The invention relates to a traditional Chinese medicine for treating bovine follicular cysts and a preparation method thereof, which can effectively solve the problem of medication for treating bovine follicular cysts. The technical solution is that the Chinese medicine is made of the following raw materials in weight percentage: Motherwort 1.4-1.8% , Caulis Spatholobus 1.3‑1.7%, Safflower 1.3‑1.7%, Ligustrum 0.8‑1.2%, Raspberry 0.8‑1.2%, Cuscuta 0.8‑1.2%, Lycium barbarum 0.8‑1.2%, Paeoniae Rubra 0.6‑1% , Radix Paeoniae Alba 0.6‑1%, Zeilan 0.6‑1%, Sappan 0.6‑1%, Liu Jinu 0.6‑1%, Achyranthes bidentata 0.6‑1%, Prunella 0.6‑1%, Bupleurum 0.3‑1% 0.5%, raw cattail 0.3-0.5%, active biochemical bacteria liquid 18-22% and deionized water 60-70%, the present invention has rich raw materials, easy preparation, low cost, convenient use, good therapeutic effect, and the cow is cured The post-mating success rate is high, and it is a great innovation in the treatment of bovine follicular cysts.