33 results about "Germacrone" patented technology

A formulation, method and system for the topical, transdermal or transmucosal administration of a therapeutically effective active agent. Particularly, the invention provides a formulation, system and method for enhancing the permeation or penetration of active agents across the dermal or mucosal surfaces of a mammalian subject. The formulation includes a plant extract of the genus Curcuma of the family Zingiberaceae, a germacrone, or a natural or synthetic constituent thereof, which has been found to increase penetration of the active agent across the dermal or mucosal surface. If desired, a secondary permeation enhancer of a polyalcohol, a monoalkyl ether of diethylene glycol, a tetraglycol, or a mixture thereof can be used for certain active agents for optimal permeation enhancement.

The present invention relates to A composition obtained from the lipid soluble extract of rhizomes and leaves of Curcuma species of Zingiberaceae family, useful for the treatment of neurocerebrovascular disorders, said composition comprising fraction A consisting of ar-turmerone of formula 1, and turmerone of formula 2, and / or along with fraction B consisting of curcumene and zingiberine, and / or fraction C consisting of germacrone, curcumerone, zedoarone, sedoarondiol, isozdedoaronidiol, curcumenone, and curlone, and / or pharmaceutically acceptable additives and a method of treating neurocerebrovascular disorders in animals including humans using said composition by administering therapeutically effective amount of lipid soluble extract.

The invention discloses a high quality and yield culture technology of curcuma zedoaria, which comprises the following steps: land selection and preparation, seed curcuma preparation, sowing, field management, harvesting and prevention and cure of plant diseases and insect pests. The technology of the invention is characterized in that: the rotation land or clay with neutral PH or micro acid in which curcuma zedoaria is not planted in the previous year is selected; prior to sowing, the rootstalk with two or three heads which has a diameter of more than 20mm and a length of more than 50mm, or a big curcuma which has the diameter of more than 15mm and the length of more than 70mm is selected as the seed curcuma, and the seed curcuma is sowed from May 20 to May 30 in a normal year; the planting density is 2700-3300 plants per mu with the row spacing of 0.45-0.50m; during sowing, fossias are dug in field, sowing is carried out according to the planting spacing of 0.45-0.50m. The bottom fertilizer is applied in the space between seed curcumas, and covering soil is carried out. The technology can improve the yield of the medicinal materials of zedoary root and radix curcume longae produced by curcuma zedoaria by 15%-20%, increase the content of curcuminoids, the content of germacrone, the content of naphtha by 15%-20%, 16%-20% and 10%-15% respectively, and increase the economic benefit by 15%-20%.

The present invention relates to the new types of plant extract containing germacrone, curcumone and / or arylcurcumone, that is, nano liposome type, precursor liposome type long-cycle liposome type, long-cycle nano liposome type, nano microemulsion type, venous emulsion type, nano liposome sphere type and fat emulsion type.

The invention discloses a solanum lycopersicum fermentation broth extract as well as a preparation method and application thereof. The extract is prepared by fermenting a raw material solanum lycopersicum with lodderomyces elongisporus and then extracting aroma components from fermentation broth. Lodderomyces elongisporus is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.6645 and collection date of October 08, 2012. The solanum lycopersicum fermentation broth extract contains various aroma components, such as phenethyl alcohol, furfural, 5-hydroxymethylfurfural, germacrone, cis-3-hexenol, megastigmatrienone and the like. Flavoring smoke panel test results show that the extract can harmonize with cigarette aromas and can obviously improve and regulate cigarette aromas, refine alcohols and smoke, reduce the irritation, hide offensive odors, improve the smoking flavors of cigarettes and produce fresh special aroma.

The invention relates to a method for preparing germacrone, which belongs to the field of the preparation of traditional Chinese medicines. According to the method, the germacrone is extracted and prepared from raw materials which are rich in the germacrone. The preparation method comprises the following steps of: after primarily processing the raw materials which are rich in the germacrone, performing ultrasonic extraction by using an organic solvent at the temperature of below 45 DEG C; performing rotary evaporation and concentration to obtain extract; performing chromatography by using a silica gel-alumina composite column; performing gradient elution on the mixed solvent; recycling the solvent; and re-crystallizing by using petroleum ether-acetone or cyclohexane-ethanol to obtain the germacrone. By the preparation method, the germacrone has high yield; a closed system is adopted during extraction; solvent consumption is low; meanwhile, production safety is improved.

