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Extraction and enrichment method for sesquiterpene component in curcuma wenyujin

A technology of warming turmeric and sesquiterpene, which is applied in the field of extraction and analysis of natural medicines, can solve the problems of low extraction rate of target components, non-compliance with green chemistry, serious environmental pollution, etc., achieve good chemical stability and thermal stability, and sample extraction method Simple, good adsorption performance

Active Publication Date: 2019-04-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these extraction techniques are simple to operate, a large amount of toxic and harmful organic reagents are used in the extraction process, which seriously pollutes the environment and does not conform to the concept of "green chemistry". The extraction time is long and the extraction rate of the target components is low.

Method used

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  • Extraction and enrichment method for sesquiterpene component in curcuma wenyujin
  • Extraction and enrichment method for sesquiterpene component in curcuma wenyujin
  • Extraction and enrichment method for sesquiterpene component in curcuma wenyujin

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Effect test

preparation example Construction

[0037] The specific steps of the preparation method of the Wen Curcuma reference substance mixed solution are: respectively accurately weigh an appropriate amount of gemaconone, furandadiene, and β-elemene reference substances, mix and dissolve them with methanol and set the volume to a 1mL volumetric flask to prepare the above-mentioned The final concentrations of various reference substances are respectively 1mg / mL, 1mg / mL, and 1mg / mL reference substance mixture.

[0038] When using dispersive micro-solid phase extraction for enrichment, the influence of the following parameters on the effect of dispersive micro-solid phase extraction was investigated:

[0039] (1) Selection of the type of adsorbent

[0040] In parallel, 4 parts of 50.47 mg sodium lauryl sulfate were added to 4 groups of 100 mL conical flasks with stoppers, and different kinds of adsorbents (silica gel, Al 2 o 3 , C 18 , 1 mg each of fluorinated carbon nanotubes) were added to four sets of Erlenmeyer flas...

Embodiment 1

[0065] (1) Extraction: add 0.5g of Curcuma curcuma source medicinal material powder into 10mL of 0.250M 1-decyl-3-vinylimidazole bromide ionic liquid, place the mixed solution in a microwave extractor, and at 60°C, Microwave extraction for 10 minutes, the extraction power is 200W, the suspension after microwave extraction is transferred to a 50mL centrifuge tube, centrifuged at 4000rpm for 20min to take the supernatant, the supernatant is filtered through a 0.22μm filter membrane, and the filtrate is the turmeric extract ;

[0066] (2) Add 50.47 mg of sodium lauryl sulfate and 1 mg of carbon fluoride nanotubes into a 100 mL Erlenmeyer flask with a stopper, add water to 10 mL, and mix well by ultrasonication for 15 minutes to obtain a dispersion of carbon fluoride nanotubes;

[0067](3) Enrichment: Take a 50mL centrifuge tube, add 1mL of Curcuma curcuma extract, then add 200μL of carbon fluoride nanotube dispersion, add water to 10mL; then place the centrifuge tube on a vortex ...

Embodiment 2

[0070] The specific reaction conditions are consistent with the method described in Example 1, except that the ionic liquid 1-decyl-3-vinylimidazole bromide is replaced by 1-hexyl-3-vinylimidazole bromide, 1-octyl-3-vinylimidazole bromide, 3-vinylimidazole, 1-dodecyl-3-vinylimidazole bromide.

[0071] image 3 A shows the histogram of the extraction effect under different types of ionic liquids. 1 and 2 in the figure represent different sesquiterpene components, respectively, gemmaconone and furadienes; in the figure [HVIM]Br is 1-hexyl bromide -3-vinylimidazole, [OVIM]Br is 1-octyl-3-vinylimidazole bromide, [C10VIM]Br is 1-decyl-3-vinylimidazole bromide, [C12VIM]Br is bromide 1-dodecyl-3-vinylimidazole.

[0072] The results show that as the alkyl chain length increases from C 6 increase to C 10 , the extraction efficiency is significantly improved, and when the alkyl chain length changes from C 10 increase to C 12 , the extraction efficiency is significantly reduced.

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Abstract

The invention discloses an extraction and enrichment method for a sesquiterpene component in curcuma wenyujin. The sesquiterpene component is a mixture of Germacrone, Furanodiene and beta-elemene. Themethod comprises the following steps of: 1) extraction: mixing curcuma wenyujin powder and ionic liquid water solution, carrying out microwave extraction to obtain suspension, carrying out centrifugation to obtain supernatant, and carrying out filtration to obtain filtrate, so as to obtain curcuma wenyujin extracting solution; and 2) enrichment: mixing lauryl sodium sulfate, a fluorinated carbonnanotube and water, carrying out ultrasonic treatment to obtain dispersion liquid, adding the dispersion liquid into the curcuma wenyujin extracting solution, carrying out vortex and filtration aftermixing, diluting the obtained solid, and collecting the dilution liquid to obtain enrichment solution. The method is simple and low in solvent dosage, is capable of effectively avoiding the using of toxic and harmful reagents, is green and environment-friendly, is capable of avoiding the loss of the low-boiling point sesquiterpene component due to high-temperature extraction, and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the technical field of extraction and analysis of natural medicines, and in particular relates to an extraction and enrichment method for sesquiterpene components in Curcuma curcuma. Background technique [0002] Curcuma wenyujin (Curcuma wenyujin Y.H.Chen et C.Ling) is a plant of the genus Curcuma longa in the Zingiberaceae family. It is one of the famous authentic medicinal materials "Zhe Bawei". Physiological effects such as anti-inflammation, anti-coagulation, anti-oxidation and liver protection. The main active ingredients of Wen Yujin include sesquiterpenoids and curcuminoids, among which sesquiterpenoids include gemmaconone, furadienes, β-elemenes, etc. Existing pharmacological and clinical studies have found that gemmacone, furadienes, and β-elemene all have antiviral and antitumor activities, and have inhibitory effects on liver cancer, gastric cancer, lung cancer, and breast cancer; Terpenes also have good therapeut...

Claims

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Application Information

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IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062
Inventor 楚楚王珊颜继忠
Owner ZHEJIANG UNIV OF TECH
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