Process for extracting germacrone from rhododendron thymifolium leaf with silica gel method

Abstract

The invention provides a method for extracting germacrone from rhododendron thymifolium leaf with a silica gel method, and relates to the technical field of plant effective active component extraction. According to the invention, rhododendron thymifolium leaf is crushed and is extracted with a steam distillation method, such that volatile oil is obtained; the volatile oil is dehydrated with anhydrous sodium sulfate, and is added into a silica gel column; elution is carried out with a mixed solvent of petroleum ether and ethyl acetate; part of the fraction is collected, crystallized and filtered; the crystals are washed with petroleum ether, and are dissolved with petroleum ether; recrystallization is carried out, and filtering and washing are carried out, such that high-purity germacrone is obtained. As a result of determination with high-performance liquid chromatography, the germacrone extracted and separated in the invention has a purity of 95-98% and a yield reaching 0.40-0.70%. The method provided by the invention has the advantage of simple process, low cost, high product yield and high product purity. The method is suitable for industrialized productions.

Description

technical field

[0001] The invention relates to a method for extracting gemaconone from the leaves of Rhododendron thurium, in particular to a process for extracting gemaconone from the leaves of Rhododendron thurium through silica gel, and belongs to the technical field of extraction and separation of plant effective active ingredients. Background technique

[0002] Gemma ketone is the main active ingredient in the traditional Chinese medicine Curcuma and Curcuma. Pharmacological experiments have proved that gemmacone has obvious anticancer activity. It has obvious inhibitory effect on the proliferation of human lung cancer A549 cells, and can induce apoptosis of human lung cancer A549 cells; it can also inhibit the proliferation of liver cancer Bel7402 cells in vitro; it also has anti-herpes simplex, amantadine-resistant strains, type B Activity of influenza virus strains and H3N2 subtype strains of influenza A virus (CN102274206B); gemmacone has good preventive and thera...