces of rhizoma wenyujin longae, curcuma zedoary and vinegar-processed curcuma zedoary as well as active ingredients related to the efficacy of promoting blood circulation to remove blood stasis; although the curcuma zedoary has more blood-entering components in the three decoction pieces, the correlation with the drug effect is poorer than that of the raw curcuma zedoary and the vinegar curcuma zedoary, and the quantity of the blood-entering components in thevinegar curcuma zedoary is reduced, but the blood-entering components in the vinegar curcuma zedoary show extremely remarkable correlation with the drug effect index. According to the method providedby the invention, the relevance and similarities and differences between the blood-entering components of the three decoction pieces and the blood-activating and stasis-removing drug effects are defined, and a detailed material basis is provided for the research on the effect-taking and processing mechanism of traditional Chinese medicines, clinical practice guidance and the development of traditional Chinese medicine products.

The invention relates to a traditional Chinese medicine composition for treating cholecystitis and gall-stone. The traditional Chinese medicine composition is prepared from the following ingredients by weight: 4-6g of white mustard seed, 4-6g of raw liquorice, 4-6g of prepared polygonum multiflorum, 4-6g of tree peony bark, 4-6g of safflower, 4-6g of amomum fruit, 4-6g of yam, 4-6g of pseudostellaria root, 8-10g of anemarrhena rhizome, 8-12g of endothelium corneum gigeriae galli, 4-6g of malt, 4-6g of ligustrum lucidum, 4-6g of radix paeoniae rubra, 4-6g of asteriscus pseudosciaenae, 4-6g of curcuma wenyujin, 4-6g of raw rhubarb root, 4-6g of prepared corydalis tuber, 4-6g of cuttlebone and 4-6g of poria cocos. The traditional Chinese medicine composition has the characteristics of easiness in material obtaining, simplicity in preparation and low cost, is convenient in use, low in content of harmful ingredients and remarkable in curative effect and is free from toxic or side effects.

The invention provides a new sesquiterpene compound separated from curcuma wenyujin, as well as a preparation method and the medical application of the new sesquiterpene compound. The invention discloses the new sesquiterpene compound. The preparation method of the compound is implemented by use of a high-pressure airtight steaming process so as to prevent the compound from being brought away by water vapor, and therefore, the yield of the compound is increased. The new sesquiterpene compound can be used an active ingredient for preparing anticoagulant and antithrombotic drugs.

The invention relates to the field of medicine formulae, and in particular relates to a traditional Chinese medicine for treating cardiovascular neurosis. The traditional Chinese medicine is characterized by being prepared from the following raw materials in parts by weight: 5-9 parts of nacre mother of pearl, 4-6 parts of vine of multiflower knotweed, 6-10 parts of magnets, 4-7 parts of amethyst, 2-4 parts of rhizoma acori graminei, 2-3 parts of allium macrostemon, 2-4 parts of atractylodes macrocephala, 3-5 parts of dalbergia wood, 2-4 parts of citron, 1-2 parts of sandalwood, 2-4 parts of curcuma aromatica, 2-5 parts of dianthus superbus, 4-7 parts of rainbow conk, 2-5 parts of Chinese roses, 2-4 parts of tabasheer, 3-5 parts of longan aril, 3-6 parts of lotus seeds, 1-2 parts of kadsura longepedunculata, 2-4 parts of platycladi seeds, 5-8 parts of black beans, 3-5 parts of trichosanthes kirilowii, 2-4 parts of gastrodia elata, 3-5 parts of poria cocos, 2-5 parts of spina date seeds, 1-2 parts of amber and 2-3 parts of curcuma wenyujin. The raw material medicines are mixed and soaked and boiled with water, and the residues are removed so as to obtain the filtrate. The traditional Chinese medicine provided by the invention is high in treatment effect in treating cardiovascular neurosis, is free of toxic or side effect, has the effects of tonifying heart and smoothening the nerves, promoting blood circulation to remove blood stasis, warming the body and activating yang, activating qi and blood, moistening and nourishing the myocardium, tonifying qi and nourishing yin, nourishing the liver and the kidney, activating meridians to stop pain and accepting qi and relieving asthma, is applicable to various cardiovascular neurosis diseases, and has the effect of treating both the symptoms and the root causes.

