Curcuma wenyujin detoxicating and quick reproduction method

A kind of warm and fast technology, applied in the field of detoxification and rapid reproduction of warm turmeric, to achieve the effect of simple equipment, small footprint, and easy factory production.

Inactive Publication Date: 2008-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] So far, there have been no reports on the detoxifica...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The rhizomes of turmeric curcuma collected from Ruian, Wenzhou were placed at 36°C for 5 weeks, the rhizomes were taken out, soaked in 400 times chlorothalonil for 30 minutes, and placed in a large plastic turnover box (with a few small holes in the bottom) , with fine sand as the bottom and covering (watering), the turnover box is placed in a greenhouse environment (27 ° C) for germination and cultivation for 20 days (regular water spray). When the buds to be cultivated grow to 1-2 cm, select the terminal buds and axillary buds for surface sterilization: wash with running water for 40 minutes, place them in 75% alcohol for 20 seconds, and then use 0.1% HgCl 2 (adding 2 drops of Tween T-20 inside) sterilized for 6 minutes and rinsed with sterile water for 3 times before peeling and inoculating the shoot tip. Take the sterilized 1-2 cm buds, peel off the shoot tip growth point (with a leaf primordium) under a 40 times stereo microscope, cut into small pieces of 0.1-0.2 m...

Embodiment 2

[0052] The rhizomes of Curcuma curcuma collected from Ruian, Wenzhou were placed at 38°C for 4 weeks, the rhizomes were taken out, soaked in 500 times chlorothalonil for 40 minutes, and placed in a large plastic turnover box (with a few small holes in the bottom) , with fine sand as the base and covering (watering), the turnover box was placed in a greenhouse environment (23 ° C) for germination and cultivation for 27 days (regular water spray). When the buds to be cultivated grow to 1-2 cm, transfer the rhizomes and their germinated buds to a light incubator at 37°C for 24 hours to cultivate for 24 hours to weaken the virus, and then select terminal buds and axillary buds for surface sterilization: After rinsing with running water for 30 minutes, place in 75% alcohol for 30 seconds, and then use 0.1% HgCl 2 (Add 3 drops of Tween T-20 inside) After sterilizing for 10 minutes, rinse with sterile water for 5 times and then use it for the stripping and inoculation of the shoot ti...

Embodiment 3

[0054] The rhizomes of Curcuma curcuma collected from Ruian, Wenzhou were placed at a temperature of 37°C for 4 weeks, the rhizomes were taken out, soaked in 400 times chlorothalonil for 30 minutes, and placed in a large plastic turnover box (with a few small holes in the bottom) , use fine sand to lay the bottom and cover (water thoroughly), place the turnover box in a greenhouse environment (25°C) for germination and culture for 25 days (regularly spray water). When the buds to be cultivated grow to 1-2 cm, transfer the rhizomes and their germinated buds to a light incubator at 37°C for 24 hours to cultivate for 24 hours to weaken the virus, and then select terminal buds and axillary buds for surface sterilization: After rinsing with running water for 30 minutes, place in 75% alcohol for 30 seconds, and then use 0.1% HgCl 2(adding 2 drops of Tween T-20 inside) sterilized for 8 minutes and rinsed with sterile water 4 times for the stripping and inoculation of the shoot tip. ...

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PUM

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Abstract

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detoxification and rapid propagation of turmeric. Background technique [0002] Plants are a natural treasure house of medicines, and humans have used medicinal plants to prevent and treat diseases for thousands of years. About 75% of the world's population uses plants as a source of medicine for treating diseases (Xing Jianmin, 2001). About 25% of today's prescription drugs come from medicinal plants (Qiu Deyou, 2000). With the rapid development of the economy and medical care, the demand for traditional Chinese medicine resources has increased unprecedentedly, and the research on extracting active ingredients from traditional Chinese medicine for cosmetics, food and industrial raw materials has become more and more in-depth. These reasons have increased to varying degrees. Increased pressure on traditional Chinese medicine resources. In addition, for a long time, th...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 姜维梅沈瑛瑛傅承新
Owner ZHEJIANG UNIV
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