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Tissue culture method for Curcuma wenyujin No.1

A technology of tissue culture and warming turmeric, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of seedling quality decline, long multiplication cycle, affecting multiplication efficiency, etc., to achieve rapid supply, accelerated promotion speed, and improved Effects on Planting Yield

Inactive Publication Date: 2011-01-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current cultivation method of Wenyujin No. 1 is stem propagation, which has the following defects: the annual reproduction rate is only about 25 times
[0004] Curcuma curcuma detoxification tissue culture technology research (Wang Hong, 2009, pharmaceutical biotechnology) discloses a kind of culture method of Curcuma curcuma, which has the following disadvantages: the reproductive coefficient of adventitious buds is low, only 2 to 4 times; length, about 4 weeks; and during the proliferation of adventitious buds, root primordia are induced and a large number of root systems develop. The simultaneous growth of adventitious buds and adventitious roots not only seriously affects the proliferation efficiency, but also makes the subculture division of adventitious buds difficult to operate. Finally, the quality of seedlings will decline, and the survival rate of transplanting will be low, only reaching 85%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: A kind of tissue culture method of Wen Yujin No. 1, carries out following steps successively:

[0029] 1) Put the robust and plump Wenyujin No. 1 tubers without disease spots and galls into a biochemical incubator for germination until they grow into small buds with a height of 0.5-2.0 cm; the culture conditions are: 26-28°C dark culture.

[0030] 2) Cut the above-mentioned small buds and transfer them into gauze bags, rinse them with sterile water for 1 to 2 hours.

[0031] 3), after the small buds after the above-mentioned flowing water washing are routinely disinfected (that is, 0.1% w / v HgCl 2 After 6 to 10 minutes of treatment, rinse with sterile water 5 to 8 times, then blot dry with sterile filter paper), peel off 3-5 mm-sized stem tips as explants and inoculate them on sterile seedling medium for cultivation; The culture conditions are: 16 hours of light, light intensity 30-40 μmol m -2 .s -1 , the temperature is 27±1°C; 8 hours of dark cultivat...

Embodiment 2

[0041] Embodiment 2, a kind of tissue culture method of Wen Yujin No. 1, only the formula of culture medium is changed as follows, all the other are the same as embodiment 1:

[0042] The culture medium for sterile seedlings is: 1 / 2 MS basic medium + white sugar 20g / l + agar 9g / l, and the pH is 5.5-6.0.

[0043] Adventitious bud induction medium is: MS basic medium + TDZ 0.05mg / l + white sugar 30g / l + agar 4g / l, pH is 5.5-6.0.

[0044] Proliferation medium is: MS basic medium + TDZ 0.45mg / l + white sugar 30g / l + agar 4g / l, pH 5.5-6.0.

[0045] The rooting medium is: 1 / 2MS basic medium + white sugar 15g / l + agar 10g / l, the pH is 5.5-6.0.

[0046] According to the above method, the test-tube plantlets can be obtained in only 45-50 days; therefore, a large amount of test-tube plantlets can be obtained for a long time by adopting the method of the present invention.

Embodiment 3

[0047] Embodiment 3, a kind of tissue culture method of Wen Yujin No. 1, only the formula of culture medium is changed as follows, all the other are the same as embodiment 1:

[0048] The culture medium for sterile seedlings is: 1 / 2 MS basic medium + white sugar 30g / l + agar 4g / l, and the pH is 5.5-6.0.

[0049] Adventitious bud induction medium is: MS basic medium + TDZ 0.5mg / l + white sugar 20g / l + agar 9g / l, pH is 5.5-6.0.

[0050] Proliferation medium is: MS basic medium + TDZ 0.06mg / l + white sugar 20g / l + agar 9g / l, pH is 5.5-6.0.

[0051] The rooting medium is: 1 / 2MS basic medium + white sugar 20g / l + agar 4g / l, the pH is 5.5-6.0.

[0052] According to the above method, the test-tube plantlets can be obtained in only 45-50 days; therefore, a large amount of test-tube plantlets can be obtained for a long time by adopting the method of the present invention.

[0053] In summary, the tissue culture method of Wenyujin No. 1 of the present invention has the following chara...

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Abstract

The invention discloses a tissue culture method for curcuma wenyujin No.1. The method comprises the following steps in turn: 1) putting a tuberous root of the robust curcuma wenyujin No.1 without scab or insect gall into a biochemical lncubator, and carrying out pregermination; 2) cutting budlet, and washing the cut budlet by running water; 3) after carrying out conventional disinfection on the budlet, peeling stem tip of 3 to 4mm for culturing; 4) inoculating the cut stem tip serving as a plantlet I to an adventitious bud inducing culture medium for culturing; 5) cutting an adventitious bud I induced on the plantlet I to serve as a plantlet II for propagation culture; 6) cutting an adventitious bud II growing on the plantlet II to serve as a plantlet III for rooting culture; and 7) obtaining a seedling for bottle planting when the plantlet III grows to the height of between 4 and 8cm and has at least 3 roots longer than 2cm. A mass of curcuma wenyujin No.1 tissue cultured seedlings can be obtained quickly by adopting the method.

Description

technical field [0001] The invention relates to a tissue culture method of Wenyujin No. 1. Background technique [0002] Wen Yujin has various pharmacological activities such as anti-tumor, anti-inflammation, anti-oxidation, and anti-AIDS. It is clinically used to treat lung cancer, liver cancer, breast cancer, and hydrothorax. It has a history of more than 1,000 years of cultivation in southern Zhejiang. It is mainly produced in Ruian, Zhejiang, and it is mostly cultivated and less wild. However, due to years of planting and chaotic management, as well as the increasingly serious environmental pollution and soil nutrient barrenness, the germplasm and quality of Wenyujin medicinal materials have deteriorated, which has seriously affected the development of Wenyujin medicinal materials industry. [0003] As one of the varieties of Wenyujin, Wenyujin No. 1 can be provided by Yueqing Yuansheng Traditional Chinese Medicine Planting Co., Ltd. The current cultivation method of W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈丽闽毛碧增
Owner ZHEJIANG UNIV
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