Curcuma wenyujin detoxicating and quick reproduction method

Abstract

The invention discloses a detoxification, tissue culture and rapid propagation method of common turmeric, which comprises the explant selection and treatment, induction culture, the virus identification of plant regeneration, proliferation culture, transplanting and other steps. In the invention, on the basis of the MS culture medium, the plant growth regulators such as 6-benzyladenine, kinetin, Alpha-banai acid, adenine sulphate are added for the induction culture of common turmeric apical meristem and the proliferation and rooting of multiple shoot two key stages. With the adoption of the packaged technology and method provided in the invention for the tissue culture and rapid propagation of common turmeric, the difficult problems of common turmeric such as virus accumulation, declining quality and yield reduction caused by propagation coefficient and technical stability are met. Therefore, the invention can provide a seedling-raising method with short cycle, high rate of reproduction, low-cost for the factory production of common turmeric.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for detoxification and rapid propagation of turmeric. Background technique

[0002] Plants are a natural treasure house of medicines, and humans have used medicinal plants to prevent and treat diseases for thousands of years. About 75% of the world's population uses plants as a source of medicine for treating diseases (Xing Jianmin, 2001). About 25% of today's prescription drugs come from medicinal plants (Qiu Deyou, 2000). With the rapid development of the economy and medical care, the demand for traditional Chinese medicine resources has increased unprecedentedly, and the research on extracting active ingredients from traditional Chinese medicine for cosmetics, food and industrial raw materials has become more and more in-depth. These reasons have increased to varying degrees. Increased pressure on traditional Chinese medicine resources. In addition, for a long time, th...