Method for rapidly identifying wheat fusarium stem rot

Abstract

The invention relates to a method for rapidly identifying wheat fusarium stem rot. The method comprises the following steps: 1, preparation of fusarium graminearum spore suspension; 2, germinating wheat seeds at a room temperature till the coleoptile length is 1.4-1.6 cm; 3, cutting 2-4 mm from the top end of coleoptile, taking the fusarium graminearum spore suspension of 2-4 microliters and inoculating at a coleoptile wound; 4, culturing in an illumination incubator for 7 days and measuring the coleoptile lesion length. The method provided by the invention has the beneficial effects that culture conditions are simple; the wheat fusarium stem rot inoculation is simple and rapid, the absorption is more rapid due to the existence of the coleoptile wound, the resistance level of the wheat fusarium stem rot can be identified within a shorter time, expensive equipment is not required; a large number of repetitions can be performed at a time, and the statistical method is high in accuracy, low in experiment cost, labor and force-saving, good in identification effect on wheat fusarium stem rot resistance and suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of plant protection, in particular to a method for rapidly identifying wheat Fusarium stem rot. Background technique [0002] Wheat head blight is a worldwide fungal disease caused by Fusarium fungus. In recent years, due to global warming and changes in farming systems and farming methods, wheat head blight has been aggravating and becoming widespread year by year, posing a serious threat to agricultural production. In addition to causing ear rot (ear rot, wheat scab) at the ear stage, wheat head blight can also occur at the seedling stage (stem rot, crown rot), causing symptoms such as seedling withering and base rot. At present, extensive and in-depth research on ear rot of wheat head blight has been carried out, and remarkable achievements have been made, but the research on wheat seedling disease caused by Fusarium has not yet attracted attention in my country. The research on stem rot There are few st...

AI Technical Summary

Problems solved by technology

[0003] Abroad, Tian o Tianman and Wil Tianermuth established a kind of wheat scab seedling resistance (stem rot) identification method in 1987, and Wil Tianermuth and Mcnamara improved this method in 1994, However, both of these methods require field inoculation. Due to the interference of factors such as the amount of inoculation, residual pathogens in the field, field humidity and temperature, etc., the repeatability of field identification results is often poor.

Wallwork et al. (2004), Mitter et al. (2006) and Li et al. (2008) all proposed methods for identifying wheat seedling stalk rot in greenhouses. These methods are effective, but there are complicated procedures, time-consuming and labor-intensive procedures, and experiments large error

Chinese scientists have also studied the identification method of wheat stem rot caused by Fusarium on the basis of previous research, and found that the "cotton ball inoculation method" has a significant difference in disease index among resistant materials, which can be used as an anti-corrosion method for wheat stem rot. However, this method has the characteristics of difficulty in inoculation and long identification period

Zhou Miaoping (2016) established a resistance evaluation method based on fluorescent quantitative PCR to measure the daily NA content of Fusarium graminearum in the basal stalks by fluorescent quantitative PCR and to analyze the correlation with the average disease level of stem rot. The method has the characteristics of high requirements for instruments and equipment and high post-appraisal costs