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Preservation method of fusarium spores

A method of preservation, Fusarium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, preservation of microorganisms, etc., can solve the problems of time-consuming, etc., and achieve the effect of simple preparation method, improved accuracy, and easy operation

Active Publication Date: 2019-12-24
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The problem solved by the present invention is that the currently preserved Fusarium needs to be reactivated when used, which takes a long time

Method used

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  • Preservation method of fusarium spores
  • Preservation method of fusarium spores

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Embodiment 1

[0038] The present embodiment provides a method for preserving Fusarium spores, the method comprising:

[0039] S1-1: Take the Fusarium graminearum strain Huanggang No. 1 stored at -20°C, inoculate it on a potato dextrose agar (PDA) plate, and culture it in the dark for 3 days in an incubator at 28°C to carry out the isolation of Fusarium graminearum. activation.

[0040]S1-2: Take the activated mycelium of Fusarium spp., inoculate it in the liquid medium of mung bean soup with a concentration of 6%, and cultivate it under light at 28°C for 5 days with shaking to produce conidia, and then culture the spores of Fusarium spp. to obtain Fusarium spp. Bacteria solution.

[0041] In order to ensure that the concentration of Fusarium spores in the prepared Fusarium bacterium liquid meets the inoculation requirements, the concentration of Fusarium spores is detected by microscope inspection from time to time during the shaking culture, and when the concentration of Fusarium spores i...

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Abstract

The invention provides a preservation method of fusarium spores, and relates to the technical field of plant protection. The preservation method comprises the following steps of performing fusarium spores culture by using fusarium strains as raw materials so as to obtain fusarium bacterial liquid; filtrating the fusarium bacterial liquid, centrifuging filtrate, and removing supernatant so as to obtain the fusarium spores; adjusting the concentration of the fusarium spores to working concentration so as to obtain working bacterial liquid; taking the working bacterial liquid of working volume, loading the taken working bacterial liquid into a second centrifuging tube, performing centrifuging, and removing supernatant so as to obtain spore precipitate; performing air drying on the second centrifuging tube so as to obtain dried spore precipitate; and sealing the second centrifuging tube, and performing preservation under the condition of minus 20 DEG C. According to the preservation methodof fusarium spores provided by the invention, on one hand, the preserved fusarium spores can be taken at any time at needed, on the other hand, the volume of added redistilled water does not need tobe recalculated in the use process of the preserved fusarium spore, the concentration of the fusarium spores in the prepared working bacterial liquid is controllable, and the preservation method is quick, convenient, time-saving and labor-saving.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for preserving Fusarium spores. Background technique [0002] Fusarium (Fusarium) widely exists in various environments, ubiquitously present in soil and animal and plant organisms, and has a very wide geographical distribution; Fusarium is easy to infect a variety of crops, especially food crops, for example, the invasion of Fusarium Dyeing can cause wheat scab, wheat root rot and corn ear rot. At the same time, Fusarium will produce a variety of toxins during the infection process, including trichothecene compounds, fumonisin, zearalenone, etc. These toxins enter the human food chain through feed, food, etc. Safety brings great hidden dangers (Bai G H, Plattner R, Desjardins A, et al. Resistance to Fusarium head blight and deoxynivalenol accumulation inwheat [J]. Plant Breeding, 2001, 120 (1): 1-6.). [0003] At present, the incidence area of ​​wheat head bl...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/77
CPCC12N1/04
Inventor 刘易科高春保朱展望邹娟舒军朱光佟汉文陈泠张宇庆
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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