The invention provides an essence suitable for low-temperature non-combustion smoke products, a preparation method and an application thereof, and relates to the technical field of low-temperature non-combustion smoke products. The essence comprises components with antitussive and expectorant effect and the components with antitussive and expectorant effect is prepared from at least one of foliumArtemisiae Argyi oil, daurian rhododendron oil, oleum viticis negundo, alpha-asarone, Germacrone, P-Cymene, guaiacol sulfonic acid potassium, acetylcysteine, isofraxidine, esculetin, ligustilide, stachydrine, ligustrazine, imperatorin, isoimperatorin, Praeruptorin A, Praeruptorin B, Praeruptorin C, hygrine, and Cuscohygrine. The antitussive and expectorant active ingredient is natural active ingredient, which can volatilize and transfer to the total particulate matter of the smoke at atomization temperature, enrich the smoke and enhance the mouthfeel, and at the same time is partly absorbed bythe human body, so that the low-temperature non-combustible smoking product has the function of regulating the respiratory system function to a certain extent.

The invention discloses application of germacrone in preparation of a medicament for treating or preventing influenza viruses. The cell toxicity of the germacrone on madin-darby canine kidney (MDCK) cells is detected, and the result shows that CC50 is more than 250 microns. The influence of the germacrone on the expression of three antigens and the influence of germacrone on virus genome reproduction are detected, and the result shows that the germacrone formulation inhibits the expression of the virus antigens and the virus genome reproduction with dependency. The virus activity resistant mechanism of the germacrone is preliminarily researched. The virus activity resistance stage of the germacrone in a single reproduction period of the influenza viruses is researched by administration experiments of different time, and the result shows that the germacrone has inhibiting effect on adsorption, entry and early reproduction of the viruses. Moreover, the virus activity resistance of the germacrone to amantadine medicament resistant strains, B-type influenza virus strains and A-type influenza virus H3N2 subtype strains is detected, and the result shows that the germacrone has virus activity resistance to the three kinds of virus strains. The germacrone has the effects of relieving cough, relieving asthma and the like.

The invention discloses an extraction and enrichment method for a sesquiterpene component in curcuma wenyujin. The sesquiterpene component is a mixture of Germacrone, Furanodiene and beta-elemene. Themethod comprises the following steps of: 1) extraction: mixing curcuma wenyujin powder and ionic liquid water solution, carrying out microwave extraction to obtain suspension, carrying out centrifugation to obtain supernatant, and carrying out filtration to obtain filtrate, so as to obtain curcuma wenyujin extracting solution; and 2) enrichment: mixing lauryl sodium sulfate, a fluorinated carbonnanotube and water, carrying out ultrasonic treatment to obtain dispersion liquid, adding the dispersion liquid into the curcuma wenyujin extracting solution, carrying out vortex and filtration aftermixing, diluting the obtained solid, and collecting the dilution liquid to obtain enrichment solution. The method is simple and low in solvent dosage, is capable of effectively avoiding the using of toxic and harmful reagents, is green and environment-friendly, is capable of avoiding the loss of the low-boiling point sesquiterpene component due to high-temperature extraction, and is suitable for large-scale popularization and application.

The invention discloses a medicine composition for treating pneumonia. The medicine composition is prepared from garlic, coptidis rhizomes, magnetite, Chinese siphonostegia herb, radix sophorae flavescentis, germacrone and bergenin according to a proportion. The medicine composition can be prepared into various dosage forms according to conventional preparation technologies and is significant in treatment effect when used for treating pneumonia.

