Embryoid induction method taking polyphylla bud axis as explant

Abstract

The invention discloses an embryoid induction method taking a polyphylla bud axis as an explant. The method comprises the following steps: firstly, carrying out disinfection and pre-cultivation on an un-germinated bud on polyphylla rhizome, and then cutting off coleoptile of the bud of which the volume obviously expands after pre-cultivation; inserting the bud axis into a callus-inducing medium to cultivate from the center after cutting in length, and then cultivating the callus tissue generated by the bud axis to obtain polyphylla embryonic tissues; finally carrying out induction cultivation on the embryonic tissues, so as to generate white spherical embryoids. By adopting the embryoid induction method, a new path is supplied for cultivation of excellent variety of the polyphylla and massive and rapid propagation of tissue culture seedlings; mass production of polyphylla plants at low cost within a short period of time can be achieved.

Description

technical field

[0001] The invention relates to a method for tissue culture of a polyphylla plant, in particular to a method for inducing an embryoid body using the bud axis of a polyphylla plant as an explant. Background technique

[0002] Chinese Pharmacopoeia stipulates that Paris polyphylla var. yunnanensis is the rhizome of Paris polyphylla var. yunnanensis and Paris polyphylla var. chinensis. The former is mainly produced in Yunnan, and the latter is mainly produced in Sichuan. The earliest record in "Shen Nong's Materia Medica" is Huachonglou. Zhonglou has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain, promoting blood circulation and removing stasis, cooling liver and relieving convulsions.

[0003] In recent years, due to the discovery that Chonglou has anti-virus and anti-tumor effects, the demand has increased greatly. Traditional Chinese medicinal materials of Chonglou have been provided with wild resources for medicinal ...