Tissue culture method for regenerated plants of curcuma wenyujin
A technology for plant regeneration and tissue culture, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long regeneration process, tissue culture pollution, and low regeneration frequency, and achieve the effect of overcoming easy pollution.
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[0022] 1. Selection of explants:
[0023] Utilize robust warm turmeric roots, bury them in the culture soil prepared with peat: vermiculite = 4:1 (v / v), keep the culture soil moist, 30-40 days at 20-25°C, wait for the roots to grow 1-1.5cm long bud tip, cut the bud tip from the place 0.1-0.2cm away from the tuber as the bud tip segment and be the explant.
[0024] Then take the root tubers of Curcuma mellifera and the stem segments and young leaves at the top of the seedlings of Curcuma mellifera grown in the field as explants.
[0025] 2. Disinfection of explants
[0026] After each kind of explant was taken off, it was soaked for 10 minutes with a tap water cleaning solution containing a small amount of detergent (detergent / tap water (v / v)=1 / 1000), then rinsed with tap water for 3 times, and finally transferred to Disinfection in different disinfectants (Table 1, diluting antiformin 5 times is the mass concentration of 6% sodium hypochlorite aqueous solution). The sterili...
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