Rhizobium sp. WYJ-E13 and its application in preparation of Curcuma wenyujin growth promoter

A WYJ-E13, growth promoter technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve the problems of easy infection of viruses, pests and diseases, etc., to achieve more branches, more roots, and promote growth. Effect

Active Publication Date: 2018-08-24
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wen Yujin mainly reproduces by rhizomes, but it is susc...

Method used

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  • Rhizobium sp. WYJ-E13 and its application in preparation of Curcuma wenyujin growth promoter
  • Rhizobium sp. WYJ-E13 and its application in preparation of Curcuma wenyujin growth promoter
  • Rhizobium sp. WYJ-E13 and its application in preparation of Curcuma wenyujin growth promoter

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Embodiment 1

[0019] 1. Materials and methods

[0020] 1. Experimental materials: Wenyujin, cultivar Wenyujin No. 1, donated by Zhejiang Wenzhou Tianhe Biotechnology Co., Ltd. Wen Yujin is planted in the glass house of the School of Life and Environmental Sciences, Hangzhou Normal University.

[0021] 2. Disinfection of root tubers and isolation of endophytes

[0022] For seedlings grown for 5 months (for seedlings, see Example 1 of patent application 2017108783535), take elliptical roots with a diameter of about 1-2 cm, rinse them with tap water, soak them in 75% alcohol for 1 min, and then use 0.1% mercury liter Disinfect for 15 minutes, and finally rinse with sterile water 3 times. Cut the root tubers into small pieces of 3-5 mm, place them on the potato dextrose agar (PDA) medium with or without Str (50 mg / L), with the cut side facing the medium, and place 4 pieces in each dish. The above-mentioned treated root tubers were directly rolled on the control plate without cutting to test ...

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Abstract

The invention discloses a Rhizobium sp. WYJ-E13 and its application in the preparation of a Curcuma wenyujin growth promoter. Curcuma wenyujin tissue culture seedlings grow fast and have many branchesand roots on a medium inoculated with a WYJ-13 bacterial solution, so the WYJ-13 has a Curcuma wenyujin tissue culture seedling growth promotion effect.

Description

(1) Technical field [0001] The invention relates to a beneficial microorganism that is symbiotic with plants as a plant growth-promoting bacterium and an application method thereof. (2) Background technology [0002] Curcuma is a plant resource with wide application value. It has a beautiful flower shape and can be used as an ornamental plant for potting or greening; it also has the effect of preventing and treating various tumors. Its main active ingredients are volatile oil and curcumin; as a natural and safe food pigment, curcumin is widely used in food additives. In particular, studies in recent years have shown that elemene, a medicinal ingredient isolated from turmeric, has special anti-tumor effects. Wen Yujin mainly reproduces by rhizomes, but it is susceptible to viruses and is harmed by various pests and diseases. On the other hand, there are a variety of endophytes in plants, and the secondary metabolites of endophytes form a mutualistic relationship with the h...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/41
CPCA01N63/10C12N1/20C12N1/205C12R2001/41
Inventor 向太和童霞秀王蕾臻李亚菲王克云李蓉金婷婷徐正贤
Owner HANGZHOU NORMAL UNIVERSITY
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