Rhizobium sp. WYJ-E13 and its application in preparation of Curcuma wenyujin growth promoter
A WYJ-E13, growth promoter technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of easy infection of viruses, pests and diseases, etc., to achieve more branches, more roots, and promote growth. Effect
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[0019] 1. Materials and methods
[0020] 1. Experimental materials: Wenyujin, cultivar Wenyujin No. 1, donated by Zhejiang Wenzhou Tianhe Biotechnology Co., Ltd. Wen Yujin is planted in the glass house of the School of Life and Environmental Sciences, Hangzhou Normal University.
[0021] 2. Disinfection of root tubers and isolation of endophytes
[0022] For seedlings grown for 5 months (for seedlings, see Example 1 of patent application 2017108783535), take elliptical roots with a diameter of about 1-2 cm, rinse them with tap water, soak them in 75% alcohol for 1 min, and then use 0.1% mercury liter Disinfect for 15 minutes, and finally rinse with sterile water 3 times. Cut the root tubers into small pieces of 3-5 mm, place them on the potato dextrose agar (PDA) medium with or without Str (50 mg / L), with the cut side facing the medium, and place 4 pieces in each dish. The above-mentioned treated root tubers were directly rolled on the control plate without cutting to test ...
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