Method for removing Leifsonia xyli subsp.xyli and quickly breeding health cane seedling

Abstract

The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. The method has the advantages that: dwarfing disease pathogenic bacteria of the perennial root of the cane seedling can be thoroughly removed, annually propagation coefficient can reach as high as more than 10 thousand times, furthermore, the seedling has no variation, has the advantages of being fast, high-efficient and safe, and is suitable for the industrialized and standardized production of the cane healthy seedlings on scale.

Description

technical field [0001] The present invention relates to the reproduction and production of sugarcane seedlings, in particular to the technology for removing dwarfing pathogens of sugarcane perennial roots Background technique [0002] Sugarcane (Saccharum.spp) is the main sugar crop and a biomass crop with good development prospects in my country. Sugarcane ratoon dwarf disease (RSD) is a worldwide important sugarcane disease, and it is also a major sugarcane disease in my country's sugarcane regions. 15%, the loss of ratoon cane yield is 20% to 25%, and affects the life of rattan cane, which is one of the important reasons for the degradation of sugarcane varieties. The pathogenic bacteria of sugarcane RSD live in the vascular bundles of sugarcane stems, and there is no effective chemical control method; at the same time, due to the lack of resistant source materials, sugarcane RSD disease-resistant breeding has not achieved much, and no production varieties with better res...

AI Technical Summary

Problems solved by technology

The early traditional method of removing RSD bacteria from sugarcane is soaking seeds in hot water at 50°C for 2-3 hours or treating sugarcane seeds with hot air at 54-58°C for 8 hours, which can kill RSD pathogens in susceptible sugarcane stems to a certain extent and greatly reduce RSD. However, the above-mentioned heat treatment method has the disadvantages of not being able to completely remove RSD germs, easily damaging sugarcane buds, difficult to control water temperature, unstable degerming effect, and low seedling reproduction coefficient. Large-scale promotion and application in production

In recent years, there have also been reports on the use of heat treatment combined with shoot apical meristem (less than 1 mm) culture to remove sugarcane RSD pathogens, but the explants cut by this method are very small and need to be cut under a dissecting microscope, which is difficult to operate, and due to the The body is very small, resulting in a high rate of phenol pollution during the cultivation process, an extremely low rate of induced seedling formation, and high production costs, making it difficult to popularize and apply in production