Application of curcuma wenyujin diterpene compound C in drugs for treating gastrointestinal inflammation
A technology of warming turmeric and compounds is applied in the application field of preparing medicines for treating gastrointestinal inflammation, and achieves the effects of inhibiting inflammation and broadening the scope of medicine selection.
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Embodiment 1
[0020] The preparation of embodiment 1 warm turmeric diterpenoid C
[0021] Warm 10 kg of dried rhizomes of turmeric, extract 4 times with 8 times the amount (80 L) of 95% ethanol under reflux, and recover the solvent to obtain 247 g of crude extract. The total extract was uniformly dispersed with 500mL of water, extracted with 1 times the amount (500mL) of petroleum ether, dichloromethane, and n-butanol in turn to obtain 95.1g of dichloromethane extract, and the dichloromethane part was based on petroleum ether / ethyl acetate. Eluent (volume ratio: 100:0, 100:10, 100:20, 100:30, 100:40, 100:50, 100:60, 100:70, 100:80, 100:90), silica gel Column chromatography obtained 10 fractions A, B, C, D, E, F, G, H, I, J. Fraction E uses acetonitrile (70%)-water (30%) as the mobile phase, and is further separated by preparative chromatography to obtain 8 fractions (0-80min, 1 fraction per 10 minutes), namely E1-E8, wherein E8 is separated by acetonitrile (45%)-water (55%) was eluted iso...
Embodiment 2
[0022] Carrying out 2 structure identification of diterpenoid C in curcuma
[0023] White needle crystals prepared according to Example 1, HREIMS M + 380.2581, the molecular formula is determined to be C 22 h 36 o 5 (calculated M380.2563), its unsaturation is 5, ESI-MS (negative) gives m / z: 319[M-H] - .
[0024] 13 The C-NMR spectrum shows that there are 22 carbons, and the structure contains two carbonyl carbons from δ208.9 and δ170.4; δ70.4, δ76.0 and δ81.6 are three oxycarbon signals; δ111.4 and δ145.8 is two olefinic carbon signals; combined with the degree of unsaturation, it is speculated that the structure may contain two rings.
[0025] from 1 From the H-NMR spectrum, there are six consecutive quaternary carbon methyl proton signals: δ0.83 (3H, s), 0.86 (3H, s), 0.94 (3H, s), 1.22 (3H, s), 1.47 (3H, s) and δ2.13(3H, s); δ5.36(1H, m) suggested an oxo-methine signal; δ5.05(1H, d, J=12.0Hz), 5.10(1H , d, J=18.0Hz) and δ6.06 (1H, dd, J=12.0, 18.0Hz) are the three ...
Embodiment 3
[0036] Example 3 Application of warm turmeric diterpenoid C on inhibition of gastrointestinal inflammation
[0037] (1) Cell culture
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