Induction method of hairy roots of Curcuma wenyujin

A hairy root and warm turmeric technology is applied in the field of hairy root induction of warm turmeric, which can solve the problems of difficult cultivation and low yield of root medicinal plants.

Active Publication Date: 2020-09-04
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

The purpose of the present invention is to provide a method for efficiently inducing the hairy roots of Curcuma curcuma to overcome the current probl

Method used

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  • Induction method of hairy roots of Curcuma wenyujin
  • Induction method of hairy roots of Curcuma wenyujin
  • Induction method of hairy roots of Curcuma wenyujin

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Embodiment

[0027] 1. Preparation of infection solution for inducing hairy roots

[0028] 1) Preparation of Agrobacterium infection solution: Streak culture of wild-type Agrobacterium rhizogenes K599 stored at -80°C on LB medium containing 50 mg / L Str at 28°C; Cultivate overnight in LB liquid medium containing 50 mg / L Str. When the Agrobacterium grows to a bacterial liquid OD value of about 1.0, take 1 mL bacterial liquid in a 1.5 mL centrifuge tube, centrifuge, and remove the supernatant; use MS liquid Suspend the bacteria in the culture medium, centrifuge and remove the supernatant; then dilute with MS liquid culture medium to an OD value of about 0.1, and use it as an Agrobacterium infection solution.

[0029] 2) Preparation of endophyte infection solution

[0030] The warm endophyte WYJ-E13 stored at -80°C was streak cultured on the PDA medium containing 50 mg / L Str, at 28°C; the cultured single colony was placed on the PDA liquid medium containing 50 mg / L Str Cultivate overnight in...

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Abstract

The invention discloses an induction method of hairy roots of Curcuma wenyujin. The induction method comprises the following steps: (1) preparation of infecting liquid, (2) culture of living tissue-cultured seedlings, (3) induction of the hairy roots, and (4) bacteria-free culture and reproduction of the hairy roots. According to the induction method, the tissue-cultured seedlings are infected byvirtue of endophytes of the Curcuma wenyujin and are taken as receptors of Agrobacterium rhizogenes, and stems of the living tissue-cultured seedlings of the Curcuma wenyujin are infected by virtue ofthe living Agrobacterium rhizogenes so as to effectively induce the hairy roots, so that a technical support is provided for growing medicinal components by virtue of a hairy root induction system ofthe Curcuma wenyujin.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation, and in particular relates to a method for inducing hairy roots of warm turmeric. Background technique [0002] Wen Yujin ( Curcuma wenyujin Y.H. Chen et C. Ling) is a plant of the genus Curcuma longa in the Zingiberaceae family, mainly distributed in Wenzhou, Zhejiang Province, and the whole plant can be used for medicinal purposes. The medicinal material "Wenyujin" processed from its roots is one of the famous authentic medicinal materials "Zhejiang Eight Flavors" in Zhejiang Province. According to the different medicinal parts and processing methods, the underground rhizomes of the turmeric plant can be processed into three different medicinal materials: the roots are boiled and dried and called "curcuma", the lateral rhizomes are cut into thick slices and dried, and they are called "turmeric slices". The rhizome is boiled and dried and called "warm curcuma". ...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H4/00A01H5/06A01H6/00
CPCC12N15/82A01H4/00C12N15/8205
Inventor 徐正贤向太和江洁罗灶霞李江山
Owner HANGZHOU NORMAL UNIVERSITY
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