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Curcuma wenyujin tissue culture method

A tissue culture and warm turmeric technology, applied in the field of plant tissue culture, can solve the problems of poor induction and proliferation effect, and achieve the effect of reducing the pollution rate and increasing the multiplying multiple.

Active Publication Date: 2019-12-24
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant tissue culture technology is a method of rapid propagation using explants such as roots, stems, leaves, flowers, and fruits of plants, which can effectively make up for the shortcomings of traditional cultivation methods. Many deficiencies, for example, poor induction and proliferation effects, etc., therefore, urgently need to seek a method more suitable for tissue culture of Wen Yujin

Method used

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  • Curcuma wenyujin tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for tissue culture of turmeric, comprising the following steps:

[0036] S1: Germination of tubers in sand bed

[0037] Take healthy tubers and spread them neatly on clean and fresh river sand, keep the sand bed moist, and after one month, several seedlings will grow on the tubers;

[0038] S2: Explant acquisition

[0039] Select healthy seedlings, cut off the rhizome, disinfect with 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 20 minutes, wash with sterilized water for 3 to 5 times, and inoculate in the seedlings containing 30g / L sucrose and 5g / L karaage. Glue, MS medium of 5mg / L6-BA, to obtain sterile explants;

[0040] S3: Induction of adventitious buds

[0041] When the buds grow to 3-5cm, inoculate the buds in MS medium containing 30g / L sucrose, 5g / L carrageenan, 5mg / L6-BA and 0.5mg / L NAA to induce culture, and pick one bud per bottle ; The pH of the medium is 5.5-5.8. After 4-5 days, observe the growth of the buds, pick u...

Embodiment 2

[0050] A method for tissue culture of turmeric, comprising the following steps:

[0051] S1: Germination of tubers in sand bed

[0052] Take healthy tubers and spread them neatly on clean and fresh river sand, keep the sand bed moist, and after one month, several seedlings will grow on the tubers;

[0053] S2: Explant acquisition

[0054]Select healthy seedlings, cut off the rhizome, disinfect with 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 20 minutes, wash with sterilized water for 3 to 5 times, and inoculate in the seedlings containing 35g / L sucrose and 6g / L karaage. Glue, MS medium of 4mg / L6-BA, to obtain sterile explants;

[0055] S3: Induction of adventitious buds

[0056] When the buds grow to 3-5cm, inoculate the buds in MS medium containing 35g / L sucrose, 6g / L carrageenan, 2mg / L 6-BA and 1.0mg / L NAA to induce culture, and pick one bud per bottle ; The pH of the medium is 5.5-5.8. After 4-5 days, observe the growth of the buds, pick u...

Embodiment 3

[0065] The difference between embodiment 3 and embodiment 1 is:

[0066] The MS medium described in step S3 also contains 3 g / L fucoidan, 6.5 mg / L salicylic acid and 11 mg / L methyl jasmonate, and the calcium chloride concentration in the medium is changed to 640 mg / L.

[0067] The MS medium described in step S5 also contains 5 mg / L methyl jasmonate, and the concentration of calcium chloride in the medium is changed to 520 mg / L.

[0068] From step S1 to step S4, under the condition of red and blue LED light for 10 hours a day, the ratio of red and blue light is 4:1, the light intensity is 1600-1800lx, the ambient humidity is 40-60%, and the ambient temperature is 25-28°C; step S5, under the white light condition Illumination for 10 hours a day under white light conditions, light intensity of 1800-2200lx, ambient humidity of 40-60%, and ambient temperature of 25-28°C; step S6, under white light conditions for 10 hours of light per day, light intensity of 1800-2200lx, and ambient...

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Abstract

The invention discloses a curcuma wenyujin tissue culture method. The method mainly includes steps of tuber sand-bed germination accelerating, adventitious bud induction, subculture multiplication, rooting culture, heeling-in planting and the like. By adoption of the method, in a culture process, the contamination rate is smaller than 5%, the adventitious bud induction rate reaches 129% or above,the bud proliferation rate reaches 7.5 or above, the survival rate reaches 95% or above, and the volatile oil content reaches 5.4% or above. The method is simple and convenient to operate and remarkably effective, a great quantity of high-quality curcuma wenyujin seedlings can be provided in short time, and the method is suitable for industrial popularization and utilization.

Description

technical field [0001] The invention relates to a method for tissue culture of warm turmeric, belonging to the technical field of plant tissue culture. Background technique [0002] Wenyujin (Curcuma wenyujin Y.H.Chen et C.Ling), also known as Wenzezhu, belongs to Zingiberaceae Curcuma, perennial bulbous herbaceous flowers, plant height 100-120cm, clustered plants, oblong leaves, bright green, can be used for foliage viewing Ornamental plants, suitable for garden cultivation or as large potted plants. The flowering period is from March to May. Cylindrical spikes emerge from the rhizomes, and the purple-red bracts are arranged tightly on the inflorescences. The flowering period lasts for a long time and can be used as flower mirrors and flower beds. The properties of Wen Yujin are round or irregular flakes, 1.5-4cm in diameter, gray-yellow surface, yellow-brown to brown-brown cut surface, often with yellow-white or yellow-brown powder, and many and obvious vascular bundle ma...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郑丽谢昌平周佳徐刚王渝华仇芳李希
Owner HAINAN UNIVERSITY
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