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Preparation methods of radix curcumae wenyujin agglutinin crude product, radix curcumae wenyujin agglutinin pure product and PGRP bacterial strain mixture, and radix curcumae wenyujin fertilizer

A strain mixture and the technology of warming turmeric, applied to the preparation method of peptides, organic fertilizers, chemical instruments and methods, etc., can solve the problems of less lectin and unfavorable production utilization, etc., and achieve simple preparation process, convenient industrial production, and low cost low effect

Active Publication Date: 2019-04-02
合肥市未来药物开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in previous experiments, very little lectin was isolated, which is not conducive to production utilization

Method used

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  • Preparation methods of radix curcumae wenyujin agglutinin crude product, radix curcumae wenyujin agglutinin pure product and PGRP bacterial strain mixture, and radix curcumae wenyujin fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 1 kg of fresh turmeric, slice it, crush it and beat it to get the slurry raw material, centrifuge it at 4°C and 9500r / min for 35min, discard the supernatant, and get the first sediment;

[0023] (2) Add 1~3% compound enzyme solution to the phosphate buffer solution with pH of 5.5~6.5, the compound enzyme solution is cellulase: pectinase=2.5:1, and the first sediment Mix at a volume ratio of 6:1, stir at room temperature for 5 hours at low speed after mixing, then centrifuge at 4°C and 2000 r / min for 10 minutes, take the supernatant, and then centrifuge at 4°C and 9500 r / min for 35 minutes. Discard the supernatant to obtain the second precipitate;

[0024] (3) Add pre-cooled petroleum ether to the second sediment at a volume ratio of 1:1, stir at a low speed for 25 minutes at 4°C, filter with suction, add pre-cooled acetone at a volume ratio of 1:0.7, and let stand for 1 -2min, suction filtration, and air-dried to obtain the third precipitate;

[0025] (4) Add...

Embodiment 2

[0029] (1) Take 1 kg of fresh turmeric, slice it, crush it and beat it to get a pulpy raw material, centrifuge it at 4°C and 8000r / min for 40min, discard the supernatant, and get the first sediment;

[0030] (2) Add 1~3% compound enzyme solution to the phosphate buffer solution with pH of 5.5~6.5, the compound enzyme solution is cellulase: pectinase=2:1, and the first sediment Mix at a volume ratio of 5:1. After mixing, stir at a low speed for 5.5 hours at room temperature, then centrifuge at 4°C and 2000 r / min for 10 minutes, take the supernatant, and then centrifuge at 4°C and 8000 r / min for 40 minutes. Discard the supernatant to obtain the second precipitate;

[0031] (3) Add pre-cooled petroleum ether to the second sediment at a volume ratio of 1:2, stir at a low speed for 20 minutes at 4°C, filter with suction, add pre-cooled acetone at a volume ratio of 1:0.7, and let stand for 1 -2min, suction filtration, and air-dried to obtain the third precipitate;

[0032] (4) Add...

Embodiment 3

[0036] (1) Take 10kg of fresh curcuma, slice, crush and beat to obtain slurry raw material, centrifuge at 4°C and 10000r / min for 45min, discard the supernatant, and obtain the first sediment;

[0037] (2) Add 1~3% compound enzyme solution to the phosphate buffer solution with pH of 5.5~6.5, the compound enzyme solution is cellulase: pectinase=3:1, and the first sediment Mix at a volume ratio of 7:1, stir at room temperature for 5.5 hours at low speed after mixing, then centrifuge at 4°C and 2000 r / min for 10 minutes, take the supernatant, and then centrifuge at 4°C and 10000 r / min for 45 minutes. Discard the supernatant to obtain the second precipitate;

[0038] (3) Add pre-cooled petroleum ether to the second sediment at a volume ratio of 1:2, stir at a low speed for 20 minutes at 4°C, filter with suction, add pre-cooled acetone at a volume ratio of 1:0.7, and let stand for 1 -2min, suction filtration, and air-dried to obtain the third precipitate;

[0039] (4) Add phosphat...

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Abstract

The invention relates to preparation methods of a radix curcumae wenyujin agglutinin crude product, a radix curcumae wenyujin agglutinin pure product and a PGRP bacterial strain mixture, and a radix curcumae wenyujin fertilizer. According to the preparation methods disclosed by the invention, sequentially through mashing, enzymolysis, degreasing, ammonium sulfate precipitation, dialysis and freezedrying, a high-activity radix curcumae wenyujin agglutinin crude product is obtained, and through agglutination between radix curcumae wenyujin agglutinin and specific PGRP bacterial strains, purification of the radix curcumae wenyujin agglutinin and the specific PGRP bacterial strains is realized. The obtained specific PGRP bacterial strain mixture can be used for increasing production of curcuma wenyujin. According to a separation method provided by the invention, the obtained radix curcumae wenyujin agglutinin crude product is high in activity. The radix curcumae wenyujin specific PGRP bacterial strain mixture prepared from the radix curcumae wenyujin agglutinin crude product is used for breeding the curcuma wenyujin, so that obvious yield increment of the curcuma wenyujin can be realized, and the preparation process is simple, low in cost and convenient for industrial production.

Description

technical field [0001] The invention relates to a preparation method of crude and pure turmeric lectin, a mixture of PGRP strains and a turmeric lectin fertilizer. Background technique [0002] Curcuma wenyufin, a plant of the ginger family, is a famous medicinal material in Zhejiang Province. The turmeric oil extracted from it has anti-virus and anti-tumor effects.... Limited by environmental factors such as temperature and light, the cultivation of Wen Yujin is limited to Wenzhou area. Due to the limited area, farmers have been planting continuously in the same field for a long time. This planting method leads to continuous cropping obstacles, resulting in reduced yield, poor quality, poor reproductive ability, and reduced content of effective medicinal substances of Curcuma curcuma, resulting in serious economic losses. [0003] Plant growth-promoting rhizobacteria (PGPR) refers to a class of beneficial fungi that live freely in the soil or epiphyte in the rhizosphere of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/42C07K1/30C07K1/14C12N1/20C05F11/08
CPCC05F11/08C07K14/42C12N1/20
Inventor 姜程曦袁玮赵祺任仙樱鲍康德秦宇雯李帆张金华郭月琴罗莎洪涛
Owner 合肥市未来药物开发有限公司
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