The invention discloses long-acting perfume and a preparation method thereof. The perfume is prepared from the following components in parts by weight: 5-8 parts of rose essential oil, 6-10 parts of flower-de-luce essential oil, 1-3 parts of germacrone, 30-80 parts of alcohol, 5-10 parts of glycerinum and 100-150 parts of water. The perfume has sweet floral fragrance and can be stored for a long time without fragrance fading, the fragrance can be kept for more than 3 days when the perfume is sprayed to skin, the effect is durable, and the problem of short fragrance holding time existing in theperfume field commonly can be solved. The preparation method is simple and feasible, and the perfume has stable fragrance and is suitable for large-scale industrial production.

The invention relates to a preparation method of germacrone oxobutyric acid. The preparation method is characterized by comprising the following step: reacting a germacrone reducing product I with substituted succinic anhydride to prepare the germacrone oxobutyric acid II. A compound II is synthesized by adopting the preparation method for the first time, the reaction condition is mild, the operation is simple, the raw material is easy to obtain, the cost is relatively low, the subsequent structure modification and transformation are facilitated since the germacrone oxobutyric acid contains along-chain carboxyl, and the preparation method has important theoretical and practical significance in developing a novel curcuma zedoaria antitumor drug.

The invention discloses a method for separating germacrone from Curcuma wenyujin volatile oil. The method comprises the following steps: fully mixing an aqueous solution of methyl-beta-cyclodextrin with n-hexane according to a volume ratio of 1:0.5-2, standing, carrying out liquid separation to respectively obtain an upper phase which is a mobile phase and a lower phase which is the stationary phase of a high-speed countercurrent chromatograph; and weighing the Curcuma wenyujin volatile oil, dissolving the Curcuma wenyujin volatile oil in a mixture containing the upper phase and the lower phase according to a volume ratio of 1:1, introducing the above obtained sample, carrying out high-speed countercurrent chromatographic separation, detecting through an ultraviolet detector, collecting corresponding effluents corresponding to peaks in the spectrogram of the ultraviolet detector, concentrating germacrone-containing effluents, and drying to prepare pure germacrone. The method has the advantages of simple operation, less solvent consumption, high efficiency, fastness, strong purposefulness and good separation effect.

The invention discloses a detection method for curcuma zedoaria in Fuping capsule. The detection method is characterized in that the curcuma zedoaria content is determined through gradient elution by using high performance liquid chromatography, wherein only the curcuma zedoaria in the Fuping capsule contains germacrone, such that the curcuma zedoaria content determination is exclusive. According to the present invention, the curcuma zedoaria content determination is performed in the Fuping capsule detection so as to effectively control the quality of the Fuping capsule.

The invention relates to a radix curcumae storage method, which comprises the following steps of (1) collecting side roots of fresh radix curcumae; eliminating fine roots, impurities and foreign materials; performing soaking by water for 5 to 10 min; brushing away soil and scar residues attached onto roots and stems; then performing flushing by flowing water for 0.5 to 1.5 min; (2) steaming the flushed radix curcumae for 1 to 2.5 h; (3) processing the radix curcumae by a far infrared drying method so that the water content is lower than 14 percent; (4) coating a glutin layer; then performing drying; (5) packaging the dried radix curcumae by a woven bag; putting the materials under the conditions of the temperature being 10 to 20 DEG C and the humidity being 51 to 53 percent. The method disclosed by the method has the advantages that curcuma zedoary oil and germacrone in the radix curcumae can be well retained; in the storage period, the moisture content increase speed and the reductionspeed of curcuma zedoary oil and germacrone content is slow; the effective storage time of the radix curcumae can be obviously increased.

