Tissue culture method for regenerated plants of curcuma wenyujin

A technology for plant regeneration and tissue culture, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long regeneration process, tissue culture pollution, and low regeneration frequency, and achieve the effect of overcoming easy pollution.

Active Publication Date: 2017-12-01
HANGZHOU NORMAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to provide a pollution-free and efficient method for rapidly propagating Curcuma curcuma, which overcomes the problems of serious pollution in tissue culture of Curcuma curcuma, low regeneration frequency, and long regeneration process time, and is beneficial to the variety improvement of Curcuma curcuma in production practice.

Method used

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  • Tissue culture method for regenerated plants of curcuma wenyujin
  • Tissue culture method for regenerated plants of curcuma wenyujin
  • Tissue culture method for regenerated plants of curcuma wenyujin

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Embodiment 1

[0022] 1. Selection of explants:

[0023] Utilize robust warm turmeric roots, bury them in the culture soil prepared with peat: vermiculite = 4:1 (v / v), keep the culture soil moist, 30-40 days at 20-25°C, wait for the roots to grow 1-1.5cm long bud tip, cut the bud tip from the place 0.1-0.2cm away from the tuber as the bud tip segment and be the explant.

[0024] Then take the root tubers of Curcuma mellifera and the stem segments and young leaves at the top of the seedlings of Curcuma mellifera grown in the field as explants.

[0025] 2. Disinfection of explants

[0026] After each kind of explant was taken off, it was soaked for 10 minutes with a tap water cleaning solution containing a small amount of detergent (detergent / tap water (v / v)=1 / 1000), then rinsed with tap water for 3 times, and finally transferred to Disinfection in different disinfectants (Table 1, diluting antiformin 5 times is the mass concentration of 6% sodium hypochlorite aqueous solution). The sterili...

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Abstract

The invention discloses a tissue culture method for regenerated plants of curcuma wenyujin. The tissue culture method includes inoculating disinfected explants into cluster bud culture media and culturing the explants under the conditions of the temperatures of 20-25 DEG C and the illumination of 1000-1200 lux so as to obtain cluster buds; cutting the cluster buds to obtain individuals with terminal buds, inoculating the individuals in rooting culture media and culturing the individuals under the conditions of the temperatures of 20-25 DEG C and the illumination of 1000-1200 lux so as to obtain the complete plants with roots. The tissue culture method has the advantages that optional pollution is prevented in explant culture procedures, and accordingly the severe problem of previously reported easiness in pollution due to curcuma wenyujin culture can be solved; procedures for forming the cluster buds and the complete regenerated plants are speedy and are high in efficiency; large quantities of cluster buds can be obtained after the explants are cultured for 25-30 days, and the complete plants with robust root systems can be obtained after the cluster buds are cut and then are cultured on the rooting culture media for 15-20 days.

Description

(1) Technical field [0001] The invention relates to a method for tissue culture and regenerated plants of warm turmeric. (2) Background technology [0002] Wenyujin (Curcuma wenyujin Y.H.Chen et C.Ling), also known as Wenyujin and Black Curcuma, is mainly produced in Ruian, Wenzhou, Zhejiang. Curcuma curcuma is an authentic medicinal material in Zhejiang. Its nature and flavor are pungent, bitter, and warm, and it returns to the liver and spleen meridian. It has the effects of promoting qi, breaking blood, eliminating accumulation and relieving pain. Modern pharmacological studies have shown that turmeric has antitumor, antithrombotic, antibacterial, and antiviral effects, and has good curative effects on the respiratory system, digestive system, genitourinary system, circulatory system, and skin ulcers. Because of its wide range of medicinal functions, it is widely used in clinical treatment of traditional Chinese medicine. [0003] Wen Yujin mainly reproduces by rhizomes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 向太和冯咪李蓉李亚菲王蕾臻金婷婷王克云
Owner HANGZHOU NORMAL UNIVERSITY
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