The invention discloses an alpha-santalene synthase, a gene and application. The alpha-santalene synthetase is derived from Curcuma wenyujin Y.H.Chen et C.Ling, and the amino acid sequence of the alpha-santalene synthetase is as shown in SEQ ID NO. 2. Functional verification is carried out on alpha-santalene synthetase from curcuma wenyujin, and an alpha-santalene synthesis method is invented and applied to escherichia coli. According to the present invention, it is found that the curcuma wenyujin-sourced alpha-santalene synthase can catalyze FPP in a prokaryotic expression system to generate a large amount of alpha-santalene and a small amount of beta-santalene and alpha-farnesene, and the content of the alpha-santalene calculated according to the peak area achieves more than 90%, such that the catalyst can be rapidly and massively prepared in Escherichia coli and be applied to synthesis of alpha-santalene, and the catalyst can be further applied to synthesis of alpha-santalol and has important significance.

The invention discloses traditional Chinese medicine oral cavity refreshing liquid. The traditional Chinese medicine oral cavity refreshing liquid is prepared from Arabis pendula, echinops sphaerocephalus, anredera cordifolia leaves, panax pseudoginseng, curcuma wenyujin, radix trichosanthis, manyleaf paris rhizomes, the bark of ash, stir-fried hyacinth beans, lepidium sativum, rorippa globosa, costus roots, Japonese pearlwort herb, hedyotis diffusa, xylooligosaccharide, glycyrrhizic acid and glycyrrhizic acid. The traditional Chinese medicine oral cavity refreshing liquid is rich in raw material and fast in curative effect, and the effective rate reaches 100%. The raw materials are easy to obtain, the price is low, and the economical burden of a patient is relieved; baicalein is extracted purely naturally, safety and health are achieved, toxic and side effects are avoided, the extraction rate reaches up to 22.1%, and the utilization rate is increased; all the traditional Chinese medicine is extracted and purified many times, the yield is increased by 13.4%, and a large amount of production cost is reduced; the taste is fragrant and slightly sweet, the foreign taste of the traditional Chinese medicine is avoided, the patient has no contradicting feeling, use is easy, the traditional Chinese medicine oral cavity refreshing liquid is not limited to the oral cavity focus, the functions of all organs can be adjusted, organism toxins are removed, bacterium resistance and inflammation resistance are achieved, the breath is refreshed fundamentally, and the traditional Chinese medicine oral cavity refreshing liquid is effective durably.

The invention discloses an induction method of hairy roots of Curcuma wenyujin. The induction method comprises the following steps: (1) preparation of infecting liquid, (2) culture of living tissue-cultured seedlings, (3) induction of the hairy roots, and (4) bacteria-free culture and reproduction of the hairy roots. According to the induction method, the tissue-cultured seedlings are infected byvirtue of endophytes of the Curcuma wenyujin and are taken as receptors of Agrobacterium rhizogenes, and stems of the living tissue-cultured seedlings of the Curcuma wenyujin are infected by virtue ofthe living Agrobacterium rhizogenes so as to effectively induce the hairy roots, so that a technical support is provided for growing medicinal components by virtue of a hairy root induction system ofthe Curcuma wenyujin.

The invention discloses a curcuma wenyujin tissue culture method. The method mainly includes steps of tuber sand-bed germination accelerating, adventitious bud induction, subculture multiplication, rooting culture, heeling-in planting and the like. By adoption of the method, in a culture process, the contamination rate is smaller than 5%, the adventitious bud induction rate reaches 129% or above,the bud proliferation rate reaches 7.5 or above, the survival rate reaches 95% or above, and the volatile oil content reaches 5.4% or above. The method is simple and convenient to operate and remarkably effective, a great quantity of high-quality curcuma wenyujin seedlings can be provided in short time, and the method is suitable for industrial popularization and utilization.