DNA origami-based construction method and application of precise recognition targeted nano-carrier
A nanocarrier and construction method technology, applied in the field of medicine, can solve the problems of inability to achieve accurate identification and weak anti-tumor effect, and achieve the effect of enhancing anti-tumor effect, enhancing biotherapeutic effect, and delaying release.
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[0036] A construction method for precise identification of targeted nanocarriers based on DNA origami. The steps are as follows:
[0037] (1) Preparation of scaffold DNA (M13mp18 single-stranded DNA (N4040S), staple single-stranded DNA and special staple single-stranded DNA as materials for constructing the carrier (staple single-strand purchased from Shenggong Bioengineering (Shanghai) Co., Ltd. Co., Ltd., scaffolding DNA was purchased from NEB, USA); Designed and synthesized specific nucleic acid aptamer DNA (C2NP), and made a linker between the special staple single-stranded DNA and the nucleic acid aptamer DNA. The structure formed by the principle of complementary base pairing between the specific oligonucleotide sequence on the single-stranded DNA of the staple and the aptamer DNA;
[0038] (2) Dissolve the staple single-stranded DNA, special staple single-stranded DNA and scaffold DNA obtained in step (1) in 1×TAE / Mg 2+ Obtain the reaction solution from the solution and perf...
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