The invention provides a technology for extracting Germacrone from Rhododendron thymifolium Maxim leaves with a freezing method and belongs to the technical field of extraction and separation of effective active ingredients of plants. The technology comprises steps as follows: the Rhododendron thymifolium Maxim leaves are smashed to form powder, and volatile oil is extracted with a vapor distillation method firstly; the volatile oil is dehydrated with anhydrous sodium sulfate and placed in a low-temperature environment for freezing, crystals are separated out and filtered, the crystals are dissolved with ethyl alcohol and recrystallized after being washed with ethyl alcohol, filtering and washing are performed again, and high-purity Germacrone is obtained. A high performance liquid chromatography analyzer determines that the purity of Germacrone extracted and separated with the technology is 95%-98%, the yield can be up to 0.20%-0.50%. The technology is simple, short in process, low in power consumption and cost and high in yield and product purity and requires simple equipment; water and ethyl alcohol are taken as solvents, so that the technology is environment-friendly and clean and has little pollution; industrial production can be realized.

The invention belongs to the technical field of medicines and particularly relates to application of (4S,5S)-germacrone-4,5-epoxide in preparation of antibacterial and anti-inflammatory drugs. Researches find that the (4S,5S)-germacrone-4,5-epoxide can be used for playing an anti-inflammatory effect by inhibiting expression levels of inflammation-related factors, has good antibacterial and anti-inflammatory effects, and is very suitable for being used as an active pharmaceutical ingredient of the antibacterial and anti-inflammatory drugs.

The invention relates to a quality evaluation method of a five-ingredient capsule for warming and activating collaterals and eliminating arthralgia. 15 ingredients including coumarin, baicalin, epimedin C, icariin, cinnamic acid, wogonoside, cinnamyl aldehyde, baicalein, cinnamyl alcohol, 2-methoxycinnamyl aldehyde, wogonin, baohuoside I, dehydrated icariin, germacrone and pachymic acid are selected as quality markers of the compound; full-wavelength scanning detection is performed, and UHPLC quality evaluation is performed on WWCC (Wuwei Wuntong arthralgia-eliminating capsule) under two wavelengths of 203nm and 270nm; after the analysis method is established and verified, the 15 quality markers in the WWCC sample are quantitatively analyzed, a WWCC fingerprint spectrum under 270nm is constructed, and the overall quality evaluation of the WWCC sample is completed. The method provides a unique idea for WWCC comprehensive quality evaluation, and provides reference and demonstration effects for traditional Chinese medicine compound overall quality evaluation.

The invention provides a composition. The composition is composed of furandiene and germacrone. The invention also discloses an application of the furandiene-germacrone composition in preparation of medicines for treating psoriasis. The composition has a remarkable inhibition effect on content of NO released by LPS-induced RAW264.7 cells. The composition has remarkable effect of inhibiting proliferation of HaCaT cells. In-vitro tests prove that furandiene and germacrone have a remarkable curative effect on psoriasis and can be used for preparing medicines for treating psoriasis.

The invention discloses application of germacrone in preparation of a medicament for treating or preventing influenza viruses. The cell toxicity of the germacrone on madin-darby canine kidney (MDCK) cells is detected, and the result shows that CC50 is more than 250 microns. The influence of the germacrone on the expression of three antigens and the influence of germacrone on virus genome reproduction are detected, and the result shows that the germacrone formulation inhibits the expression of the virus antigens and the virus genome reproduction with dependency. The virus activity resistant mechanism of the germacrone is preliminarily researched. The virus activity resistance stage of the germacrone in a single reproduction period of the influenza viruses is researched by administration experiments of different time, and the result shows that the germacrone has inhibiting effect on adsorption, entry and early reproduction of the viruses. Moreover, the virus activity resistance of the germacrone to amantadine medicament resistant strains, B-type influenza virus strains and A-type influenza virus H3N2 subtype strains is detected, and the result shows that the germacrone has virus activity resistance to the three kinds of virus strains. The germacrone has the effects of relieving cough, relieving asthma and the like.

The invention provides a method for extracting germacrone from rhododendron thymifolium leaf with a silica gel method, and relates to the technical field of plant effective active component extraction. According to the invention, rhododendron thymifolium leaf is crushed and is extracted with a steam distillation method, such that volatile oil is obtained; the volatile oil is dehydrated with anhydrous sodium sulfate, and is added into a silica gel column; elution is carried out with a mixed solvent of petroleum ether and ethyl acetate; part of the fraction is collected, crystallized and filtered; the crystals are washed with petroleum ether, and are dissolved with petroleum ether; recrystallization is carried out, and filtering and washing are carried out, such that high-purity germacrone is obtained. As a result of determination with high-performance liquid chromatography, the germacrone extracted and separated in the invention has a purity of 95-98% and a yield reaching 0.40-0.70%. The method provided by the invention has the advantage of simple process, low cost, high product yield and high product purity. The method is suitable for industrialized productions.

