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A kind of preservation method of fusarium spore

A preservation method, Fusarium graminearum technology, applied in the field of plant protection, can solve problems such as time-consuming, and achieve high application value, good effect, and convenient and fast use process

Active Publication Date: 2021-08-24
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The problem solved by the present invention is that the currently preserved Fusarium needs to be reactivated when used, which takes a long time

Method used

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  • A kind of preservation method of fusarium spore
  • A kind of preservation method of fusarium spore

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Embodiment 1

[0038] The present embodiment provides a method for preserving Fusarium spores, the method comprising:

[0039] S1-1: Take the Fusarium graminearum strain Huanggang No. 1 stored at -20°C, inoculate it on a potato dextrose agar (PDA) plate, and culture it in the dark for 3 days in an incubator at 28°C to carry out the isolation of Fusarium graminearum. activation.

[0040]S1-2: Take the activated mycelium of Fusarium spp., inoculate it in the liquid medium of mung bean soup with a concentration of 6%, and cultivate it under light at 28°C for 5 days with shaking to produce conidia, and then culture the spores of Fusarium spp. to obtain Fusarium spp. Bacteria solution.

[0041] In order to ensure that the concentration of Fusarium spores in the prepared Fusarium bacterium liquid meets the inoculation requirements, the concentration of Fusarium spores is detected by microscope inspection from time to time during the shaking culture, and when the concentration of Fusarium spores i...

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Abstract

The invention provides a method for preserving Fusarium spores, which relates to the technical field of plant protection, comprising the following steps: using Fusarium strains as raw materials to cultivate Fusarium spores to obtain a Fusarium liquid; filtering the Fusarium liquid, and After the filtrate is centrifuged, remove the supernatant to obtain the Fusarium spores; adjust the concentration of the Fusarium spores to the working concentration to obtain the working bacterial liquid; take the working volume of the working bacterial liquid into the second centrifuge tube, centrifuge, and remove the supernatant solution to obtain spore precipitates; air-dry the second centrifuge tube to obtain dry spore precipitates; seal the second centrifuge tube and store at -20°C. The method for preserving Fusarium spores provided by the present invention, on the one hand, the preserved Fusarium spores can be taken as needed, on the other hand, the volume of double distilled water added does not need to be recalculated during use, and the prepared working bacteria The concentration of Fusarium spores in the liquid is controllable, quick and convenient, saving time and effort.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for preserving Fusarium spores. Background technique [0002] Fusarium (Fusarium) widely exists in various environments, ubiquitously present in soil and animal and plant organisms, and has a very wide geographical distribution; Fusarium is easy to infect a variety of crops, especially food crops, for example, the invasion of Fusarium Dyeing can cause wheat scab, wheat root rot and corn ear rot. At the same time, Fusarium will produce a variety of toxins during the infection process, including trichothecene compounds, fumonisin, zearalenone, etc. These toxins enter the human food chain through feed, food, etc. Safety brings great hidden dangers (Bai G H, Plattner R, Desjardins A, et al. Resistance to Fusarium head blight and deoxynivalenol accumulation inwheat [J]. Plant Breeding, 2001, 120 (1): 1-6.). [0003] At present, the incidence area of ​​wheat head bl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/77
CPCC12N1/04
Inventor 刘易科高春保朱展望邹娟舒军朱光佟汉文陈泠张宇庆
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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