The invention provides a technology for extracting Germacrone from Rhododendron thymifolium Maxim leaves with a freezing method and belongs to the technical field of extraction and separation of effective active ingredients of plants. The technology comprises steps as follows: the Rhododendron thymifolium Maxim leaves are smashed to form powder, and volatile oil is extracted with a vapor distillation method firstly; the volatile oil is dehydrated with anhydrous sodium sulfate and placed in a low-temperature environment for freezing, crystals are separated out and filtered, the crystals are dissolved with ethyl alcohol and recrystallized after being washed with ethyl alcohol, filtering and washing are performed again, and high-purity Germacrone is obtained. A high performance liquid chromatography analyzer determines that the purity of Germacrone extracted and separated with the technology is 95%-98%, the yield can be up to 0.20%-0.50%. The technology is simple, short in process, low in power consumption and cost and high in yield and product purity and requires simple equipment; water and ethyl alcohol are taken as solvents, so that the technology is environment-friendly and clean and has little pollution; industrial production can be realized.

The invention relates to application of germacrone in preparation of medicines for preventing and treating psoriasis. The application is characterized in that the medicine consists of germacrone and amedically acceptable auxiliary material, wherein the content of the germacrone in the medicine is 0.1% to 50% in percentage by mass. The medicine has the function of resisting psoriasis, and can be used for preventing and treating the psoriasis.

The invention provides perfume with a fatigue relieving effect and a preparation method of the perfume. The perfume is prepared from 5 to 15 parts of base oil, 10 to 25 parts of composite extracting solution, 1 to 3 parts of xylitol, 0.5 to 3 parts of PEG-40 hydrogenated castor oil, 1 to 5 parts of trimethyl isopropyl butyramide, 3 to 8 parts of beta-ethyl phenylacrylate, 1 to 4 parts of germacrone, 5 to 20 parts of lemon essence, 1 to 3 parts of jasmine essential oil, 1 to 5 parts of michelia alba essential oil, 65 to 85 parts of ethanol and 5 to 10 parts of deionized water, wherein the base oil is selected from one or more of grape seed oil, evening primrose oil and sandalwood oil; and the composite extracting solution is an extracting solution of maca (lepidium meyenii walp.) and tribulus terrestris.

The invention discloses a germacrone alcoholization and biological resolution method. The method comprises the following steps: by taking germacrone extracted from cedar branches and leaves as a raw material, carrying out reduction with sodium borohydride to obtain a compound II, carrying out enzymatic kinetic resolution reaction on the compound II to obtain a compound III and a compound IV, or carrying out dynamic kinetic resolution to obtain a compound III of which the yield is more than 90%, and hydrolyzing the compound III to obtain a compound V. According to the invention, a latent chiral ketone group in germacrone is further changed to a chiral hydroxyl center so as to carry out further resolution. The method disclosed by the invention has the characteristics of simple operation, high product yield, good optical purity and the like.

The invention relates to a rapid center control testing method of Xingnaojing injection. The rapid center control testing method comprises the following steps of: (1) acquiring samples of primary distillate and secondary distillate in the preparation process of the Xingnaojing injection of different batches; (2) testing the areas of the curcuma zedoary diketone, the curcumenol and the Germacrone in the distillate samples and the total liquid phase peak areas by using a liquid chromatographic method, and testing the muscone peak areas in the distillate samples by using a gas chromatographic method; (3) acquiring near infrared spectrum data of the distillate samples; (4) constructing a model; and (5) testing the content of the curcuma zedoary diketone, the curcumenol, the Germacrone and the muscone in unknown distillate samples.